Rapid in situ assessmetn of physiological activities in bacterial biofilms using fluorescent probes

Yu, Feipeng Philip; McFeters, Gordon A.

doi:10.1016/0167-7012(94)90058-2

Cited by 90 publications

(57 citation statements)

References 26 publications

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“…Plate counts were an average of three times higher than epi uorescent estimates of CTC-positive bacteria (Table 1). A similar scenario has been observed in tetrazolium-stained samples of aquatic microorganisms Rodriguez et al 1992;Schaule et al 1993;Yu and McFeters 1994). Although tetrazolium reduction has been reported to accurately detect dehydrogenase activity and thus viability in aquatic and terrestrial environments (Awong et al 1985;Rodriguez et al 1992;Yu and McFeters 1994;Yu et al 1995), it did not consistently detect all metabolically competent and culturable E. coli and M. luteus.…”

Section: Comparison Of Active Cell Counts and Plate Countsmentioning

confidence: 86%

“…A similar scenario has been observed in tetrazolium-stained samples of aquatic microorganisms Rodriguez et al 1992;Schaule et al 1993;Yu and McFeters 1994). Although tetrazolium reduction has been reported to accurately detect dehydrogenase activity and thus viability in aquatic and terrestrial environments (Awong et al 1985;Rodriguez et al 1992;Yu and McFeters 1994;Yu et al 1995), it did not consistently detect all metabolically competent and culturable E. coli and M. luteus. As judged by tetrazolium reduction, cell respiration had ceased; however, as indicated by standard plate counts, some aerosolized microorganisms had not permanently lost repair mechanisms or respiration and growth capabilities.…”

Section: Comparison Of Active Cell Counts and Plate Countsmentioning

confidence: 86%

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A Combined Fluorochrome Method for Quantitation of Metabolically Active and Inactive Airborne Bacteria

Hernandez

1999

Aerosol Science and Technology

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ABSTRACT. To better understand the ecology of microorganisms in the environment and to quantify the concentrations of airborne microorganisms, methods are needed to count all microbial cells that are present and differentiate between those that are metabolically competent and those that are nonviable as they exist in situ. We developed and tested a direct epi uorescent method to estimate the quantity and activity of airborne bacteria aerosolized into full-scale rooms. Midget impingers, lled with the uorescing redox dye 5-cyano-2, 3-ditolyl tetrazolium chloride (CTC), were used to capture and stain metabolically active bacteria from room air. Both active and inactive bacteria were counterstained with a uorescein derivative and counted with an epi uorescent microscope. The choice of uorochrome stains allowed concurrent identi cation of active and inactive bacteria in the same microscopic eld. Airborne bacterial numbers determined by epi uorescence microscopy were compared to standard, nonselective colony counts cultured from midget impingers operated under identical conditions. Direct epi uorescent estimates of total airborne bacteria were higher than concurrent plate counts. However, estimates of active airborne bacteria were lower than concurrent plate counts. Variability experiments showed that the direct-count method was repeatable to within 35%.

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Section: Comparison Of Active Cell Counts and Plate Countsmentioning

confidence: 86%

Section: Comparison Of Active Cell Counts and Plate Countsmentioning

confidence: 86%

A Combined Fluorochrome Method for Quantitation of Metabolically Active and Inactive Airborne Bacteria

Hernandez

1999

Aerosol Science and Technology

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“…The abundance of these active bacteria is often correlated with total bacterial production (Lovejoy et al 1996, del Giorgio et al 1997 or with cell growth (Choi et al 1996, and the percentage of active bacteria is greater on particles than in free-living communities (B.F. , Yager et al 2001, Sherr et al 2002. Finally, the CTC technique has also been found appropriate for assessing the physiological status of benthic (Proctor & Souza 2001, Haglund et al 2002, groundwater (Hatzinger et al 2003), biofilm (Yu & McFeters 1994, Haglund et al 2002 and soil (Winding et al 1994, Whiteley et al 2003 bacteria.…”

Section: Abstract: Ctc · Syto13 · Flow Cytometry · Cell Sorting · Bamentioning

confidence: 99%

Cytometric evidence reconciling the toxicity and usefulness of CTC as a marker of bacterial activity

Gasol¹,

Arı́stegui²

2007

Aquat. Microb. Ecol.

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“…The biofilm image is shown in Figure 4. The biofilm reduced 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) into water insoluble crystalline CTCformazan, would be visualized as red crystals inside the membrane bound enzymes (19). This represents the presence of bacteria using electron-transport for energy generation, i.e., respiration.…”

Section: Confocal Microscopymentioning

confidence: 99%

Electrical Stress-Directed Evolution of Biocatalyst Texcoco Soil Community for Microbial Fuel Cell

et al. 2011

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Anode-respiring bacteria (ARB) perform an unusual form of respiration in which their electron acceptor is a solid anode. The focus of this study was to characterize the electrical stress direct evolution of biocatalysts as a way of enriching the community with ARB for microbial fuel cell. We gave the electrical stress continually to the Texcoco bacterial community at -150mV/SCE. The 4th day current started to increase and attained the maximum current of 0.35mA in the 15 th day. The current in this period was associated to biofilm growth. On the 15thday and by using cyclic voltammetery, an irreversible electron transfer reaction of alkaliphilic cytochrome was found, due to the electrode fouling. From the impedance measurement, the biofilm ARB resistance was determined (~250Ω). Further confocal microscopy studies of biofilm ARB revealed ~6µm thickness. In the single chamber microbial fuel cell, the electrochemical stressed biofilm-ARB exhibited a maximum power density of 79mW/m2

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Rapid in situ assessmetn of physiological activities in bacterial biofilms using fluorescent probes

Cited by 90 publications

References 26 publications

A Combined Fluorochrome Method for Quantitation of Metabolically Active and Inactive Airborne Bacteria

A Combined Fluorochrome Method for Quantitation of Metabolically Active and Inactive Airborne Bacteria

Cytometric evidence reconciling the toxicity and usefulness of CTC as a marker of bacterial activity

Electrical Stress-Directed Evolution of Biocatalyst Texcoco Soil Community for Microbial Fuel Cell

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