Rapid method for separation and purification of four isoenzymes of phosphodiesterase from trimeresurus flavoviridis (habu snake) venom

The Sequence and a Three-Dimensional Structural Analysis Reveal Substrate Specificity among Snake Venom Phosphodiesterases

“…SVPDEs are metalloenzymes that contain zinc and calcium ions [6,26,35,36,67]. The zinc ion participates in the active site and is important for catalytic activity [26,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65,66,67]. In the modeled structure of PDE_ Ca , two zinc ions and one calcium ion were found (Figure 4).…”

“…PDEs have been isolated from various snake venoms, including Deinagkistrodon acutus [19], Bothrops atrox [20], Bothrops alternatus [21], Cerastes vipera [22], Crotalus atrox [23], Crotalus adamanteus [24,25] Crotalus durissus terrificus [25], Protobothrops flavoviridis [26], and Vipera aspis [27]. In addition to snake venoms, they also occur in spider venoms [28].…”

The Sequence and a Three-Dimensional Structural Analysis Reveal Substrate Specificity among Snake Venom Phosphodiesterases

“…SVPDEs are metalloenzymes that contain zinc and calcium ions [6,26,35,36,67]. The zinc ion participates in the active site and is important for catalytic activity [26,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65,66,67]. In the modeled structure of PDE_ Ca , two zinc ions and one calcium ion were found (Figure 4).…”

“…PDEs have been isolated from various snake venoms, including Deinagkistrodon acutus [19], Bothrops atrox [20], Bothrops alternatus [21], Cerastes vipera [22], Crotalus atrox [23], Crotalus adamanteus [24,25] Crotalus durissus terrificus [25], Protobothrops flavoviridis [26], and Vipera aspis [27]. In addition to snake venoms, they also occur in spider venoms [28].…”

The Sequence and a Three-Dimensional Structural Analysis Reveal Substrate Specificity among Snake Venom Phosphodiesterases

“…SVPDEs are metalloenzymes that contain zinc and calcium ions [6,26,35,36,67]. The zinc ion participates in the active site and is important for catalytic activity [26,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65,66,67]. In the modeled structure of PDE_ Ca , two zinc ions and one calcium ion were found (Figure 4).…”

“…PDEs have been isolated from various snake venoms, including Deinagkistrodon acutus [19], Bothrops atrox [20], Bothrops alternatus [21], Cerastes vipera [22], Crotalus atrox [23], Crotalus adamanteus [24,25] Crotalus durissus terrificus [25], Protobothrops flavoviridis [26], and Vipera aspis [27]. In addition to snake venoms, they also occur in spider venoms [28].…”

The Sequence and a Three-Dimensional Structural Analysis Reveal Substrate Specificity among Snake Venom Phosphodiesterases

“…SVPDEs are metalloenzymes that contain zinc and calcium ions [6,26,35,36,67]. The zinc ion participates in the active site and is important for catalytic activity [26,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65,66,67]. In the modeled structure of PDE_ Ca , two zinc ions and one calcium ion were found (Figure 4).…”

“…PDEs have been isolated from various snake venoms, including Deinagkistrodon acutus [19], Bothrops atrox [20], Bothrops alternatus [21], Cerastes vipera [22], Crotalus atrox [23], Crotalus adamanteus [24,25] Crotalus durissus terrificus [25], Protobothrops flavoviridis [26], and Vipera aspis [27]. In addition to snake venoms, they also occur in spider venoms [28].…”