Rapid inactivation of vaccinia virus in suspension and dried on surfaces

Ferrier, Audrey; Garín, Daniel; Crance, J.M.

doi:10.1016/j.jhin.2004.01.012

Cited by 29 publications

(20 citation statements)

References 16 publications

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“…Although the effective concentrations will depend on the starting amounts of virus, making comparisons difficult, it is interesting to note that the effective 1-propanol concentrations required to inactivate VHSV were similar to those determined by others to inactivate VHSV in suspension (Kurita et al 2002). The similarity between surface and suspension tests need not always be the case because adsorbed viruses might be more or less susceptible than viruses in suspension (Bellamy 1995;Ferrier et al 2004;Gerba 1984). VHSV is more sensitive to inactivation by 1-propanol than CSV.…”

Section: Disinfection Of Absorbed Virussupporting

confidence: 54%

“…The influence of proteins on disinfection is an example of a problem that can be investigated by our system. Protein amount is one of the main factors affecting the efficacy of disinfectants (Ferrier et al 2004). The type and amount of protein adsorbed with the virus and/or in solution with the disinfectant could be varied easily.…”

Section: Disinfection Of Absorbed Virusmentioning

confidence: 99%

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Using 96-well tissue culture polystyrene plates and a fluorescence plate reader as tools to study the survival and inactivation of viruses on surfaces

2011

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A method for studying the behavior of viruses on surfaces has been developed and is illustrated by determining the temperatures that inactivate adsorbed viral hemorrhagic septicemia virus (VHSV) and the concentration of 1-propanol that disinfected surfaces with adsorbed VHSV and chum salmon virus (CSV). VHSV is a rhabdovirus; CSV, a reovirus, and they were detected with two fish cell lines, EPC and CHSE-214, respectively. When polystyrene tissue culture surfaces were incubated with virus, rinsed, and left to dry, they still supported the attachment and spreading of cell lines and after 7 days these cells showed the characteristic CPE of the viruses. Thus cells appeared to be infected directly from surfaces on which viruses had been adsorbed. Applying this property to 96-well plates allowed duplicate surfaces to be examined for their infectiousness or support of CPE. For each treatment 80 replicate surfaces in a 96-well plate were tested at one time and the results expressed as the number of wells showing CPE. VHSV adsorbed to polystyrene was inactivated by drying in the dark at temperatures above 14°C, but remained infectious for at least 15 days of drying at 4°C. For chemical sterilization of polystyrene surfaces with adsorbed virus, disinfection was achieved with 1-propanol at 40% for VHSV and at 60% for CSV. As CPE can be conveniently monitored in 96-well plates with a fluorescence plate reader, this method can be used to rapidly evaluate a variety of treatments for their ability to inactivate surface-bound viruses.

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Section: Disinfection Of Absorbed Virussupporting

confidence: 54%

Section: Disinfection Of Absorbed Virusmentioning

confidence: 99%

Using 96-well tissue culture polystyrene plates and a fluorescence plate reader as tools to study the survival and inactivation of viruses on surfaces

2011

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“…Our results confirm that the inactivation of viruses bound onto surfaces (carrier test) is more difficult than the reduction of viral titres in suspensions (Ferrier et al, 2004). In some cases, the value of inactivation in suspension was, however, limited by a high cytotoxicity and did not allow a direct comparison with the carrier test (Table 3 and 4).…”

Section: Discussionsupporting

confidence: 60%

“…The suspension tests are generally easier to perform and they are suitable for the evaluation of the basic activity of disinfectants (Sattar and Springthorpe, 2001). Since pathogens are normally found adsorbed to surfaces and embedded in organic debris, however, the results of carrier tests are more relevant for predicting the activity of chemical germicides in practice (Sattar et al, 2003;Ferrier et al, 2004).…”

mentioning

confidence: 99%

Comparative inactivation of Aujeszky's disease virus, Porcine teschovirus and Vesicular stomatitis I by chemical disinfectants

Dvořáková¹,

Prodělalová²,

Reichelova³

2008

Vet. Med. - Czech

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ABSTRACT:We tested the germicide activity of 1% Chloramin BM, 1% Incidin Plus, 1% Lysoformin 3000, 0.2% Mikasept KP, and 2% Sekusept Forte against viruses in suspension (suspension test) and dried onto a surface (carrier test). The agents of the porcine encephalomyelitis (Porcine teschovirus, strains CAPM V-86, CAPM V-37), Aujeszky's disease (strains CAPM V-166, CAPM V-327) and vesicular stomatitis (strains CAPM V-499, CAPM V-331) were used as model viruses. After 30 min contact time in both the suspension and carrier tests, the Porcine teschovirus was 4 lg inactivated only by Mikasept, which was thus the only disinfectant to meet the standard. The other disinfectants decreased the viral titre insufficiently. Under the same conditions, Aujeszky's disease virus was inactivated by at least 4 lg by all the tested disinfectants except for Chloramin BM which decreased the titre of CAPM V-166 only by 3.75 lg in the carrier test. For the inactivation of Vesicular stomatitis virus Chloramin BM and Mikasept KP were tested. Both the disinfectants reliably decreased the viral titre in both the suspension and carrier tests. Our results show that the inactivation of a surface-bound virus is more difficult than its inactivation in suspension. We confirm the high resistance of non-enveloped viruses (Porcine teschovirus) to chemical inactivation.

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“…A number of previous studies have shown that a higher organic load could result in a decreased effectiveness of viral inactivation (15,25,39). In the surface disinfection test, X174 and B40-8 suspensions were mixed with bovine serum albumin as an interfering substance to simulate clean (0.3% BSA) and dirty (3% BSA) conditions (12).…”

Section: Discussionmentioning

confidence: 99%

Inactivation of Murine Norovirus 1, Coliphage φX174, and Bacillus fragilis Phage B40-8 on Surfaces and Fresh-Cut Iceberg Lettuce by Hydrogen Peroxide and UV Light

Baert

Jonghe

et al. 2011

Appl Environ Microbiol

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Rapid inactivation of vaccinia virus in suspension and dried on surfaces

Cited by 29 publications

References 16 publications

Using 96-well tissue culture polystyrene plates and a fluorescence plate reader as tools to study the survival and inactivation of viruses on surfaces

Using 96-well tissue culture polystyrene plates and a fluorescence plate reader as tools to study the survival and inactivation of viruses on surfaces

Comparative inactivation of Aujeszky's disease virus, Porcine teschovirus and Vesicular stomatitis I by chemical disinfectants

Inactivation of Murine Norovirus 1, Coliphage φX174, and Bacillus fragilis Phage B40-8 on Surfaces and Fresh-Cut Iceberg Lettuce by Hydrogen Peroxide and UV Light

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