Rapid detection of specific gene mutations associated with isoniazid or rifampicin resistance in Mycobacterium tuberculosis clinical isolates using non-fluorescent low-density DNA microarrays

Aragón, Lina Marcela; Navarro, Ferrán; Heiser, Volker; Garrigó, Montserrat; Español, Montserrat; Coll, Pere

doi:10.1093/jac/dkl058

Cited by 56 publications

(28 citation statements)

References 34 publications

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“…Aragon et al developed a fast low cost and low density DNA microarray (LCD array) for the detection of mutations that confer isoniazid or rifampicin resistance in MTB isolates. They observed that LCD was 100% concordant with the sequencing data for H resistance and 93.8% concordant for R resistance [42]. A sensitive and specific microarray was designed by Yao et al to detect mutations in the rifampin resistance determining region of rpoB and loci in katG and inhA associated with H resistance.…”

Section: Therapy and Susceptibility Testingmentioning

confidence: 99%

Cost-effectiveness in the diagnosis of tuberculosis: choices in developing countries

Molicotti

Bua

Zanetti

2014

J Infect Dev Ctries

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Tuberculosis remains one of the major causes of global death from a single infectious agent. This situation is worsened by the HIV/AIDS pandemic because one-third of HIV/AIDS patients are co-infected with Mycobacterium tuberculosis. Failure to control the spread of tuberculosis is largely due to our inability to detect and treat all infectious cases of pulmonary tuberculosis in a timely manner, allowing continued M. tuberculosis transmission within communities. Diagnosis of tuberculosis can be made using indirect and direct methods. The indirect tests, such as interferon-gamma release assays, provide a new diagnostic method for M. tuberculosis infection, but do not discriminate between infection and active disease. The most common direct method for diagnosing TB worldwide is sputum smear microscopy (developed more than 100 years ago), where bacteria are observed in sputum samples examined under a microscope. In countries with more developed laboratory capacities, cases of tuberculosis may also be diagnosed using culture methods (the current gold standard) or, increasingly, using rapid molecular tests. In this review, we discuss the traditional methods for the diagnosis of tuberculosis. We also discuss other inexpensive assays that can be used to detect the presence of M. tuberculosis.

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Section: Therapy and Susceptibility Testingmentioning

confidence: 99%

Cost-effectiveness in the diagnosis of tuberculosis: choices in developing countries

Molicotti

Bua

Zanetti

2014

J Infect Dev Ctries

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“…A low-cost and -density DNA microarray was designed to detect mutations that confer isoniazid and rifampin resistance in M. tuberculosis isolates. The low-cost and -density array protocol takes 45 min after PCR amplification, with only minimal laboratory equipment required (Aragon et al, 2006). Antonova and colleagues developed a method for the detection and identification of mutations in the M. tuberculosis genome determining resistance to fluoroquinolones by hybridization on biological microchips (Antonova et al, 2008).…”

Section: Gene Expression Profiles Of Drugs Resistance Inhibitors Anmentioning

confidence: 99%

DNA microarrays and their applications in medical microbiology

Nsofor¹

2014

Biotechnol. Mol. Biol. Rev.

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Rapid diagnosis and treatment of disease is often based on the identification and characterization of causative agents derived from phenotypic characteristics. This can be laborious and time consuming, often requiring many skilled personnel and a large amount of lab space. However, the introduction of nucleic acid amplification techniques into molecular biology has transformed the laboratory detection of pathogens. The progression of the molecular diagnostic revolution currently relies on the ability to efficiently and accurately offer multiplex detection and characterization for a variety of infectious disease pathogens. DNA microarray analysis has the capability to offer robust multiplex detection. Multiple microarray platforms exist, including printed double-stranded DNA and oligonucleotide arrays, in situ-synthesized arrays, high-density bead arrays, electronic microarrays, and suspension bead arrays. The aim of this paper was to review DNA microarray technology, highlighting two major types: the oligonucleotide-based array and the PCR product-based array. Although, the use of microarrays to generate gene expression data has become routine, applications pertinent to microbiology continue to rapidly expand. This review highlights uses of microarray technology that impact diagnostic microbiology, including the detection and identification of pathogens, determination of antimicrobial resistance, epidemiological strain typing, and determination of virulence factors.

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“…For example, it has been used to distinguish different strains of Pseudomonas [6] or Mycobacterium tuberculosis [7]. The microarray has even been used to characterize mutations in the rpoB and katG genes that confer rifampin and isoniazid resistance in M. tuberculosis [8]. Microarrays are also being incorporated in some clinical laboratories for the rapid detection and classification of methicillin-resistant Staphylococcus aureus, determination of antimicrobial drug resistance in several pathogens such as Enterococcus and Mycobacterium tuberculosis, as well in diagnosis of sepsis [8][9][10].…”

Section: Bacteria Detectionmentioning

confidence: 99%

“…The microarray has even been used to characterize mutations in the rpoB and katG genes that confer rifampin and isoniazid resistance in M. tuberculosis [8]. Microarrays are also being incorporated in some clinical laboratories for the rapid detection and classification of methicillin-resistant Staphylococcus aureus, determination of antimicrobial drug resistance in several pathogens such as Enterococcus and Mycobacterium tuberculosis, as well in diagnosis of sepsis [8][9][10]. Besides detection and identification of pathogens, microarrays are a good option for characterizing genetic differences between isolates of the same species to the strain level.…”

Section: Bacteria Detectionmentioning

confidence: 99%

Infectious diseases detection by microarray: An overview of clinical relevant infections

Herrera-Rodríguez¹,

Elizondo-Quiroga²,

Álvarez-Maya³

2013

JBiSE

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Microarray technology is a powerful tool to investigate whole genome expression profiles to study the crosstalk between pathogens and associated hosts that cause illness. Microarrays have been used in several comparative genome hybridization studies of pathogens. In addition to detection and identification of pathogens, microarrays are ideal for characterizing genetic differences between bacteria isolates of the same species to the strain level. Furthermore, the use of microarrays has been gaining importance in the detection of viral agents. Here we explore the use of microarrays for simultaneous detection of viruses and modifications made over these techniques. Microarray technology has also been incorporated to compensate for time-consuming sequencing. The procedure of fungi identification based on sequences and studies reported the use of microarrays to identify pathogenic yeasts and molds by targeting the internal transcribed spacer regions in fungal rRNA genes. The remarkable advancement in genomics over the last decade has made it possible for microarray technology to evolve towards being the best option for clinical diagnostics because they have several advantages.

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Rapid detection of specific gene mutations associated with isoniazid or rifampicin resistance in Mycobacterium tuberculosis clinical isolates using non-fluorescent low-density DNA microarrays

Cited by 56 publications

References 34 publications

Cost-effectiveness in the diagnosis of tuberculosis: choices in developing countries

Cost-effectiveness in the diagnosis of tuberculosis: choices in developing countries

DNA microarrays and their applications in medical microbiology

Infectious diseases detection by microarray: An overview of clinical relevant infections

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