Rapid ex vivo molecular fingerprinting of biofluids using laser-assisted rapid evaporative ionization mass spectrometry

Abstract

L. (2021). Rapid ex vivo molecular fingerprinting of biofluids using laser-assisted rapid evaporative ionization mass spectrometry. Nature Protocols.

Emerging Applications of Mass Spectrometry‐Based Metabolic Fingerprinting in Clinics

“…Furthermore, REI is an ambient ionization technique, thus can achieve direct real-time analysis of biological fluids with almost no sample pretreatment. [13] After MS data acquisition, multivariate statistical methods are required to interpret the information obtained from MS datasets, [14] including unsupervised clustering and supervised machine learning (ML) model. Unsupervised methods, such as principal component analysis (PCA), [15] cluster samples based on the variation of metabolic fingerprints without the prior knowledge of the sample label.…”

“…Furthermore, REI is an ambient ionization technique, thus can achieve direct real‐time analysis of biological fluids with almost no sample pretreatment. [ 13 ]…”

“…REIMS utilizes real‐time metabolic analysis of the aerosol produced from various biological materials without sample preparation, including biological fluids, cell culture lines, human tissue, and microorganisms, [13a] indicating the capability of real‐time diagnosis and especially intraoperative detection.…”

Point-of-care applicable metabotyping using biofluid-specific electrospun MetaSAMPs directly amenable to ambient LA-REIMS

“…To date, metabolomics of human biofluids is emerging as a promising biofocus as it provides advantages that classical diagnostics do not, following discovery of a suite of clinically relevant biomarkers that are simultaneously affected by disease while contributing to the elucidation of metabolic pathways underlying particular phenotypes of health and disease (i.e., metabotyping) (1)(2)(3). The most urgent application of metabolomics remains the identification of pathologies in children as early-infancy exposures, including nutrition and illness, severely affect adult health and functionality as well (4).…”

“…To date, LA-REIMS is the only ambient ionization mass spectrometry (AIMS) platform that has been successfully used for the automated analysis of a range of crude biofluids, including feces, saliva, urine, etc. ( 1 ). LA-REIMS enables an analysis rate of only few min per sample and demonstrates substantial congruencies with mass spectra obtained by conventional metabolomics ( 16 , 21 , 22 ).…”