Rapid measurement of glomerular filtration rate in small animals

Ido, Yasuo; Tilton, Ronald G.; Chang, Katherine; Pugliese, Giuseppe; Williamson, Joseph R.

doi:10.1038/ki.1992.60

Cited by 2 publications

(2 citation statements)

References 14 publications

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“…The need for cannulae and restraint can be avoided by the use of implanted osmotic minipumps for delivery of inulin (Shirley et al 1989); however, the pumps will not function for more than 4 weeks. In order to circumvent some of the problems associated with the measurement of renal clearance, methods have been introduced in which GFR is calculated from the plasma disappearance of a glomerular marker, such as 51 Cr‐EDTA or 57 Co‐EDTA, following a bolus intravenous injection (Seefeldt & Houghton, 1990; Ido et al 1992). In a subsequent development, Nankivell et al (1992) used 99m Tc‐diethylenetriamene pentaacetic (DTPA) and injected the bolus dose intraperitoneally, thereby obviating the need for an intravenous cannula.…”

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confidence: 99%

Time course of the renal functional response to partial nephrectomy: measurements in conscious rats

Chamberlain

Shirley

2007

Experimental Physiology

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Previous investigations into the functional responses of the surviving nephrons following reductions in renal mass have been performed largely in anaesthetized animals and have taken little account of how the compensatory changes develop with time. The present study has assessed a method for determining glomerular filtration rate (GFR) in unrestrained, uncatheterized, conscious rats (plasma disappearance of 99m Tc-diethylenetriamene pentaacetic acid (DTPA)) and has used this method to document the time course of the changes in GFR over a 32 day period following uninephrectomy or 5/6 nephrectomy. Concurrent measurements of excretion rates and of the clearance of lithium (the latter being an index of end-proximal fluid delivery) provided information on changes in overall tubular function and segmental reabsorption. After uninephrectomy, the GFR of the remaining kidney (compared with that of a single kidney of sham-operated animals) increased maximally (by ∼50%) within 8 days; after 5/6 nephrectomy, the increase in the GFR of the remnant kidney was maximal (at ∼300%) within 16 days. Overall excretion rates of sodium and potassium were well maintained in partially nephrectomized animals throughout the period of study, while the excretion of water increased (by ∼30% after uninephrectomy and by ∼120% after 5/6 nephrectomy), partly as a result of the compensatory increases in GFR but mainly as a consequence of moderate (after uninephrectomy) or marked (after 5/6 nephrectomy) reductions in fractional reabsorption. During the early period after 5/6 nephrectomy, potassium excretion sometimes exceeded the filtered load, indicating net secretion. Lithium clearance data indicated that the changes in tubular function after 5/6 nephrectomy include a reduction in fractional reabsorption in the proximal tubule, whereas after uninephrectomy any such effect on the proximal tubule is minor and transient.

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confidence: 99%

Time course of the renal functional response to partial nephrectomy: measurements in conscious rats

Chamberlain

Shirley

2007

Experimental Physiology

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“…At that time, his laboratory had just developed a method to assess albumin vascular permeability and regional blood flow using radio‐isotopes. There, I developed the mathematical basis for the measurement of vascular permeability and glomerular filtration rate, allowing us to more clearly monitor the nature of vascular dysfunction associated with diabetes or high glucose. In addition to using streptozotocin‐injected rats (hereafter referred to as STZ diabetic rats), his laboratory also developed a granulation tissue chamber model.…”

Section: Polyol Pathway and The Pseudo‐hypoxia Hypothesismentioning

confidence: 99%

Diabetic complications within the context of aging: Nicotinamide adenine dinucleotide redox, insulin C‐peptide, sirtuin 1–liver kinase B1–adenosine monophosphate‐activated protein kinase positive feedback and forkhead box O3

Ido

2016

J of Diabetes Invest

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Recent research in nutritional control of aging suggests that cytosolic increases in the reduced form of nicotinamide adenine dinucleotide and decreasing nicotinamide adenine dinucleotide metabolism plays a central role in controlling the longevity gene products sirtuin 1 (SIRT1), adenosine monophosphate‐activated protein kinase (AMPK) and forkhead box O3 (FOXO3). High nutrition conditions, such as the diabetic milieu, increase the ratio of reduced to oxidized forms of cytosolic nicotinamide adenine dinucleotide through cascades including the polyol pathway. This redox change is associated with insulin resistance and the development of diabetic complications, and might be counteracted by insulin C‐peptide. My research and others' suggest that the SIRT1–liver kinase B1–AMPK cascade creates positive feedback through nicotinamide adenine dinucleotide synthesis to help cells cope with metabolic stress. SIRT1 and AMPK can upregulate liver kinase B1 and FOXO3, key factors that help residential stem cells cope with oxidative stress. FOXO3 directly changes epigenetics around transcription start sites, maintaining the health of stem cells. ‘Diabetic memory’ is likely a result of epigenetic changes caused by high nutritional conditions, which disturb the quiescent state of residential stem cells and impair tissue repair. This could be prevented by restoring SIRT1–AMPK positive feedback through activating FOXO3.

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Rapid measurement of glomerular filtration rate in small animals

Cited by 2 publications

References 14 publications

Time course of the renal functional response to partial nephrectomy: measurements in conscious rats

Time course of the renal functional response to partial nephrectomy: measurements in conscious rats

Diabetic complications within the context of aging: Nicotinamide adenine dinucleotide redox, insulin C‐peptide, sirtuin 1–liver kinase B1–adenosine monophosphate‐activated protein kinase positive feedback and forkhead box O3

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