1951
DOI: 10.1001/archderm.1951.01570030057007
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Rapid Permanent Staining and Mounting of Skin Scrapings and Hair

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Cited by 11 publications
(2 citation statements)
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“…Cotton blue staining has to be done in a 2-stcp procedure and did not sufficiently contrast the fungi. Methylene blue was mainly proposed for the permanent staining of skin scrapings and hair without clearing procedure [15,[28][29], Congo red is known to stain chitin, a main constituent of the cell wall of fungi. We therefore evaluated its appli cability for routine direct examination.…”
Section: Direct Examination With Staining O F Fungal Elementsmentioning
confidence: 99%
“…Cotton blue staining has to be done in a 2-stcp procedure and did not sufficiently contrast the fungi. Methylene blue was mainly proposed for the permanent staining of skin scrapings and hair without clearing procedure [15,[28][29], Congo red is known to stain chitin, a main constituent of the cell wall of fungi. We therefore evaluated its appli cability for routine direct examination.…”
Section: Direct Examination With Staining O F Fungal Elementsmentioning
confidence: 99%
“…Thus, dead cells would stain, living cells would remain colourless. [9][10][11][12] The PAS stain is a simple stain that demonstrates polysaccharides. Cellulose and chitin, two substances rich in polysaccharides, are found in the cell walls of fungi.…”
Section: Discussionmentioning
confidence: 99%