Rapid Detection of Acanthamoeba Cysts in Corneal Scrapings by Lactophenol Cotton Blue Staining

Comparison of Topical Itraconazole 1% With Topical Natamycin 5% for the Treatment of Filamentous Fungal Keratitis

“…13,14 Briefly, material was scraped from the base and edges of each corneal ulcer by a sterile, blunt cataract knife. Some material was used to prepare lactophenol cotton blue-stained wet preparations (for detection of fungi and Acanthamoeba cysts) 14,15 and Gram-stained smears for direct microscopic examination. 13,14 Corneal material was also inoculated in rows of C streaks onto plates of 5% sheep blood agar (incubated at 25°and 37°C for isolation of fungi and bacteria, respectively), cystine tryptone agar (incubated at 37°C for bacterial isolation), and Sabouraud glucose neopeptone agar (incubated at 25°and 37°C for isolation of fungi) and into bottles of thioglycollate medium (for isolation of anaerobic organisms).…”

Comparison of Topical Itraconazole 1% With Topical Natamycin 5% for the Treatment of Filamentous Fungal Keratitis

“…13,14 Briefly, material was scraped from the base and edges of each corneal ulcer by a sterile, blunt cataract knife. Some material was used to prepare lactophenol cotton blue-stained wet preparations (for detection of fungi and Acanthamoeba cysts) 14,15 and Gram-stained smears for direct microscopic examination. 13,14 Corneal material was also inoculated in rows of C streaks onto plates of 5% sheep blood agar (incubated at 25°and 37°C for isolation of fungi and bacteria, respectively), cystine tryptone agar (incubated at 37°C for bacterial isolation), and Sabouraud glucose neopeptone agar (incubated at 25°and 37°C for isolation of fungi) and into bottles of thioglycollate medium (for isolation of anaerobic organisms).…”

A quantitative method to evaluate neutralizer toxicity against Acanthamoeba castellanii

“…Also, stains such as lactophenol cotton blue have been used for rapid detection of cysts in tissue samples. However, trophozoites did not stain as well as cysts (29). It is evident that there is a need for standardized methods for the assessment of contact lens disinfection systems against Acanthamoeba organisms.…”

Twenty Years of Acanthamoeba Diagnostics in Austria

“…Lactophenol cotton blue stain (LPCB) was performed according to the protocol of Thomas and Kuriakose ( 1990 ). Briefly, pellet suspensions were mixed with the aliquot volume of LPCB stain (Sigma-Aldrich, Vienna, Austria) and stained slides were investigated by light microscopy on a Nikon TMS.…”

Comparison of Topical Itraconazole 1% With Topical Natamycin 5% for the Treatment of Filamentous Fungal Keratitis

“…13,14 Briefly, material was scraped from the base and edges of each corneal ulcer by a sterile, blunt cataract knife. Some material was used to prepare lactophenol cotton blue-stained wet preparations (for detection of fungi and Acanthamoeba cysts) 14,15 and Gram-stained smears for direct microscopic examination. 13,14 Corneal material was also inoculated in rows of C streaks onto plates of 5% sheep blood agar (incubated at 25°and 37°C for isolation of fungi and bacteria, respectively), cystine tryptone agar (incubated at 37°C for bacterial isolation), and Sabouraud glucose neopeptone agar (incubated at 25°and 37°C for isolation of fungi) and into bottles of thioglycollate medium (for isolation of anaerobic organisms).…”

A quantitative method to evaluate neutralizer toxicity against Acanthamoeba castellanii

“…Also, stains such as lactophenol cotton blue have been used for rapid detection of cysts in tissue samples. However, trophozoites did not stain as well as cysts (29). It is evident that there is a need for standardized methods for the assessment of contact lens disinfection systems against Acanthamoeba organisms.…”

Twenty Years of Acanthamoeba Diagnostics in Austria

“…Lactophenol cotton blue stain (LPCB) was performed according to the protocol of Thomas and Kuriakose ( 1990 ). Briefly, pellet suspensions were mixed with the aliquot volume of LPCB stain (Sigma-Aldrich, Vienna, Austria) and stained slides were investigated by light microscopy on a Nikon TMS.…”

Comparison of Topical Itraconazole 1% With Topical Natamycin 5% for the Treatment of Filamentous Fungal Keratitis

“…13,14 Briefly, material was scraped from the base and edges of each corneal ulcer by a sterile, blunt cataract knife. Some material was used to prepare lactophenol cotton blue-stained wet preparations (for detection of fungi and Acanthamoeba cysts) 14,15 and Gram-stained smears for direct microscopic examination. 13,14 Corneal material was also inoculated in rows of C streaks onto plates of 5% sheep blood agar (incubated at 25°and 37°C for isolation of fungi and bacteria, respectively), cystine tryptone agar (incubated at 37°C for bacterial isolation), and Sabouraud glucose neopeptone agar (incubated at 25°and 37°C for isolation of fungi) and into bottles of thioglycollate medium (for isolation of anaerobic organisms).…”

A quantitative method to evaluate neutralizer toxicity against Acanthamoeba castellanii

“…Also, stains such as lactophenol cotton blue have been used for rapid detection of cysts in tissue samples. However, trophozoites did not stain as well as cysts (29). It is evident that there is a need for standardized methods for the assessment of contact lens disinfection systems against Acanthamoeba organisms.…”

Twenty Years of Acanthamoeba Diagnostics in Austria

“…Lactophenol cotton blue stain (LPCB) was performed according to the protocol of Thomas and Kuriakose ( 1990 ). Briefly, pellet suspensions were mixed with the aliquot volume of LPCB stain (Sigma-Aldrich, Vienna, Austria) and stained slides were investigated by light microscopy on a Nikon TMS.…”