Rapid detection of urushiol allergens of Toxicodendron genus using leaf spray mass spectrometry

Tadjimukhamedov, Fatkhulla K.; Huang, Guangming; Ouyang, Zheng; Cooks, R. Graham

doi:10.1039/c2an16077c

Cited by 29 publications

(15 citation statements)

References 21 publications

(21 reference statements)

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“…Draper et al (2002) employed atmospheric pressure ionization (API) LC-MS-MS for urushiols determination [ 14 ]. More recently, urushiols were directly detected and identified in poison ivy leaves, without any sample preparation steps, using leaf spray mass spectrometry [ 15 ]. However, the spatial tissue localization of the different urushiol congeners has not been reported, and could reveal important insights into the compartmentalization of the synthesis of urushiols.…”

Section: Introductionmentioning

confidence: 99%

MALDI-MS Imaging of Urushiols in Poison Ivy Stem

et al. 2017

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Urushiols are the allergenic components of Toxicodendron radicans (poison ivy) as well as other Toxicodendron species. They are alk-(en)-yl catechol derivatives with a 15- or 17-carbon side chain having different degrees of unsaturation. Although several methods have been developed for analysis of urushiols in plant tissues, the in situ localization of the different urushiol congeners has not been reported. Here, we report on the first analysis of urushiols in poison ivy stems by matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI). Our results show that the urushiol congeners with 15-carbon side chains are mainly localized to the resin ducts, while those with 17-carbon side chains are widely distributed in cortex and vascular tissues. The presence of these urushiols in stem extracts of poison ivy seedlings was confirmed by GC-MS. These novel findings provide new insights into the spatial tissue distribution of urushiols that might be biosynthetically or functionally relevant.

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Section: Introductionmentioning

confidence: 99%

MALDI-MS Imaging of Urushiols in Poison Ivy Stem

et al. 2017

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“…Leaf spray ionization methods have been used for performing direct analysis of steviol glycosides from stevia leaves [47], phenolic glycosides [48], rapid identification of molecular changes in tulsi (Ocimum sanctum Linn) upon ageing [49], rapid detection of urushiol allergens of toxicodendron genus [50], polyhydroxylated alkaloids in mulberry [51], and pesticide residues [52]. Leaf spray also be used for in situ chemical analysis of raw herbs [53], and distinction of coffee origin [54], phytochemicals in petals [55], and Chinese and Japanese star anise [56].…”

Section: Leaf Spraymentioning

confidence: 99%

Analysis of Biofluids by Paper Spray MS: Advances and Challenges

2016

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Paper spray mass spectrometry (MS) is part of a cohort of ambient ionization or direct analysis methods that seek to analyze complex samples without prior sample preparation. Extraction and electrospray ionization occur directly from the paper substrate upon which a dried matrix spot is stored. Paper spray MS is capable of detecting drugs directly from dried blood, plasma, and urine spots at the low ng/mL to pg/mL levels without sample preparation. No front end separation is performed, so tandem mass spectrometry (MS/MS) or high resolution MS is required. Here, we discuss paper spray methodology, give a comprehensive literature review of the use of paper spray MS for bioanalysis, discuss technological advancements and variations on this technique, and discuss some of its limitations. _________________________________________________________________________________ AbstractPaper spray mass spectrometry (MS) is part of a cohort of ambient ionization or direct analysis methods that seek to analyze complex samples without prior sample preparation. Extraction and electrospray ionization occur directly from the paper substrate upon which a dried matrix spot is stored. Paper spray MS is capable of detecting drugs directly from dried blood, plasma, and urine spots at the low ng/mL to pg/mL levels without sample preparation. No front end separation is performed, so tandem mass spectrometry (MS/MS) or high resolution MS is required. Here, we discuss paper spray methodology, give a comprehensive literature review of the use of paper spray MS for bioanalysis, discuss technological advancements and variations on this technique, and discuss some of its limitations.

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“…Although leaf spray is not useful for the analysis of the attractive biofluid, applications in agricultural impact could be quite significant. Without sample pretreatment such as grinding, offline extraction, or application to external sampling platforms, a relatively quick analysis of the native leaves on a plant can reveal insight to pesticide residues, glycosides, allergens, and potentially other molecules relevant to consumers for quality control that could impact entire populations, save those that are not able to be easily ionized (Malaj et al, ; Sarkar, Srimany, & Pradeep, ; Tadjimukhamedov et al, ; Zhang et al, ; Snyder et al, ).…”

Section: Direct Analysis Of Molecules In Native Statementioning

confidence: 99%

Emerging trends in paper spray mass spectrometry: Microsampling, storage, direct analysis, and applications

Frey

Damon

Badu‐Tawiah

2019

Mass Spectrometry Reviews

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Recent advancements in the sensitivity of chemical instrumentation have led to increased interest in the use of microsamples for translational and biomedical research. Paper substrates are by far the most widely used media for biofluid collection, and mass spectrometry is the preferred method of analysis of the resultant dried blood spot (DBS) samples. Although there have been a variety of review papers published on DBS, there has been no attempt to unify the century old DBS methodology with modern applications utilizing modified paper and paper‐based microfluidics for sampling, storage, processing, and analysis. This critical review will discuss how mass spectrometry has expanded the utility of paper substrates from sample collection and storage, to direct complex mixture analysis to on‐surface reaction monitoring.

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Rapid detection of urushiol allergens of Toxicodendron genus using leaf spray mass spectrometry

Cited by 29 publications

References 21 publications

MALDI-MS Imaging of Urushiols in Poison Ivy Stem

MALDI-MS Imaging of Urushiols in Poison Ivy Stem

Analysis of Biofluids by Paper Spray MS: Advances and Challenges

Emerging trends in paper spray mass spectrometry: Microsampling, storage, direct analysis, and applications

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