Rapid stimulation of a soybean protein-serine kinase that phosphorylates a novel bZIP DNA-binding protein, G/HBF-1, during the induction of early transcription-dependent defenses

Dröge‐Laser, Wolfgang; Kaiser, Annette; Lindsay, William P.; Halkier, Barbara Ann; Loake, Gary J.; Doerner, Peter; Dixon, Richard A.; Lamb, Chris

doi:10.1093/emboj/16.4.726

Cited by 169 publications

(119 citation statements)

References 75 publications

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“…In addition, adjacent G-and H-boxes in the promoter of the common bean chs15 gene are bound by transcription factor G/HBF-1. This protein is phosphorylated upon elicitor treatment (Drö ge-Laser et al, 1997). The presence of these motifs in the PvACCase promoter region suggests coordinated regulation with other genes for phenylpropanoid synthesis.…”

Section: Discussionmentioning

confidence: 97%

Hormonal and Stress Induction of the Gene Encoding Common Bean Acetyl-Coenzyme A Carboxylase

2006

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Regulation of the cytosolic acetyl-coenzyme A carboxylase (ACCase) gene promoter from common bean (Phaseolus vulgaris) was studied in transgenic Arabidopsis (Arabidopsis thaliana) plants using a β-glucuronidase (GUS) reporter gene fusion (PvACCase∷GUS). Under normal growth conditions, GUS was expressed in hydathodes, stipules, trichome bases, flowers, pollen, and embryos. In roots, expression was observed in the tip, elongation zone, hypocotyl-root transition zone, and lateral root primordia. The PvACCase promoter was induced by wounding, Pseudomonas syringae infection, hydrogen peroxide, jasmonic acid (JA), ethylene, or auxin treatment. Analysis of PvACCase∷GUS expression in JA and ethylene mutants (coronatine insensitive1-1 [coi1-1], ethylene resistant1-1 [etr1-1], coi1-1/etr1-1) suggests that neither JA nor ethylene perception participates in the activation of this gene in response to wounding, although each of these independent signaling pathways is sufficient for pathogen or hydrogen peroxide-induced PvACCase gene expression. We propose a model involving different pathways of PvACCase gene activation in response to stress.

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Section: Discussionmentioning

confidence: 97%

Hormonal and Stress Induction of the Gene Encoding Common Bean Acetyl-Coenzyme A Carboxylase

2006

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“…gma-MIR-Cand016 and gma-MIR-Cand017 also appear to regulate target genes involved in gene expression. The bZIP transcription factor (TF) seems to be the target of gma-MIR-Cand017; this TF negatively regulates nodulation (Nishimura et al, 2002) and is present in plant-defense responses (Dröge-Laser et al, 1997). Other transcription factors have been shown to be regulated by miRNAs during nodulation, such as MtHAP2, which is controlled by miR169; HD-ZIPIII, regulated by miR166; and NAC1, regulated by miR164; all were identified during nodulation in M. truncatula (Combier et al, 2006;Boualem et al, 2008;D'haeseleer et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Prediction of potential novel microRNAs in soybean when in symbiosis

Barros-Carvalho¹,

Paschoal²,

Marcelino-Guimarães³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. MicroRNAs (miRNAs) are small molecules, noncoding proteins that are involved in many biological processes, especially in plants; among these processes is nodulation in the legume. Biological nitrogen fixation is a key process, with critical importance to the soybean crop. This study aimed to identify the potential of novel miRNAs to act during the root nodulation process. We utilized a set of transcripts that were differentially expressed in soybean roots 10 days after inoculation with Bradyrhizobium japonicum, which were obtained in a previous study, and performed a set of computational analyses that led us to select new miRNAs potentially involved in nodulation. Among these analyses, the set of transcripts were submitted to an in silico annotation of noncoding RNAs, including a search of similarity against miRNA public databases, ab initio tools for miRNA identification, structural search against miRNA families, prediction of the secondary structure of miRNA precursors, and prediction of the sequences of mature miRNAs. Subsequently, we applied filter procedures based on miRNA selections described in the literature (e.g., free energy value). In the next step, a manual curation inspection of the annotation was performed and the top candidates were selected and used for prediction of potential target genes, which were later checked manually in the database of the soybean genome. This prediction led us to the identification of 9 potential new miRNAs; among these, 4 were conserved in other plants. Moreover, we predicted their target genes might play important roles in the regulation of nodulation.

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“…For example, phosphorylation of Gbox binding factor 1 from Arabidopsis or of H/G-box binding factor 1 in elicited soybean cells increases the DNA binding activities of these factors (16,17). On the other hand, phosphorylation of opaque 2 from maize causes the opposite effect (18).…”

Section: Discussionmentioning

confidence: 99%

Phosphorylation of the Parsley bZIP Transcription Factor CPRF2 Is Regulated by Light

Wellmer

Kircher

Rügner

et al. 1999

Journal of Biological Chemistry

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The analysis of the complex network of signal transduction chains has demonstrated the importance of transcription factor activities for the control of gene expression. To understand how transcription factor activities in plants are regulated in response to light, we analyzed the common plant regulatory factor 2 (CPRF2) from parsley (Petroselinum crispum L.) that interacts with promoter elements of light-regulated genes. Here, we demonstrate that CPRF2 is a phosphoprotein in vivo and that its phosphorylation state is rapidly increased in response to light. Phosphorylation in vitro as well as in vivo occurs primarily within the C-terminal half of the factor, and is caused by a cytosolic 40-kDa protein serine kinase. In contrast to other plant basic leucinezipper motif factors, phosphorylation of CPRF2 does not alter its DNA binding activity. Therefore, we discuss alternative functions of the light-dependent phosphorylation of CPRF2 including the regulation of its nucleocytoplasmic partitioning.Light is probably the most variable environmental factor controlling plant development. To monitor light quality and quantity, plants have evolved at least three different photoreceptor systems: the red/far-red reversible phytochromes, the blue/UV-A, and the UV-B photoreceptors (1). The most well understood of these photoreceptors is the phytochrome system (2, 3).Besides the search for appropriate mutants, other approaches have been used to understand the signal transduction mechanisms mediated by photoreceptors. (i) Characterizing the photoreceptors themselves and searching for interacting proteins, (ii) unraveling the role of signal mediators like Ca 2ϩ , calmodulin, cGMP, and phosphorylation events (4), and (iii) analyzing DNA-binding proteins that interact with promoter elements of light-regulated genes. As shown, for example, for chalcone synthase or chlorophyll a/b-binding protein genes, promoter elements that mediate light responsiveness frequently contain the palindromic DNA motif ACGT, that, depending on the adjacent nucleotides, is part of the so-called G-box (CACGTG) or C-box (GACGTC) sequences (5). However, G-and C-boxes are not only found in the promoters of lightregulated genes but also in promoters of genes that respond to other exogenic and endogenic stimuli such as stress, hormones, and cell cycle-related signals (6). Transcription factors containing a basic leucine-zipper motif (bZIP), 1 as, for example, the common plant regulatory factors (CPRFs) from parsley (7-11) and G-box binding factors from Arabidopsis (12-14), were shown to bind to the G-box or the C-box, respectively, in vitro as well as in vivo and form specific homo-and heterodimers (7,8,12,13). Since CPRF and G-box binding factors proteins, which have molecular masses between 35 and 45 kDa, are encoded by multigene families (15), it is difficult to define which and how many members directly act as transcription factors regulating a certain inducible gene.The regulation of the activities of these factors in response to light is poorly understood...

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Rapid stimulation of a soybean protein-serine kinase that phosphorylates a novel bZIP DNA-binding protein, G/HBF-1, during the induction of early transcription-dependent defenses

Cited by 169 publications

References 75 publications

Hormonal and Stress Induction of the Gene Encoding Common Bean Acetyl-Coenzyme A Carboxylase

Hormonal and Stress Induction of the Gene Encoding Common Bean Acetyl-Coenzyme A Carboxylase

Prediction of potential novel microRNAs in soybean when in symbiosis

Phosphorylation of the Parsley bZIP Transcription Factor CPRF2 Is Regulated by Light

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