Rapid identification of fatty acids and (O-acyl)-ω-hydroxy fatty acids in human meibum by liquid chromatography/high-resolution mass spectrometry

Mori, Naoto; Fukano, Yuya; Arita, Reiko; Shirakawa, Rika; Kawazu, Kouichi; Nakamura, Masatsugu; Amano, Shiro

doi:10.1016/j.chroma.2014.04.082

Cited by 34 publications

(36 citation statements)

References 29 publications

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“…More than a hundred major individual lipid species that belong to a dozen or more lipid classes were found in meibum of humans and many mammals and described in detail (Butovich et al, 2007b) (Butovich et al, 2007a) (Butovich, 2008, 2009a, 2010a; Butovich et al, 2012a; Butovich et al, 2011; Butovich et al, 2012b; Butovich et al, 2009; Chen et al, 2010; Lam et al, 2011) (Saville et al, 2011) (Campbell et al, 2011) (Brown et al, 2013) (Lam et al, 2014c) (Mori et al, 2014). Despite the continuing search for progressively minor new members of the already identified families of meibomian lipids (Chen et al, 2010; Chen et al, 2013, 2015), major components of meibum, and their average abundances, are now known (Table 1).…”

Section: Lipids Of Meibummentioning

confidence: 99%

Meibomian glands, meibum, and meibogenesis

Butovich

2017

Experimental Eye Research

106

118

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Meibum is a lipid-rich secretion that is produced by fully differentiated meibocytes in the holocrine Meibomian glands (MG) of humans and most mammals. The secretion is a part of a defense mechanism that protects the ocular surface from hazardous environmental factors, and from desiccation. Meibomian lipids that have been identified in meibum are very diverse and unique in nature. The lipid composition of meibum is different from virtually any other lipid pool found in the human body. In fact, meibum is quite different from sebum, which is the closest secretion that is produced by anatomically, physiologically, and biochemically related sebaceous glands. However, meibum of mice have been shown to closely resemble that of humans, implying similar biosynthetic mechanisms in MG of both species. By analyzing available genomic, immunohistochemical, and lipidomic data, we have envisioned a unifying network of enzymatic reactions that are responsible for biosynthesis of meibum, which we call meibogenesis. Our current theory is based on an assumption that most of the biosynthetic reactions of meibogenesis are catalyzed by known enzymes. However, the main features that make meibum unique – the ratio of identified classes of lipids, the extreme length of its components, extensive ω-hydroxylation of fatty acids and alcohols, iso- and anteiso-branching of meibomian lipids (e.g. waxes), and the presence of rather unique complex lipids with several ester bonds – make it possible that either the activity of known enzymes is altered in MG, or some unknown enzymes contribute to the processes of meibogenesis, or both. Studies are in progress to elucidate meibogenesis on molecular level.

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Section: Lipids Of Meibummentioning

confidence: 99%

Meibomian glands, meibum, and meibogenesis

Butovich

2017

Experimental Eye Research

106

118

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“…Mori and co-workers succeeded identifying 61 OAH-FA molecular species with 34-56 carbon atoms by using this method. The species with the highest abundance were C18:1/32:1, C18:1/30:1, C16:1/32:1 (25).…”

Section: Lc Separation Of Famentioning

confidence: 99%

“…These species decrease as the severity of dry eye disease increase, so they can be considered as a potential biomarker in meibomian gland dysfunction. It is important to elucidate the relationship between odd number FA and bacterial infections in meibomian gland dysfunction patients, because bacteria can produce an odd number of FAs (25). On the other hand many hydroxyl-derived compounds of EPA and DHA are involved in the regulation of vascular tone, arteriosclerosis and are considered markers of lipid peroxidation (30).…”

Section: Potential Clinical Utility Of Fatty Acids Profile Analysismentioning

confidence: 99%

“…Not only human serum can be analyzed by using a HPLC method, but different lipid species were separated from human meibum samples. In this case lipids were dissolved in a chloroform/ methanol mixture, and the extracts were dried by using a centrifugal concentrator (25).…”

Section: Sample Preparationmentioning

confidence: 99%

“…However, liquid-chromatography (4,17,(23)(24)(25)(26)(27)(28)(29)(30)(31) has been presented by several authors and also, there are few articles which deal with other techniques such as capillary electrophoresis or infrared (IR) and Raman spectroscopy (16,31,32).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Recent developments in fatty acids profile determination in biological samples - a review

Tiuca

Nagy

Oprean

2015

Revista Romana De Medicina De Laborator

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Determination of trans‐fatty acids in food samples based on the precolumn fluorescence derivatization by high performance liquid chromatography

Wang

Guo

Fan

et al. 2022

J of Separation Science

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Trans-fatty acids are unsaturated fatty acids that are considered to have health risks. 1,3,5,7-Tetramethyl-8-butyrethylenediamine-difluoroboradiaza-s-indacene is a highly sensitive fluorescent labeling reagent for carboxylic acids developed by our lab. In this study, using this precolumn fluorescent derivatization reagent, a rapid and accurate high-performance liquid chromatography with fluorescence detection method was developed for the determination of two trans-fatty acids in food samples. Under the optimized derivative conditions, two trans-fatty acids were tagged with the fluorescent labeling reagent in the presence of 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide at 25 • C for 30 min. Then, the baseline separation of trans-and cis-fatty acids and their saturated fatty acid with similar structures was achieved with less interference using a reversed-phased C 18 column with isocratic elution in 14 min. With fluorescence detection at λ ex /λ em = 490 /510 nm, the linear range of the TFAs was 1.0-200 nM with low detection limits in the range of 0.1-0.2 nM (signal-to-noise ratio = 3). In addition, the proposed approach was successfully applied for the detection of trans-fatty acids in food samples, and the recoveries using this method ranged from 96.02 to 109.22% with low relative standard deviations of 1.2-4.3% (n = 6).

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Rapid identification of fatty acids and (O-acyl)-ω-hydroxy fatty acids in human meibum by liquid chromatography/high-resolution mass spectrometry

Cited by 34 publications

References 29 publications

Meibomian glands, meibum, and meibogenesis

Meibomian glands, meibum, and meibogenesis

Recent developments in fatty acids profile determination in biological samples - a review

Determination of trans‐fatty acids in food samples based on the precolumn fluorescence derivatization by high performance liquid chromatography

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