Rapid Monitoring of Vancomycin Concentration in Serum Using Europium (III) Chelate Nanoparticle-Based Lateral Flow Immunoassay

Abstract

Establishing personalized medication plans for patients to maximize therapeutic efficacy and minimize the toxicity of vancomycin (VAN) requires rapid, simple, and accurate monitoring of VAN concentration in body fluid. In this study, we have developed a simple and rapid analytical method by integrating Eu (III) chelate nanoparticles (CN-EUs) and lateral flow immunoassay (LFIA) to achieve the real-time monitoring of VAN concentration in serum within 15 min. This approach was performed on nitrocellulose (NC) membrane assembled LFIA strips via indirect competitive immunoassay and exhibited a wide linear range of detection (0.1–80 μg*ml−1) with a low limit of detection (69.2 ng*ml−1). The coefficients of variation (CV) of the intra- and inter-assay in the detection of VAN were 7.12–8.53% and 8.46–11.82%, respectively. The dilution test and specificity indicated this method had a stability that was not affected by the serum matrix and some other antibiotics. Furthermore, the applicability of the proposed method was assessed by comparing the determined results with those measured by LC-MS/MS, showing a satisfactory correlation (R2 = 0.9713). The proposed CN-EUs-based LFIA manifested promising analytical performance, which showed potential value in the real-time monitoring of VAN and could help optimize the clinical use of more antibiotics.

A simple strategy for low-cost portable monitoring of plasma drug concentrations using a sustainable boron-doped-diamond chip

“…To our knowledge, this performance is better than the turnaround time of other point-of-care biosensors (15−90 min). 38,39 Additional merits of the operating procedures are outlined below. Measurement accuracy was verified by means of rat and patient samples and a 'general' calibration curve (Figures 4e−h and 5).…”

A simple and rapid strategy for monitoring vancomycin concentration in serum using a multicolor immunosensor based on the ratio of gold nanobipyramids and a product of cetyltrimethylammonium bromide-blue oxide of 3,3′,5,5′-tetramethylbenzidine interaction

“…Vancomycin (VAN) is a glycopeptide antibiotic and the rstline antimicrobial agent in the treatment of methicillinresistant Staphylococcus aureus (MRSA) infections. [1][2][3] The rational use of VAN is highly important in clinical practice, but is challenging due to its toxicity and narrow therapeutic window. 4,5 High VAN concentrations can be adverse, causing the most serious side effects such as kidney injury, nephrotoxicity and ototoxicity.…”

“…In the era of precision medicine, rapidity and simplicity of drug concentration monitoring have become the trend of its clinical application. 2 Currently, the widely used methods for the TDM of VAN include high-performance liquid chromatography (HPLC), liquid chromatography-tandem mass spectrometry (LC-MS/MS), chemiluminescence, enzyme-linked immunosorbent assay (ELISA), uorescence polarization immunoassay (FPIA) and enzyme-multiplied immunoassay technique (EMIT). 1,[10][11][12] Although analytical methods such as HPLC and LC-MS/MS have obvious advantages of both sensitivity and accuracy, their applications rely on tedious pretreatment, trained personnel and expensive equipment.…”

“…Most notably, due to the time-consuming process of sample preparation, analysis procedures, and sample transportation (from the patient's location to the laboratory), VAN is catabolized in the patient, making the test results unsuitable as a reference for drug dose adjustment. 2,13 The above-mentioned limitations of the currently available approaches require a new detection technique to be proposed with a low cost, rapid and simple platform for the on-site TDM of VAN. In view of the advantages of low cost, strong practicality and simplicity, colorimetric immunosensors have been reported as powerful tools of rapid detection in many elds such as environment, medical diagnosis and food safety. [14][15][16][17] Colorimetric immunosensors could also become the preferable choice of VAN TDM due to their portability for rapid and on-site detection.…”

“…[14][15][16][17] Colorimetric immunosensors could also become the preferable choice of VAN TDM due to their portability for rapid and on-site detection. 2 However, single-color changes (from dark to light or from light to dark) show poor sensitivity for direct readouts. 18 Compared with the conventional single-color assay, the multicolor assay shows high resolution and accuracy for semiquantitative determination.…”