Rapid sensitive fluorescence detection of cadmium (II) with pyrene excimer switching aptasensor

Yu, Hao; Zhao, Qiang

doi:10.1016/j.jes.2022.03.047

Cited by 12 publications

(7 citation statements)

References 33 publications

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“…The results indicated that introduction of PS modification at the specific site of the aptamer greatly enhanced the binding affinity of the aptamer, and improved its FA sensitivity to Cd 2+ , while the aptamer CBA15-G3S-T12-TMR enabled a lower detection limit. Compared with other aptamer-based methods for Cd 2+ detection [ 15 , 16 , 18 , 21 , 32 , 33 , 34 ], our method had better or comparable sensitivity ( Table S3 ). Although the detection limit of our FA sensor was higher than that of the reported method [ 35 ], our strategy is simple, rapid, and requires only one TMR-labeled aptamer.…”

Section: Resultsmentioning

confidence: 93%

“…To identify an appropriate position at the aptamer for TMR labeling, enabling the TMR-labeled aptamer to show sensitive FA response to Cd 2+ , we tested a series of sites of the 15-mer Cd 2+ -binding aptamer (CBA15), including 3′ end, internal thymine bases (5 T, 6 T, 12 T), and 5′ end ( Table 1 ). CBA15 was truncated from a 21-mer aptamer sequence against Cd 2+ [ 15 , 21 ]. We measured the FA responses of different TMR-labeled CBA15 to Cd 2+ in the binding buffer containing 20 mM Tris-HCl (pH 7.5) and 20 mM NaCl.…”

Section: Resultsmentioning

confidence: 99%

“…Aptamer-based sensors have enabled detection of various targets such as metal ions, small molecules, proteins, and cells, due to the appealing features of aptamers [ 11 , 12 , 13 , 14 ]. Since aptamers for Cd 2+ were identified [ 15 , 16 ], aptamer-based sensors and assays for Cd 2+ have become possible and have provided new approaches for detection of Cd 2+ , including electrochemistry, colorimetry, and fluorescent methods [ 15 , 16 , 17 , 18 , 19 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

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A Sensitive Aptamer Fluorescence Anisotropy Sensor for Cd2+ Using Affinity-Enhanced Aptamers with Phosphorothioate Modification

Zhao

2022

Biosensors

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Rapid and sensitive detection of heavy metal cadmium ions (Cd2+) is of great significance to food safety and environmental monitoring, as Cd2+ contamination and exposure cause serious health risk. In this study we demonstrated an aptamer-based fluorescence anisotropy (FA) sensor for Cd2+ with a single tetramethylrhodamine (TMR)-labeled 15-mer Cd2+ binding aptamer (CBA15), integrating the strengths of aptamers as affinity recognition elements for preparation, stability, and modification, and the advantages of FA for signaling in terms of sensitivity, simplicity, reproducibility, and high throughput. In this sensor, the Cd2+-binding-induced aptamer structure change provoked significant alteration of FA responses. To acquire better sensing performance, we further introduced single phosphorothioate (PS) modification of CBA15 at a specific phosphate backbone position, to enhance aptamer affinity by possible strong interaction between sulfur and Cd2+. The aptamer with PS modification at the third guanine (G) nucleotide (CBA15-G3S) had four times higher affinity than CBA15. Using as an aptamer probe CBA15-G3S with a TMR label at the 12th T, we achieved sensitive selective FA detection of Cd2+, with a detection limit of 6.1 nM Cd2+. This aptamer-based FA sensor works in a direct format for detection without need for labeling Cd2+, overcoming the limitations of traditional competitive immuno-FA assay using antibodies and fluorescently labeled Cd2+. This FA method enabled the detection of Cd2+ in real water samples, showing broad application potential.

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Section: Resultsmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Sensitive Aptamer Fluorescence Anisotropy Sensor for Cd2+ Using Affinity-Enhanced Aptamers with Phosphorothioate Modification

Zhao

2022

Biosensors

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“…This pyrene-labeled aptasensor shows selective sensing of cadmium ions and even detects Cd 2 + ions in tap water (Figure 16). [108] This pyrene-labeled fluorescence aptasensor is observed to be very promising for the rapid and sensitive detection of cadmium ions.…”

Section: Sensors For CD (Ii)mentioning

confidence: 99%

Pyrene‐Appended Luminescent Probes for Selective Detection of Toxic Heavy Metals and Live Cell Applications

Srivastava,

Mishra,

Pathak

et al. 2024

ChemistrySelect

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Heavy metal contamination has become a global environmental problem and currently drawn much attention from researchers in worldwide. High exposure to heavy metals can lead to problems like kidney, liver and brain damage, skin and lung cancer, etc. Rapid and sensitive detection of heavy metals is of immense importance in environmental monitoring. Over the past few decades, enormous efforts have been made to design various sensors to identify and monitor these harmful metal ions. In Comparison with other methods, fluorescence‐based chemosensors have received extensive attention because of the advantages of high sensitivity, low cost, real‐time monitoring, simple operation, etc. The pyrene derivatives are significant polycyclic aromatic hydrocarbons (PAHs) with strong fluorescence. They are widely used as fluorescent probes in many applications due to their easy modification, high fluorescence quantum yield, and strong fluorescence emission in live cells, outstanding cell permeability, and very low cytotoxicity. The SDG 6: Clean water and sanitation alarm a strong strategy, and careful identification of the risk connected to environmental pollutants needs urgent attention in this situation. Therefore, we highlight different types of pyrene‐based fluorophores and the fluorescence mechanisms for the sensitive and selective detection methodology of Arsenic, Mercury, Cadmium, Lead, and Chromium heavy metals.

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“…Sensing of transition metals, especially heavy metals, such as mercury, 13 chromium, 14 cadmium, 15 and arsenic, 16 among others, has been an actively studied area given the environmental toxicity exhibited by some of these metals. Copper is one such element (mainly in the Cu 2+ form) that has abundant natural occurrence in the environment and in the human body in the form of enzymes such as tyrosinase 17 (oxidase enzyme for controlling melamine production), orgotein 18 (a superoxide dismutase enzyme), ceruloplasmin 19 (carrier of copper in blood), and many others.…”

Section: Introductionmentioning

confidence: 99%

Phenothiazine-Based Cu(II)-Selective Fluorescent Sensor: GHK-Cu Sensing Applications

Sahu,

Yadav,

Gunturu

et al. 2023

J. Org. Chem.

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Sensing important metals in different environments is an important area and involves the development of a wide variety of metal-sensing materials. The employment of fluorescent sensors in metal sensing has been one of the most widely applied methodologies, and the identification of selective metal sensors is important. We herein report a phenothiazine-based Cu(II) fluorescent sensor that is highly selective to Cu(II) ions compared with other transition metal salts. The Lewis acidity of the Cu(II) salt certainly was found to be a factor for obtaining an enhanced sensing response in MeOH as the solvent, while a ratio of 1:1 was calculated to be the most optimum for getting the desired response.

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Rapid sensitive fluorescence detection of cadmium (II) with pyrene excimer switching aptasensor

Cited by 12 publications

References 33 publications

A Sensitive Aptamer Fluorescence Anisotropy Sensor for Cd2+ Using Affinity-Enhanced Aptamers with Phosphorothioate Modification

A Sensitive Aptamer Fluorescence Anisotropy Sensor for Cd2+ Using Affinity-Enhanced Aptamers with Phosphorothioate Modification

Pyrene‐Appended Luminescent Probes for Selective Detection of Toxic Heavy Metals and Live Cell Applications

Phenothiazine-Based Cu(II)-Selective Fluorescent Sensor: GHK-Cu Sensing Applications

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Rapid sensitive fluorescence detection of cadmium (II) with pyrene excimer switching aptasensor

Cited by 12 publications

References 33 publications

A Sensitive Aptamer Fluorescence Anisotropy Sensor for Cd2+ Using Affinity-Enhanced Aptamers with Phosphorothioate Modification

A Sensitive Aptamer Fluorescence Anisotropy Sensor for Cd2+ Using Affinity-Enhanced Aptamers with Phosphorothioate Modification

Pyrene‐Appended Luminescent Probes for Selective Detection of Toxic Heavy Metals and Live Cell Applications

Phenothiazine-Based Cu(II)-Selective Fluorescent Sensor: GHK-Cu Sensing Applications

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Rapid sensitive fluorescence detection of cadmium (II) with pyrene excimer switching aptasensor