Rapid detection of <i>Salmonella</i> using an aptamer-functionalized PDA liposome sensor with naked-eye colorimetric sensing

Lee, Goeun; Kim, Byeongsung; Jang, Inseung; Il Kim, Moon; Shin, Seunghan; Kwon, Kiok

doi:10.1039/d3ma00840a

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2024

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Cited by 3 publications

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Machine learning-enabled colorimetric sensors for foodborne pathogen detection

Holliday,

Zhang

2024

Advances in Food and Nutrition Research

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Machine learning-enabled colorimetric sensors for foodborne pathogen detection

Holliday,

Zhang

2024

Advances in Food and Nutrition Research

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Ultra-sensitive and rapid detection of Salmonella enterica and Staphylococcus aureus to single-cell level by aptamer-functionalized carbon nanotube field-effect transistor biosensors

Feng,

Li,

et al. 2024

Biosensors and Bioelectronics

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Antibody-Functionalized Gold Nanoparticles as a Highly Sensitive Two-Step Colorimetric Biosensor for Detecting Salmonella Typhimurium in Food

Anzevino,

Marra,

Fulgione

et al. 2024

ACS Appl. Nano Mater.

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The standard laboratory methods used to detect Salmonella are time-consuming, leading to unavoidable delays, which can be costly (e.g., food checks at customs) or even dangerous when quick alarms are needed (e.g., for potential outbreaks). To address this problem, we propose a rapid two-step colorimetric immunoassay. In the first step, gold nanoparticles interact with the sample, while in the second step, an optimized amount of saline solution is added to induce cluster formation among the unbound nanoparticles, providing a color change that inversely correlates with the bacteria concentration. The nanoparticles were functionalized using a technique based on UV activation of the antibodies, resulting in oriented antibodies with antigen-binding regions in the proximity of the surface. While a color change can be observed with the naked eye at relatively higher bacterial concentrations, a more quantitative approach based on UV−visible spectrum measurements allowed us to achieve a limit of detection of 1 cfu/mL in chicken samples. The test can be completed in 15 min and performed on-site, paving the way for widespread applications of our biosensor in the field of food safety.

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Rapid detection of Salmonella using an aptamer-functionalized PDA liposome sensor with naked-eye colorimetric sensing

Abstract: The increasing occurrence of Salmonella pathogens responsible for foodborne diseases is a mounting global apprehension within the sphere of public health and consumer welfare. Detecting Salmonella holds pivotal importance in...

Cited by 3 publications

References 48 publications

Machine learning-enabled colorimetric sensors for foodborne pathogen detection

Machine learning-enabled colorimetric sensors for foodborne pathogen detection

Ultra-sensitive and rapid detection of Salmonella enterica and Staphylococcus aureus to single-cell level by aptamer-functionalized carbon nanotube field-effect transistor biosensors

Antibody-Functionalized Gold Nanoparticles as a Highly Sensitive Two-Step Colorimetric Biosensor for Detecting Salmonella Typhimurium in Food

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