Rapid Imaging of Unsaturated Lipids at an Isomeric Level Achieved by Controllable Oxidation

Zhang, Jian; Huo, Xinming; Guo, Changjie; Ma, Xiaoxiao; Huang, Hanxi; Wang, Xiaohao; Tang, Fei

doi:10.1021/acs.analchem.0c03888

Cited by 18 publications

(19 citation statements)

References 33 publications

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“…In a recent study, Zhang et al developed an AFADESI method to study oxidative reactions to detect C = C double-bond isomers of unsaturated lipids. 8 The technique involved a short period of accelerated oxidation of mouse lung cancer tissues, following by rapid imaging. The ratio of two isomers was found to be reduced in tissue regions containing a concentration of cancer cells, demonstrating the ability to image the distributional difference in lipid isomers across healthy and cancerous tissues.…”

Section: Biological Materialsmentioning

confidence: 99%

Applications of ambient ionization mass spectrometry in 2021: An annual review

Rankin‐Turner

Reynolds

Turner

et al. 2022

Analytical Science Advances

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Ambient ionization mass spectrometry (AIMS) has revolutionized the field of analytical chemistry, enabling the rapid, direct analysis of samples in their native state. Since the inception of AIMS almost 20 years ago, the analytical community has driven the further development of this suite of techniques, motivated by the plentiful advantages offered in addition to traditional mass spectrometry. Workflows can be simplified through the elimination of sample preparation, analysis times can be significantly reduced and analysis remote from the traditional laboratory space has become a real possibility. As such, the interest in AIMS has rapidly spread through analytical communities worldwide, and AIMS techniques are increasingly being integrated with standard laboratory operations. This annual review covers applications of AIMS techniques throughout 2021, with a specific focus on AIMS applications in a number of key fields of research including disease diagnostics, forensics and security, food safety testing and environmental sciences. While some new techniques are introduced, the focus in AIMS research is increasingly shifting from the development of novel techniques toward efforts to improve existing AIMS techniques, particularly in terms of reproducibility, quantification and ease-of-use.

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Section: Biological Materialsmentioning

confidence: 99%

Applications of ambient ionization mass spectrometry in 2021: An annual review

Rankin‐Turner

Reynolds

Turner

et al. 2022

Analytical Science Advances

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show abstract

“…However, the discovery by Ma and Xia [114] that light-induced Paternò-Büchi (PB) reactions can aid DB position assignments with relative ease and with inexpensive equipment has sparked the interest in chemical derivatization strategies that enable structure elucidation. In particular, most recent derivatization strategies target lipid DBs or adjacent carbon atoms and are based on PB [114][115][116][117][118][119][120][121][122], epoxidation [123][124][125], ozonolysis [126], or hydroxylation reactions [127] (Scheme 2).…”

Section: Chemical Derivatization Prior To Tandem Msmentioning

confidence: 99%

Advanced tandem mass spectrometry in metabolomics and lipidomics—methods and applications

Heiles

2021

Anal Bioanal Chem

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Metabolomics and lipidomics are new drivers of the omics era as molecular signatures and selected analytes allow phenotypic characterization and serve as biomarkers, respectively. The growing capabilities of untargeted and targeted workflows, which primarily rely on mass spectrometric platforms, enable extensive charting or identification of bioactive metabolites and lipids. Structural annotation of these compounds is key in order to link specific molecular entities to defined biochemical functions or phenotypes. Tandem mass spectrometry (MS), first and foremost collision-induced dissociation (CID), is the method of choice to unveil structural details of metabolites and lipids. But CID fragment ions are often not sufficient to fully characterize analytes. Therefore, recent years have seen a surge in alternative tandem MS methodologies that aim to offer full structural characterization of metabolites and lipids. In this article, principles, capabilities, drawbacks, and first applications of these “advanced tandem mass spectrometry” strategies will be critically reviewed. This includes tandem MS methods that are based on electrons, photons, and ion/molecule, as well as ion/ion reactions, combining tandem MS with concepts from optical spectroscopy and making use of derivatization strategies. In the final sections of this review, the first applications of these methodologies in combination with liquid chromatography or mass spectrometry imaging are highlighted and future perspectives for research in metabolomics and lipidomics are discussed. Graphical abstract

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“…The laser light from the MALDI process is used to drive the PB reaction and allow for CC double bond localization in GPLs. Other recent reports have demonstrated the use of online oxidation of CC double bonds in DESI imaging mass spectrometry experiments to characterize unsaturated lipid isomers. , Other ion dissociation methods may also ultimately find greater utility in lipid analyses. For example, metastable atom-activated dissociation (MAD) and surface induced dissociation (SID) have enjoyed success in protein and peptide sequencing and have also shown promising results for lipid structural elucidation.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Perspective on Emerging Mass Spectrometry Technologies for Comprehensive Lipid Structural Elucidation

Bonney

Prentice

2021

Anal. Chem.

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Lipids and metabolites are of interest in many clinical and research settings because it is the metabolome that is increasingly recognized as a more dynamic and sensitive molecular measure of phenotype. The enormous diversity of lipid structures and the importance of biological structure–function relationships in a wide variety of applications makes accurate identification a challenging yet crucial area of research in the lipid community. Indeed, subtle differences in the chemical structures of lipids can have important implications in cellular metabolism and many disease pathologies. The speed, sensitivity, and molecular specificity afforded by modern mass spectrometry has led to its widespread adoption in the field of lipidomics on many different instrument platforms and experimental workflows. However, unambiguous and complete structural identification of lipids by mass spectrometry remains challenging. Increasingly sophisticated tandem mass spectrometry (MS/MS) approaches are now being developed and seamlessly integrated into lipidomics workflows to meet this challenge. These approaches generally either (i) alter the type of ion that is interrogated or (ii) alter the dissociation method in order to improve the structural information obtained from the MS/MS experiment. In this Perspective, we highlight recent advances in both ion type alteration and ion dissociation methods for lipid identification by mass spectrometry. This discussion is aimed to engage investigators involved in fundamental ion chemistry and technology developments as well as practitioners of lipidomics and its many applications. The rapid rate of technology development in recent years has accelerated and strengthened the ties between these two research communities. We identify the common characteristics and practical figures of merit of these emerging approaches and discuss ways these may catalyze future directions of lipid structural elucidation research.

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Rapid Imaging of Unsaturated Lipids at an Isomeric Level Achieved by Controllable Oxidation

Cited by 18 publications

References 33 publications

Applications of ambient ionization mass spectrometry in 2021: An annual review

Applications of ambient ionization mass spectrometry in 2021: An annual review

Advanced tandem mass spectrometry in metabolomics and lipidomics—methods and applications

Perspective on Emerging Mass Spectrometry Technologies for Comprehensive Lipid Structural Elucidation

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