Rapid Detection of the Pathogenicity of Phytopathogenic Pseudomonads

Klement, Z.

doi:10.1038/199299b0

Cited by 308 publications

(145 citation statements)

References 3 publications

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“…Isolates were identified on the basis of tests for cytochrome oxidase (Kovacs, 1956), arginine dihydrolase (Thornley, 1960), gelatin liquefaction (Dye, 1968), tobacco HR (Klement, 1963), nitrate reduction, growth at 4 and 41 o C, levan production, (Shaad et al, 2001) and the ability to growth on carbon sources such as L-arabinose, Dgalactose, trehalose, meso-inositol, sorbitol, and L-tartrate in the basal medium of Ayers et al (1919).…”

Section: Methodsmentioning

confidence: 99%

Antagonistic Potential of Fluorescent Pseudomonads and Control of Crown and Root Rot of Cucumber Caused by Phythophtora drechsleri

Shirzad¹,

Fallahzadeh-Mamaghani²,

Pazhouhandeh³

2012

The Plant Pathology Journal

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Section: Methodsmentioning

confidence: 99%

Antagonistic Potential of Fluorescent Pseudomonads and Control of Crown and Root Rot of Cucumber Caused by Phythophtora drechsleri

Shirzad¹,

Fallahzadeh-Mamaghani²,

Pazhouhandeh³

2012

The Plant Pathology Journal

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“…morsprunorum is found only on plants of the genus Prunus. I n common with many other phytopathogenic bacteria, however, both organisms when inoculated under suitable conditions will grow and induce symptoms in plants having little botanical affinity with their natural hosts (Erikson, 1945; Dye, 1958; Logan, 1960; Klement & Lovrekovich, 1961, 1962 Klement, 1963; Klement, Fa.rkas & Lovrekovich, 1964). The following investigation compared the growth of P. phaseolicola and P. morsprunorum in natural hosts of these organisms and i t was undertaken to provide preliminary information on the factors determining host specificity in the field.…”

Section: Introductionmentioning

confidence: 99%

The Growth of Pseudomonas phaseolicola and Related Plant Pathogens in vivo

Ercolani¹,

Crosse²

1966

Journal of General Microbiology

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SUMMARYI n the leaves of their natural (homologous) hosts, the bean and cherry respectively, Pseudomonas phaseolicola and P. morsprunorum increased logarithmically for at least 4 days after inoculation. Transition into the stationary phase was gradual and accompanied by water congestion of the infected tissues, followed by typical field symptoms. Increases in the inoculum dose had relatively little or no effect on the generation time but growth ceased earlier at the higher doses. I n the reciprocal (heterologous) combinations, logarithmic growth was abruptly terminated after 2-3 days, due apparently to a specific defensive reaction in the host. This coincided at the higher inoculum doses with the appearance of dry necrotic symptoms in the leaves. No macroscopic symptoms were observed with the lower doses, and with the lowest dose in bean there was an acceleration of leaf maturation in the presence of heterologous organisms. Generation times were lower in heterologous combinations but increased markedly with the inoculum dosc. The growth of the pear strain of P. syringae in bean and cherry leaves showed typical heterologous characteristics. The final yields of bacteria per unit inoculum were invariably higher in homologous combinations, but they decreased with increasing dose, whereas heterologous yields increased. The differences in net growth response were therefore greatest a t the lowest doses. This suggested that host specificity in the field was associated with factors controlling growth of the organisms in vivo from small initial inocula.

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“…Suspect colonies are purified and identified according their morphology on agar plates and further tested for their ability to elicit a Hypersensitivity Reaction (HR) on bean pods (Klement, 1963) or on tomato leaves cv. 'Roma' (Lelliott & Stead, 1987), or else by IFAS and/or protein profiling.…”

Section: Immunofluorescence (If)mentioning

confidence: 99%