Rapid micropropagation of Curcuma longa using bud explants pre-cultured in thidiazuron-supplemented liquid medium

Prathanturarug, Sompop; Soonthornchareonnon, Noppamas; Chuakul, Wongsatit; Phaidee, Y.; Saralamp, Promchit

doi:10.1007/s11240-004-1020-x

Cited by 46 publications

(28 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results suggest that a short (6 h) exposure of explants to 25 or 50 lmol L -1 TDZ, prior to the transfer to SH multiplication medium with growth regulators, led to increased shoot proliferation of H. procumbens. Similar stimulating effects of TDZ pretreatment on multiple shoot formation have been previously reported for some other plant species, such as Curcuma longa (Prathanturarug et al 2003(Prathanturarug et al , 2005, Prunus cerasus (Song and Sink 2005), Ocimum basilicum (Siddique and Anis 2007), Rosa spp. (Kucharska and Orlikowska 2009) and Nyctanthes abor-tristis (Jahan et al 2011).…”

Section: Shoot Proliferationsupporting

confidence: 85%

“…The response of the explants in terms of a number of regenerated shoots and their length to different concentrations and duration of TDZ exposure was determined. TDZ (a synthetic phenylurea derivative) is efficient for the formation of adventitious shoots and axillary shoot proliferation, and the technique of explant pretreatment with the growth regulator has been successfully used by several authors for the improvement of in vitro plant micropropagation (Singh and Syamal 2001;Prathanturarug et al 2003Prathanturarug et al , 2005Siddique and Anis 2007;Jahan et al 2011). For example, Jahan et al (2011) used it to micropropagate Nyctanthes arbor-tristis.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Influence of thidiazuron (TDZ) pretreatment of shoot tips on shoot multiplication and ex vitro acclimatization of Harpagophytum procumbens

2014

View full text Add to dashboard Cite

The effects of thidiazuron (TDZ) pretreatment of shoot tips on Harpagophytum procumbens shoot proliferation and successive stages of micropropagation, i.e. rooting of regenerated shoots and acclimatization of plantlets to ex vitro conditions, were described in the present study. The best response in terms of shoot proliferation (about seven shoots/explant) and shoot length (3.2 ± 0.4 cm) was obtained when explants pretreated with 25 lmol L -1 TDZ for 6 h were cultured on Schenk and Hildebrandt medium containing indole-3-acetic acid (IAA) (0.57 lmol L -1 ) and 6-benzylaminopurine (BAP) (8 lmol L -1 ). Under these conditions, a 330 % increase in shoot multiplication over TDZ non-pretreatment culture was achieved and TDZ pretreatment shoots were longer compared to those in control culture (2.6 ± 0.3 cm). The TDZ pretreatment did not affect the percentage of rooted shoots, length of roots and number of roots formed per shoot. The rooted plantlets were transplanted from in vitro to pots with soil and grown during 1 year in the greenhouse. The hardening process was difficult and time-consuming. We found that the plants developed from the TDZ pretreated culture were superior to plants from non-pretreated culture in terms of survival rate and morphological features, such as shoot length, leaf size, flowering and earlier root tuberisation. Random amplified polymorphic DNA and inter-simple sequence repeat analyses of pretreatment with TDZ plants showed genetic similarity to non-pretreatment plants. We conclude that applying the strategy of initial explant pretreatment with TDZ may be valuable for the improvement in H. procumbens in vitro propagation.

show abstract

Section: Shoot Proliferationsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Influence of thidiazuron (TDZ) pretreatment of shoot tips on shoot multiplication and ex vitro acclimatization of Harpagophytum procumbens

2014

View full text Add to dashboard Cite

show abstract

“…A successful regeneration of the subdivided rosettes from TDZ was observed when they were transferred to MS culture media without growth regulators, showing that subcultivation in these conditions is crucial for later development of these explants (Khalafalla and Hattori, 1999;Joshi et al, 2008). Although Prathanturarug et al (2005) has suggest that a second treatment with TDZ may increase culture multiplication, the results from our work do not corroborate with this possibility, since explants regeneration in culture media supplemented with high doses of TDZ was very low. The plantlets regenerated in MS medium presented normal morphology and similarity of plants not subjected to TDZ treatment, a result also found by Siddique and Anis (2007) in Ocimum basilicum L. cultures.…”

Section: Cultured In Vitrocontrasting

confidence: 64%

An improved protocol for in vitro propagation of the medicinal plant Mimosa pudica L.

Bianchetti¹,

Resende²,

Pacheco³

et al. 2017

Afr. J. Biotechnol.

View full text Add to dashboard Cite

This work aimed to develop a protocol for the in vitro establishment, multiplication, rooting and ex vitro acclimatization of Mimosa pudica L., a species used in folk medicine and with pharmacological activity. Aseptic cultures were established from seeds inoculated in MS medium, without growth regulators, followed by an in vitro stabilization phase in culture medium supplemented with 2.22 μM BAP. The cultures were transferred to MS medium supplemented with different cytokinins, combined or not with an auxin, aiming its large-scale propagation. The culture medium supplemented with 5 µM BAP plus 0.5 µM NAA provided the highest multiplication rate and top quality plantlets. The combination of 0.6 µM TDZ plus 0.05 µM NAA resulted in higher multiplication rates than in response to combination of BAP plus NAA, although the subsequent maintenance of the cultures in a medium without growth regulators has resulted in low regenerative response. In vitro rooting of micro-cuttings was high even in the absence of auxins. Over 90% of plantlets transferred to the greenhouse survived after the acclimatization phase. Acclimatized plants presented normal vegetative and reproductive development. The procedures established in the present study allow a massive production of M. pudica plants for further pharmacological studies.

show abstract

“…Among the various tested concentrations and culturing durations, the highest shoot regeneration frequency (90%), mean number of shoots (23.1 § 2.15) and maximum shoot length (5.3 § 0.83 cm) were recorded after 8 weeks at 50 mmol/L TDZ for 8 days culture duration (Figure 2(A)). The effectiveness of short term exposure and stimulating effect of TDZ on bud break and multiple shoot formation has been reported earlier for several medicinal and aromatic plant species including Ocimum basilicum, [10] Arachis correntina, [65] Curcuma longa, [66] Curculigo orchioides [67] and Mentha arvensis. [68] It is suggested that TDZ may be needed as a trigger for initiating the proliferation of shoot meristems.…”

Section: Effect Of Tdz Dosage and Duration In Liquid Ms Mediummentioning

confidence: 74%

Thidiazuron induced efficientin vitromultiplication andex vitroconservation ofRauvolfia serpentina– a potent antihypertensive drug producing plant

Alatar

2015

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

An efficient system for in vitro propagation of the endangered medicinal plant Rauvolfia serpentina has been developed. Proliferation of shoots was achieved from nodal segment explants, excised from a field grown plants on Murashige and Skoog (MS) medium supplemented with thidiazuron (TDZ) (0.1À2.5 mmol/L) although with low regeneration response and few number of shoots per explant. Greater number of shoots was achieved from nodal explants pretreated with higher concentrations of TDZ (5À100 mmol/L) in liquid MS medium for different time periods (4, 8, 12 and 16 days), followed by their transfer on a growth regulator-free medium. The highest response in terms of percent regeneration (90%), average number of shoots/explant (23.17 § 2.15) and maximum shoot length (5.3 cm § 0.83 cm) were achieved by pretreating the nodal explants with 50 mmol/L TDZ for 8 days. Rooting was achieved in MS medium supplemented with 0.5 mmol/L indole-3-butyric acid with an average of 5.50 § 0.92 roots per microshoot. The rooted plantlets were transferred to soil, where 90% of them grew well and exhibited a normal development. Chlorophyll a and b, carotenoids, net photosynthetic rate and antioxidative enzymes, viz., superoxide dismutase and catalase activities were measured in leaf tissues during ex vitro acclimatization at 0, 7, 14, 21 and 28 days. Most of the activities showed a decreasing trend but subsequently increased after 7 days of acclimatization. These changes suggested the adjustment of the in vitro propagated plantlets to ex vitro conditions.

show abstract

Rapid micropropagation of Curcuma longa using bud explants pre-cultured in thidiazuron-supplemented liquid medium

Cited by 46 publications

References 10 publications

Influence of thidiazuron (TDZ) pretreatment of shoot tips on shoot multiplication and ex vitro acclimatization of Harpagophytum procumbens

Influence of thidiazuron (TDZ) pretreatment of shoot tips on shoot multiplication and ex vitro acclimatization of Harpagophytum procumbens

An improved protocol for in vitro propagation of the medicinal plant Mimosa pudica L.

Thidiazuron induced efficientin vitromultiplication andex vitroconservation ofRauvolfia serpentina– a potent antihypertensive drug producing plant

Contact Info

Product

Resources

About