Rapid and visual detection of Mycoplasma synoviae by recombinase-aided amplification assay combined with a lateral flow dipstick

Xia, Wenlong; Chen, Ke; Liu, Wensong; Yin, Yulong; Ye, Qian; Ban, Y.; Pu, Yiwen; Zhan, Xingmin; Bian, Hongchun; Yu, Shupei; Han, Kai; Yang, Ling; Wang, Huanli; Fan, Zhongjun

doi:10.1016/j.psj.2022.101860

Cited by 6 publications

(3 citation statements)

References 25 publications

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“…Of the ten paper-based RAA articles incorporated in this review, all reported paper-based detection but did not show paper-based extraction or amplification. Nine articles reported colorimetric detection using tagged probes/primers and AuNPs [ 58 , 59 , 60 , 61 , 62 , 63 , 64 ]. For example, Xu et al used LFIA to detect Amphidinium carterae by implementing a species-specific primer tagged with biotin and a probe labeled with FAM [ 62 ].…”

Section: Raa and Mira With Paper Microfluidicsmentioning

confidence: 99%

Recent Uses of Paper Microfluidics in Isothermal Nucleic Acid Amplification Tests

Reynolds,

Loeffler,

Leigh

et al. 2023

Biosensors

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Isothermal nucleic acid amplification tests have recently gained popularity over polymerase chain reaction (PCR), as they only require a constant temperature and significantly simplify nucleic acid amplification. Recently, numerous attempts have been made to incorporate paper microfluidics into these isothermal amplification tests. Paper microfluidics (including lateral flow strips) have been used to extract nucleic acids, amplify the target gene, and detect amplified products, all toward automating the process. We investigated the literature from 2020 to the present, i.e., since the onset of the COVID-19 pandemic, during which a significant surge in isothermal amplification tests has been observed. Paper microfluidic detection has been used extensively for recombinase polymerase amplification (RPA) and its related methods, along with loop-mediated isothermal amplification (LAMP) and rolling circle amplification (RCA). Detection was conducted primarily with colorimetric and fluorometric methods, although a few publications demonstrated flow distance- and surface-enhanced Raman spectroscopic (SERS)-based detection. A good number of publications could be found that demonstrated both amplification and detection on paper microfluidic platforms. A small number of publications could be found that showed extraction or all three procedures (i.e., fully integrated systems) on paper microfluidic platforms, necessitating the need for future work.

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Section: Raa and Mira With Paper Microfluidicsmentioning

confidence: 99%

Recent Uses of Paper Microfluidics in Isothermal Nucleic Acid Amplification Tests

Reynolds,

Loeffler,

Leigh

et al. 2023

Biosensors

View full text Add to dashboard Cite

show abstract

“…These methods are not convenient to use in limited feeding conditions because of the need for cumbersome precision thermal cycler equipment. Additionally, isothermal amplification technology has rapidly developed in recent years, including recombinase polymerase amplification ( RPA ) and loop-mediated isothermal amplification ( LAMP ) ( Kursa et al, 2015 ; Xia et al, 2022 ). The methods can be completed under constant temperature; however, the specificity is poor, and false-negatives or false-positives are easily generated.…”

Section: Introductionmentioning

confidence: 99%

Rapid and sensitive detection of Mycoplasma synoviae using RPA combined with Pyrococcus furiosus Argonaute

Zhao,

Zhang,

et al. 2024

Poultry Science

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“…When the control line and the test line simultaneously color, a positive result is indicated; a result than can be detected by the human eye. Indeed, LFD is a convenient assay for immediate visualization after the rapid amplification of RPA (Xia et al, 2022). Compared with traditional gel electrophoresis detection, LFDs main advantages are that a result can be observed within 1-2 minutes, without the need for precision instruments such as imagers .…”

Section: Introductionmentioning

confidence: 99%

Establishment of methods for rapid detection of Prymnesium parvum by recombinase polymerase amplification combined with a lateral flow dipstick

Luo

Huang²,

Jiang³

2022

Front. Mar. Sci.

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Prymnesium parvum is a toxic algal bloom (HAB)-forming species. The toxicity of this alga is a result of a collection of compounds known as prymnesins. Prymnesins exert harmful effects upon fish, shellfish, and mollusks, causing huge economic losses. In the present study, a new method was developed for the detection of P. parvum. The novel method utilizes isothermal amplification, known as recombinase polymerase amplification (RPA), in combination with lateral-flow dipstick (LFD). Herein, a set of primers and probes were designed for internal transcribed spacer (ITS) sequences, and a specific and sensitive RPA-LFD rapid detection method was established for P. parvum. Meanwhile, we verified its feasibility for the detection of environmental samples. It was demonstrated that the optimal amplification temperature and time for RPA were 39°C and 15 min. RPA/RPA-LFD was experimentally verified to be specific, demonstrating no cross-reaction with distinct control microalgae, and furthermore, the total time required for the RPA-LFD experiment was 20 min. Meanwhile, the detection limit for the genomic DNA of P. parvum was 1.5×10-1 pg/μL, and the detection limit for plasmids was 2.35 pg/μL. In addition, the results herein revealed that the RPA-LFD assay was 100 times more sensitive than PCR for detection of P. parvum. In conclusion, we developed an RPA-LFD that does not require precision instruments, and can be utilized for rapid on-site detection of P. parvum. In the future, the RPA-LFD can be considered for practical application for environmental detection of the toxic algal species.

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Rapid and visual detection of Mycoplasma synoviae by recombinase-aided amplification assay combined with a lateral flow dipstick

Cited by 6 publications

References 25 publications

Recent Uses of Paper Microfluidics in Isothermal Nucleic Acid Amplification Tests

Recent Uses of Paper Microfluidics in Isothermal Nucleic Acid Amplification Tests

Rapid and sensitive detection of Mycoplasma synoviae using RPA combined with Pyrococcus furiosus Argonaute

Establishment of methods for rapid detection of Prymnesium parvum by recombinase polymerase amplification combined with a lateral flow dipstick

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