Rapid Identification of Candida Species from Positive Blood Cultures by Use of the FilmArray Blood Culture Identification Panel

Simor, Andrew E.; Porter, Vanessa; Mubareka, Samira; Chouinard, Marc; Katz, Kevin; Vermeiren, Christie; Fattouh, Ramzi; Matukas, Larissa; Tadros, Manal; Mazzulli, Tony; Poutanen, Susan M.

doi:10.1128/jcm.01387-18

Cited by 16 publications

(11 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Three commercially available molecular tools have been applied to rapidly identify Candida spp. directly from positive blood culture bottles (without waiting for the growth of the organisms on the subsequent culture media): the Candida PNA FISH ® assay (OpGen ® ) (5, 6), the BioFire ® FilmArray ® Blood Culture Identification Panel (BioMerieux ® ) (7), and PhenoTest ® Blood Culture kit (Accelerate Diagnostics) (8). One major limitation of each method is the lack of broad coverage for fungal pathogen detection since the former two methods only target five…”

Section: Introductionmentioning

confidence: 99%

Multicenter Evaluation of a PCR-Based Digital Microfluidics and Electrochemical Detection System for the Rapid Identification of 15 Fungal Pathogens Directly from Positive Blood Cultures

et al. 2020

View full text Add to dashboard Cite

Routine identification of fungal pathogens from positive blood cultures by culture-based methods can be time-consuming, delaying treatment with appropriate antifungal agents. The GenMark Dx ePlex investigational use only blood culture identification fungal pathogen panel (BCID-FP) rapidly detects 15 fungal targets simultaneously in blood culture samples positive for fungi by Gram staining. We aimed to determine the performance of the BCID-FP in a multicenter clinical study. Blood culture samples collected at 10 United States sites and tested with BCID-FP at 4 sites were compared to the standard-of-care microbiological and biochemical techniques, fluorescence in situ hybridization using peptide nucleic acid probes (PNA-FISH) and matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS). Discrepant results were analyzed by bi-directional PCR/sequencing of residual blood culture samples. A total of 866 clinical samples, 120 retrospectively and 21 prospectively collected, along with 725 contrived samples were evaluated. Sensitivity and specificity of detection of Candida species (C. albicans, C. auris, C. dubliniensis, C. famata, C. glabrata, C. guilliermondii, C. kefyr, C. krusei, C. lusitaniae, C. parapsilosis, and C. tropicalis) ranged from 97.1 to 100% and 99.8 to 100%, respectively. For the other organism targets, sensitivity and specificity were as follows: 100% each for Cryptococcus neoformans and C. gattii, 98.6% and 100% for Fusarium spp., and 96.2% and 99.9% for Rhodotorula spp., respectively. In 4 of the 141 clinical samples, the BCID-FP panel correctly identified an additional Candida species, undetected by standard-of-care methods. The BCID-FP panel offers a faster turnaround time for identification of fungal pathogens in positive blood cultures that may allow for earlier antifungal interventions and includes C. auris, a highly multidrug-resistant fungus.

show abstract

Section: Introductionmentioning

confidence: 99%

Multicenter Evaluation of a PCR-Based Digital Microfluidics and Electrochemical Detection System for the Rapid Identification of 15 Fungal Pathogens Directly from Positive Blood Cultures

et al. 2020

View full text Add to dashboard Cite

show abstract

“…There are few FDA-cleared molecular-based methods capable of identifying fungal pathogens [ 50 ]. Some of them are able to identify them directly from positive blood culture bottles (e.g., FilmArray, Biofire–Biomerieux; Candida PNA FISH assay, OpGen; T2 Candida -Biosystems and SeptiFast-Roche) [ 51 ] or from other clinical samples [ 52 ]. The major common limitation of these methods is the narrow coverage for fungal pathogens and the low impact in the selection of specific antifungal treatment [ 4 , 51 ].…”

Section: Intrinsic Resistance Detectionmentioning

confidence: 99%

Molecular Markers of Antifungal Resistance: Potential Uses in Routine Practice and Future Perspectives

García‐Effrón

2021

JoF

View full text Add to dashboard Cite

Antifungal susceptibility testing (AST) has come to establish itself as a mandatory routine in clinical practice. At the same time, the mycological diagnosis seems to have headed in the direction of non-culture-based methodologies. The downside of these developments is that the strains that cause these infections are not able to be studied for their sensitivity to antifungals. Therefore, at present, the mycological diagnosis is correctly based on laboratory evidence, but the antifungal treatment is undergoing a growing tendency to revert back to being empirical, as it was in the last century. One of the explored options to circumvent these problems is to couple non-cultured based diagnostics with molecular-based detection of intrinsically resistant organisms and the identification of molecular mechanisms of resistance (secondary resistance). The aim of this work is to review the available molecular tools for antifungal resistance detection, their limitations, and their advantages. A comprehensive description of commercially available and in-house methods is included. In addition, gaps in the development of these molecular technologies are discussed.

show abstract

“…Seven isolates belonged to species not targeted by the test; those were not detected. Ten blood cultures contained more than one pathogen, and all pathogens included in the panel were identified correctly [ 43 ]. Since the test panel includes yeasts as well as Gram-positive and Gram-negative bacteria, it is not necessary to perform a Gram-stain prior to the assay.…”

Section: Blood Culture-dependent Molecular Diagnosticsmentioning

confidence: 99%

Molecular Methods for the Diagnosis of Invasive Candidiasis

Camp

Spettel

Willinger

2020

JoF

View full text Add to dashboard Cite

Invasive infections caused by members of the genus Candida are on the rise. Especially patients in intensive care units, immunocompromised patients, and those recovering from abdominal surgery are at risk for the development of candidemia or deep-seated candidiasis. Rapid initiation of appropriate antifungal therapy can increase survival rates significantly. In the past, most of these infections were caused by C. albicans, a species that typically is very susceptible to antifungals. However, in recent years a shift towards infections caused by non-albicans species displaying various susceptibly patterns has been observed and the prompt diagnosis of the underlying species has become an essential factor determining the therapeutic outcome. The gold standard for diagnosing invasive candidiasis is blood culture, even though its sensitivity is low and the time required for species identification usually exceeds 48 h. To overcome these issues, blood culture can be combined with other methods, and a large number of tests have been developed for this purpose. The aim of this review was to give an overview on strengths and limitations of currently available molecular methods for the diagnosis of invasive candidiasis.

show abstract

Rapid Identification of Candida Species from Positive Blood Cultures by Use of the FilmArray Blood Culture Identification Panel

Abstract: identification of Candida species from positive blood cultures by use of the FilmArray blood culture identification panel.

Cited by 16 publications

References 11 publications

Multicenter Evaluation of a PCR-Based Digital Microfluidics and Electrochemical Detection System for the Rapid Identification of 15 Fungal Pathogens Directly from Positive Blood Cultures

Multicenter Evaluation of a PCR-Based Digital Microfluidics and Electrochemical Detection System for the Rapid Identification of 15 Fungal Pathogens Directly from Positive Blood Cultures

Molecular Markers of Antifungal Resistance: Potential Uses in Routine Practice and Future Perspectives

Molecular Methods for the Diagnosis of Invasive Candidiasis

Contact Info

Product

Resources

About