Rapid identification of H5 avian influenza virus in chicken throat swab specimens using microfluidic real-time RT-PCR

Zhu, Ling; Zhu, Cancan; Deng, Guoqing; Zhang, Long; Zhao, Shumi; Lin, Jianhan; Li, Li; Jiao, Peirong; Liao, Ming; Liu, Yong

doi:10.1039/c3ay42126k

Cited by 12 publications

(5 citation statements)

References 21 publications

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“…This compact design not only improves the integration of the excitation light source, but also helps to realize the portability of the real-time uorescence detection system. 18,19 The light emitted from the LED can obtain the horizontal excitation light aer the collimation of the plano-convex lens, and split by the dichroic mirror, nally forming a parallel, uniform outgoing light, incident light source channel box to complete the excitation of the detection channel, and its working principle diagram is shown in Fig. 3(a).…”

Section: System Design and Instrumentationmentioning

confidence: 99%

Sophisticated and precise: design and implementation of a real-time optical detection system for ultra-fast PCR

Huang

et al. 2023

RSC Adv.

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Section: System Design and Instrumentationmentioning

confidence: 99%

Sophisticated and precise: design and implementation of a real-time optical detection system for ultra-fast PCR

Huang

et al. 2023

RSC Adv.

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“…The temperature control subsystem, as we reported previously [28,29], was used to maintain temperature conditions for sample lysis and PCR progress, as shown in Figure 2d. It consists of a heat sink wrapped in a fixed box, a Peltier element (Shenzhen Yangming Hongye Technology Co., Ltd., Shenzhen, China), cooling fins, and a cooling fan.…”

Section: Custom-made Poc Devicementioning

confidence: 99%

A Novel Microfluidic Device Integrated with Chitosan-Modified Capillaries for Rapid ZIKV Detection

Zhu

Zhao

et al. 2020

Micromachines

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The outbreak of Zika virus (ZIKV) has posed a great challenge to public health in recent years. To address the urgent need of ZIKV RNA assays, we integrate the microfluidic chip embedded with chitosan-modified silicon dioxide capillaries, smartphone-based detection unit to be a C3-system for the rapid extraction and detection of ZIKV RNA. The C3-system is characterized by: (1) four chitosan-modified silicon dioxide capillaries integrated in the microfluidic chip for target ZIKV RNA enrichment and “in situ PCR” (polymerase chain reaction) amplification; (2) smartphone-based point of care (POC) device consisting of a pneumatic subsystem for controlling the nucleic acid extraction processes in the microfluidic chip, a heating subsystem for sample lysis and PCR amplification, and an optical subsystem for signal acquisition. The entire detection processes including sample lysis, ZIKV RNA enrichment, and reverse-transcription polymerase chain reaction (RT-PCR) is achieved in the microfluidic chip. Moreover, PCR buffers can be directly loaded into the chitosan-modified silicon dioxide capillaries for “in situ PCR”, in which the captured ZIKV RNA is directly used for downstream PCR without any loss. ZIKV RNA extracted by the C3-system can be successfully recovered at very low concentrations of 50 transducing units (TU)/mL from crude human saliva. This means that our method of detecting viremia in patients infected with ZIKV is reliable.

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“…The microfluidic chip was fabricated through traditional protocols reported previously [22]. Briefly, inverse patterns of the desired structures were produced through a machining process on metal moulds.…”

Section: Chip Design and Fabricationmentioning

confidence: 99%

A microfluidic system integrated one-step PCR and high-resolution melting analysis for rapid rice mutant detection

Zhu

et al. 2019

Biotechnology & Biotechnological Equipment

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Rice is a crucial crop to meet global food demands, therefore, it is necessary to develop rapid, efficient and inexpensive methods for screening mutants of important genes in molecular breeding. In this research, a simple microfluidic chip-integrated one-step polymerase chain reaction (PCR) and high-resolution melting (HRM) analysis were designed for the rapid screening of rice mutants. In order to complete the above processes, we also installed a self-developed functional instrument integrated with a temperature control system and a fluorescence detection department. This microfluidic platform was validated by analysing genes related to insect resistance, phenotype and yield of two important varieties of rice, Oryza sativa L. ssp. japonica. and Oryza sativa L. ssp. indica (93-11). The results showed that the platform could detect singlenucleotide mutations between the wild and the mutant rice plants in 1 h and the accuracy was consistent with gene sequencing regarded as the golden standard of the mutants screening. Compared to the traditional method (requiring approximately 3 h), this platform can distinguish mutants from wild types more rapidly. This system is a simple, integrated, disposable and lowcost tool for high-throughput screening of mutants in molecular breeding. More important, this portable instrument can be used as a point-of-care platform for nucleic acid diagnostics in many scientific fields. ARTICLE HISTORY

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Rapid identification of H5 avian influenza virus in chicken throat swab specimens using microfluidic real-time RT-PCR

Cited by 12 publications

References 21 publications

Sophisticated and precise: design and implementation of a real-time optical detection system for ultra-fast PCR

Sophisticated and precise: design and implementation of a real-time optical detection system for ultra-fast PCR

A Novel Microfluidic Device Integrated with Chitosan-Modified Capillaries for Rapid ZIKV Detection

A microfluidic system integrated one-step PCR and high-resolution melting analysis for rapid rice mutant detection

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