Rapid Isolation and Identification of Pneumonia-Associated Pathogens from Sputum Samples Combining an Innovative Sample Preparation Strategy and Array-Based Detection

Pahlow, Susanne; Lehniger, Lydia; Hentschel, Stefanie; Seise, Barbara; Ehricht, Ralf; Berg, Albrecht; Popp, Jürgen; Weber, Karina

doi:10.1021/acsomega.9b00904

Cited by 5 publications

(2 citation statements)

References 28 publications

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“…The data demonstrate that the most predominant bacterial species isolated was S. pneumonia among patients in Zakho hospital. This agreed with the results of Al-Hadrawi et al (2019) and Pahlow et al (2019), who found that the higher rate of isolated bacteria was S. pneumonia in sputum and urine.…”

Section: Discussionsupporting

confidence: 93%

Isolation and identification of pathogenic bacterial species from public hospital patients at Zakho and Duhok cities

Allu, M. A.,

Yaseen, F. D.,

Khathayer, F. H.

et al. 2023

MJM

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Aims: This study aimed to isolate and identify different types of Gram-positive and Gram-negative bacteria from patients admitted to the general hospital of Duhok and Zakho cities. Isolates were identified based on standard microbiological and biochemical methods of bacterial identification. Methodology and results: In total, 2922 urine, throat swab and sputum samples (1941 females and 981 males) were collected and investigated. The findings indicated that Staphylococcus pneumonia and Staphylococcus aureus were prevalent among pathogens isolated from patients admitted to the general hospital of Zakho and Duhok cities, respectively. At Zakho hospital, the analysis of samples revealed that the rate of Streptococcus pneumonia isolation in urine samples was 15.83% for males and 22.92% for females. Regarding throat swabs, the rate was 5.43% for males and 4.87% for females. In terms of sputum, the rate of S. aureus isolation was 3.78% for males and 4.10% for females. On the other hand, in Duhok City, the results showed that the isolation rate of S. aureus was 20.25%, 3.58% and 4.83% in males for urine, sputum and throat swabs, respectively. The isolation rate of S. aureus 19.23%, 3.58% and 4.92% in females for urine, sputum and throat swabs, respectively. Moreover, the study highlighted the resistance of selected bacteria to different antibiotics, with Pseudomonas aeruginosa being the most resistant and Escherichia coli being the most sensitive. Conclusion, significance and impact of study:The study highlights the prevalence of S. pneumonia and S. aureus in Zakho and Duhok cities. Gender-based differences were observed in the S. pneumonia rates in urine samples of patients from Zakho hospital. The findings of this study revealed antibiotic resistance in bacterial isolates, with P. aeruginosa being the most resistant and E. coli was the most sensitive. These findings can assist in forming more effective management strategies for patients with bacterial infections.

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Section: Discussionsupporting

confidence: 93%

Isolation and identification of pathogenic bacterial species from public hospital patients at Zakho and Duhok cities

Allu, M. A.,

Yaseen, F. D.,

Khathayer, F. H.

et al. 2023

MJM

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“…So far, much research has been done on infectious agents, and microarrays of some species have been designed. Multiplex PCR along with amplified PCR hybridization linked to specific probes in a microarray chip, is a multiparametric technology to diagnose bacteria causing the infection (104,108) . The Respiratory Multiplex Array is used to detect 22 respiratory pathogens simultaneously in people suffering from the respiratory tract infection.…”

Section: Pcr Microarray Technologymentioning

confidence: 99%

A Review on Bacterial Respiratory Infections and Optimization of Their Identification

Mirhoseini

Heydari

Abednezhad

et al. 2020

IEM

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Background: The current narrative review aims to describe microbial agents causing pneumonia briefly. In addition, the ongoing review tries to introduce the diagnostic methods from biochemical to molecular tests used routinely and the promising molecular methods which will be used in near future. Materials & Methods: PubMed was searched for all review and original articles related to the lung infection. Studies providing insights into clinical symptoms, microbiology, risk factors, and diagnosis were included. Conclusion: Untreated respiratory infections are one of the most common health care problems worldwide. We tried to provide a collective view of new aspects of bacteriology and diagnosis methodology of lung infection detection.

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Mercury-tolerant metalophiles: A bio tool for remediation of mercury (Hg) affected Environs

Uqab,

Nazir,

Ganai

et al. 2024

Process Safety and Environmental Protection

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Rapid Isolation and Identification of Pneumonia-Associated Pathogens from Sputum Samples Combining an Innovative Sample Preparation Strategy and Array-Based Detection

Cited by 5 publications

References 28 publications

Isolation and identification of pathogenic bacterial species from public hospital patients at Zakho and Duhok cities

Isolation and identification of pathogenic bacterial species from public hospital patients at Zakho and Duhok cities

A Review on Bacterial Respiratory Infections and Optimization of Their Identification

Mercury-tolerant metalophiles: A bio tool for remediation of mercury (Hg) affected Environs

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