2005
DOI: 10.1002/lt.20446
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Abstract: We have developed a novel bioreactor based on the observation that isolated porcine hepatocytes rapidly and spontaneously aggregate into spheroids under oscillation conditions. The purpose of this study was to characterize the influence of oscillation frequency (0.125 Hz, 0.25 Hz), cell density (1-10 ؋ 10 6 cells/mL), and storage condition (fresh, cryopreserved) of porcine hepatocytes on the kinetics of spheroid formation. The viability and metabolic performance of spheroid hepatocytes was also compared to mon… Show more

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Cited by 130 publications
(124 citation statements)
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“…Agitation frequency or spinner velocity, as well as cell density, are significant variables in this method of spheroid formation. As reported by Santo et al [42] and Nyberg et al [46] as agitation frequency increased, smaller spheroids were generated. However, the agitation or stirring rate must be kept above a specific value to hinder cell sedimentation during the spheroid formation process.…”
Section: Bioreactor Flasksmentioning
confidence: 51%
“…Agitation frequency or spinner velocity, as well as cell density, are significant variables in this method of spheroid formation. As reported by Santo et al [42] and Nyberg et al [46] as agitation frequency increased, smaller spheroids were generated. However, the agitation or stirring rate must be kept above a specific value to hinder cell sedimentation during the spheroid formation process.…”
Section: Bioreactor Flasksmentioning
confidence: 51%
“…Our BAL device contains 1.0×10 10 hepatocytes to meet the need of patients with ALF. The liver of an adult canine weighs about 335 kg and contains 3.35×10 9 hepatocytes. Therefore, the BAL device could be used in this experiment.…”
Section: Discussionmentioning
confidence: 99%
“…The long-term maintenance of hepatocyte function is crucial for any BAL system. Some systems use hepatocytes attached to microcarrier beads or multicellular spheroid aggregates [9][10][11] . In regard to bioreactor design, various bioreactor configurations have been proposed that employ glass plates, hollow fiber membranes, encapsulation in biological matrices, and 3-dimensional carrier materials [12][13][14][15] .…”
Section: Introductionmentioning
confidence: 99%
“…MCAs have found applications in bio-artificial liver-assisted devices [7], organ printing [8], cartilage regeneration [9], wound healing [10], tumour modelling [11] and drug discovery [12]. MCAs can be formed in either stationary culture on special substrata, such as the non-adherent or positively-charged surfaces [13,14], ligand-coated surfaces [15], and thermo-responsive polymeric surfaces [16]; or in suspension culture using orbital shaker [9], spinner flasks [17], rotating wall vessels [18], and hanging drops [19].…”
Section: Introductionmentioning
confidence: 99%
“…It is therefore critical to develop inter-cellular linkers with high degrees of consistency, economy and performance that facilitate rapid MCA formation for large-scale applications [7][8][9][10][11][12]. The effective linker concentration for MCA formation must be low enough to minimize cytotoxicity; the synthesis process must be simple and consistent for cost and quality controls.…”
Section: Introductionmentioning
confidence: 99%