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Cited by 31 publications
(8 citation statements)
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References 14 publications
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“…The sample was used immediately. (3) Previously frozen Rhesus monkey urine donated by Wyeth Laboratories, collected on the fourth day of the administration of benoxaprofen, 37.5 mg/(kg-day). The pH of this sample was 3.3, having been collected in 2.2 M citric acid.…”
Section: Methodsmentioning
confidence: 99%
“…The sample was used immediately. (3) Previously frozen Rhesus monkey urine donated by Wyeth Laboratories, collected on the fourth day of the administration of benoxaprofen, 37.5 mg/(kg-day). The pH of this sample was 3.3, having been collected in 2.2 M citric acid.…”
Section: Methodsmentioning
confidence: 99%
“…The original bilirubin used to prepare the DME contained similar amounts of the IIIa and XIIIa isomers (16). The identity, structure, and purity of the crystals were verified by mass spectrometry using a CEC 21-110 double-focusing mass spectrometer with a direct probe inlet system (17). No evidence of contamination of either mono-or dimethoxy-DME or bilirubin was detected.…”
Section: Methodsmentioning
confidence: 98%
“…From other plates which were simultaneously chromatographed, the corresponding yellow tetrapyrrole pigments were scraped and eluted into methanol and water. The bilirubin equivalent was determined colorimetrically by their conversion to sulfanilyl azo-dipyrrole pigments and the glucuronic acid content was determined by the napthorescorcinol method (17).…”
Section: In Vivo Perfusionsmentioning
confidence: 99%
“…Addition of NaCl further increased the extraction yield. Methanol was replaced by ethanol to minimize the risk of transmethylation of bilirubin conjugates in the acidic medium (17). The added ascorbic acid effectively protected the bilirubins from oxidative conversion to verdins as shown by the absence of biliverdin on thin-layer chromatographic analysis.…”
Section: Extraction and Thin-layer Chromatographymentioning
confidence: 99%