Rapid detection of Salmonella from hydrodynamic pressure-treated poultry using molecular beacon real-time PCR

Patel, Jitendra; Bhagwat, Arvind A.; Sanglay, Gabriel C.; Solomon, M.B.

doi:10.1016/j.fm.2005.01.011

Cited by 72 publications

(46 citation statements)

References 41 publications

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“…Incorporation of a 6 h non-selective pre-enrichment step further increased the detection limit to 26 cfu/mℓ. Similar results have been reported previously by other authors (Bej et al, 1994;Ellingson et al, 2004;Fratamico, 2003;Guo et al, 2000;Myint et al, 2006;Oliveira et al, 2002;Patel et al, 2006;Pan and Liu, 2002;Soumet et al, 1994) who observed that at least 10 3 to 10 5 cells/mℓ must be present to give positive results by PCR without a pre-enrichment step and that 1 to 10 cells/mℓ could be detected after a pre-enrichment step.…”

Section: Discussionsupporting

confidence: 87%

“…The assay combines simplicity with specificity and sensitivity in detecting organisms. PCR assay has been used successfully to diagnose the presence of bacterial pathogens in aquatic environments, food products and clinical samples (Chen and Griffiths, 2001;Ellingson et al, 2004;Guo et al, 2000;Myint et al, 2006;Patel et al, 2006;Sandery et al, 1996;Schrank et al, 2001;Soumet et al, 1999(a);Soumet et al, 1999(b) detection. Pre-enrichment of samples is thus necessary to lower the detection limit and dilute any inhibitory substances present in the samples.…”

Section: Introductionmentioning

confidence: 99%

“…Pre-enrichment of samples is thus necessary to lower the detection limit and dilute any inhibitory substances present in the samples. Most PCR assays carried out currently include a minimum of 6 to 8 h pre-cultivation step (Ellingson et al, 2004;Fratamico, 2003;Guo et al, 2000;Myint et al, 2006;Oliveira et al, 2002;Oliveira et al, 2003;Patel et al, 2006;Wang and Yeh, 2002) that can increase the sensitivity to one digit number of CFU, which is much better than the immunoassays that preenrich the samples for 24 to 30 h but still obtain minimum detection limits of 10 3 CFU (Soumet et al, 1999(a);Liu et al, 2001;Chen and Durst, 2006). Rahn et al (1992) reported the invA primer set that was able to discriminate between Salmonella and non-Salmonella species.…”

Section: Introductionmentioning

confidence: 99%

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Optimisation of the PCR-invA primers for the detection of Salmonella in drinking and surface waters following a pre-cultivation step

Moganedi¹,

Goyvaerts²,

Venter³

et al. 2009

WSA

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A polymerase chain reaction (PCR)-based method for the detection of Salmonella species in water samples was optimised and evaluated for speed, specificity and sensitivity. Optimisation of Mg 2+ and primer concentrations and cycling parameters increased the sensitivity and limit of detection of PCR to 2.6 x 10 4 cfu/mℓ. A 6h non-selective pre-enrichment step further increased the limit of detection to 26 cfu/mℓ. Out of 14 different Salmonella strains tested, only two, Salmonella arizonae and Salmonella pullorum, did not give positive amplification results with primers homologous to a conserved region of the invA gene. When environmental and drinking waters were assessed, a non-selective pre-enrichment step was included to increase the detection efficiency of PCR. The PCR method demonstrated specificity in the presence of other competing micro-organisms as confirmed by the conventional culture method. No false positives or negatives were observed when household and environmental water samples were tested by invA-PCR analysis parallel to the culture method.

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Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Optimisation of the PCR-invA primers for the detection of Salmonella in drinking and surface waters following a pre-cultivation step

Moganedi¹,

Goyvaerts²,

Venter³

et al. 2009

WSA

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show abstract

“…Conventional culture methods used for the detection of Salmonella include nonselective pre-enrichment, followed by enrichment and plating on selective and differential agars. Suspected colonies are confirmed biochemically and serologically, possibly taking up to 7 days to complete the entire procedure (PatEl et al, 2006).…”

mentioning

confidence: 99%

Rapid detection ofSalmonellain food by redox-potential measurement based method combined with real-time PCR

Erdősi¹,

Szakmár²,

Reichart³

et al. 2014

Acta Alimentaria

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The classical ISO (2002) standard as reference method and the combination of redox potential measurement with real-time PCR technique were applied to detect Salmonella in milk, egg, broiler meat, and artificially contaminated egg samples. Food samples of 25 g were homogenized in 225 ml of RVS broth to prepare the basic suspension of the comparative tests. In the combined method the redox potential measurement technique serves as the selective enrichment system of the real-time PCR equipment. The reliable screening of Salmonella-free, negative samples by the redox potential measurement technique needed only 24 h. These negative samples determined by the PCR and the classical standard method in all cases proved to be negative as well. In case of positive redox result the Salmonella from the enriched suspension of the redox test-cell was identified by real-time PCR in 3 hours, instead of the conventional biochemical identification. Comparing our protocol to the ISO (2002) standard method, the total detection time of Salmonella presence/absence was less than 24 h contrary to the 114 h of the conventional method.

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“…Now, a number of rapid methods for detection of Salmonella have been developed including immuno-assays, nucleic acid hybridization, and polymerase chain reaction (PCR) techniques 3 . PCR assays for the rapid, sensitive, and specific detection of Salmonella.…”

mentioning

confidence: 99%

Broyler Piliçlerin Gastrointestinal Sistemindeki Salmonella spp’nin Polimeraz Zincir Reaksiyonu ile Belirlenmesi

Mirhosseini¹,

Seidavi²,

Saifeddine³

et al. 2009

Kafkas Univ Vet Fak Derg

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Rapid detection of Salmonella from hydrodynamic pressure-treated poultry using molecular beacon real-time PCR

Cited by 72 publications

References 41 publications

Optimisation of the PCR-invA primers for the detection of Salmonella in drinking and surface waters following a pre-cultivation step

Optimisation of the PCR-invA primers for the detection of Salmonella in drinking and surface waters following a pre-cultivation step

Rapid detection ofSalmonellain food by redox-potential measurement based method combined with real-time PCR

Broyler Piliçlerin Gastrointestinal Sistemindeki Salmonella spp’nin Polimeraz Zincir Reaksiyonu ile Belirlenmesi

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