Rapid Visual Detection of Lead and Mercury via Enhanced Crosslinking Aggregation of Aptamer-Labeled Gold Nanoparticles

Berlina, Anna N.; Zherdev, Anatoly V.; Придворова, С. М.; Gaur, M. S.; Dzantiev, Boris B.

doi:10.1166/jnn.2019.16575

Cited by 27 publications

(15 citation statements)

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“…Whereas, many of the sensors described for the detection of heavy metal focus on one particular analyte, many researchers also report multiplex detection of multiple heavy metal ions. The described sensors ranged in complexity from simple fluorescent systems which rely on the formation of a metal dependent non-canonical base pair (either T-Hg 2+ -T or C-Ag + -C) to nanoparticle and electrochemical based methods (Zhang et al, 2018c;Abu-Ali et al, 2019;Berlina et al, 2019). A sensor which made use of metal stabilized base-pairing boasted detection limits of 5 × 10 −8 and 9.3 × 10 −10 mol/L for Hg 2+ and Ag + , respectively (Zhang et al, 2018c).…”

Section: Multiplex Detection Of Metal Ionsmentioning

confidence: 99%

Aptamer-Based Biosensors for Environmental Monitoring

2020

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Due to their relative synthetic and chemical simplicity compared to antibodies, aptamers afford enhanced stability and functionality for the detection of environmental contaminants and for use in environmental monitoring. Furthermore, nucleic acid aptamers can be selected for toxic targets which may prove difficult for antibody development. Of particular relevance, aptamers have been selected and used to develop biosensors for environmental contaminants such as heavy metals, small-molecule agricultural toxins, and water-borne bacterial pathogens. This review will focus on recent aptamer-based developments for the detection of diverse environmental contaminants. Within this domain, aptamers have been combined with other technologies to develop biosensors with various signal outputs. The goal of much of this work is to develop cost-effective, user-friendly detection methods that can complement or replace traditional environmental monitoring strategies. This review will highlight recent examples in this area. Additionally, with innovative developments such as wearable devices, sentinel materials, and lab-on-a-chip designs, there exists significant potential for the development of multifunctional aptamer-based biosensors for environmental monitoring. Examples of these technologies will also be highlighted. Finally, a critical perspective on the field, and thoughts on future research directions will be offered.

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Section: Multiplex Detection Of Metal Ionsmentioning

confidence: 99%

Aptamer-Based Biosensors for Environmental Monitoring

2020

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“…Conjugation of GNPs was provided with the use of 3 µM of PAO. This concentration was chosen in accordance with our previous study [21] to stabilize GNPs. The prepared native and PAO-modified GNPs were characterized by absorbance spectroscopy and TEM ( Figure 2).…”

Section: Characterization Of Pao-gnps Conjugatementioning

confidence: 99%

“…In addition, the optical density at 620 nm was measured. This wavelength has been chosen in previous studies on colorimetry [21]. According to experimental data, when comparing the optical densities at 595 and 620 nm for the entire studied range of metal ions, it was revealed that the maximum signal (A = 0.8) for antimony ions was reached at 620 nm.…”

Section: Colorimetric Detection Of Antimony (Iii)mentioning

confidence: 99%

“…Alternatively, the colorimetric approaches based on modified gold nanoparticles (GNPs) are widely applied due to their unique optical properties [20]. Aggregation of GNPs modified with receptor molecules after the addition of metal ions can change the original color of GNPs from red to blue [21][22][23]. However, selective induction of the aggregation should be based on specific recognition of the target metal ion.…”

Section: Introductionmentioning

confidence: 99%

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Colorimetric Technique for Antimony Detection Based on the Use of Gold Nanoparticles Conjugated with Poly-A Oligonucleotide

et al. 2019

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A simple and rapid positive–negative colorimetric approach to determine the presence of antimony ions based on the use of gold nanoparticles conjugated with oligonucleotide (poly-A sequence) is developed. Colorimetric measurements reveal that the aggregates of modified gold nanoparticles were afforded after adding antimony ions, thus changing the solution color from pink to blue. The results of aptamer’s interaction on the gold nanoparticle surface with the target analyte can be detected either by photometry or by the naked eye. The realized assay provides rapid (2 min), sensitive (detection limit 10 ng/mL), specific, and precise (variation coefficient less than 3.8%) detection of antimony (III) in drinking water.

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“…Conjugation of GNPs was provided with the use of 3 µ M of PAO. This concentration was chosen in accordance with our previous study [21] to stabilize GNPs. The prepared native and PAOmodified GNPs were characterized by absorbance spectroscopy, and TEM ( Figure 2).…”

Section: Characterization Of Pao-gnps Conjugatementioning

confidence: 99%

Colorimetric Technique for Antimony Detection Based on the Use of Gold Nanoparticles Conjugated with Poly-A Oligonucleotide

Berlina¹,

Komova²,

Zherdev³

et al. 2019

Preprint

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A simple and rapid positive–negative colorimetric approach to determine the presence of antimony ions based on the use of gold nanoparticles conjugated with oligonucleotide (poly-A sequence) is developed. Colorimetric measurements reveal that the aggregates of modified gold nanoparticles were afforded after adding antimony ions, thus changing the solution color from pink to blue. The results of aptamer’s interaction on the gold nanoparticle surface with target analyte can be detected either by photometry or by the naked eye. The realized assay provides rapid (2 min), sensitive (detection limit 10 ng/mL), specific, and precise (variation coefficient less than 3.8%) detection of antimony (III) in drinking water

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Rapid Visual Detection of Lead and Mercury via Enhanced Crosslinking Aggregation of Aptamer-Labeled Gold Nanoparticles

Cited by 27 publications

References 0 publications

Aptamer-Based Biosensors for Environmental Monitoring

Aptamer-Based Biosensors for Environmental Monitoring

Colorimetric Technique for Antimony Detection Based on the Use of Gold Nanoparticles Conjugated with Poly-A Oligonucleotide

Colorimetric Technique for Antimony Detection Based on the Use of Gold Nanoparticles Conjugated with Poly-A Oligonucleotide

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