Rapid and sensitive point-of-care detection of Orthopoxviruses by ABICAP immunofiltration

Stern, Daniel; Olson, Victoria A.; Smith, Scott K.; Pietraszczyk, Marko; Miller, Lilija; Miethe, Peter; Dorner, Brigitte G.; Nitsche, Andreas

doi:10.1186/s12985-016-0665-5

Cited by 32 publications

(33 citation statements)

References 21 publications

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“…It is also very likely in our case that the assay time could be reduced if needed. In order to facilitate usage in remote areas of the world, it would be advantageous to apply the method presented by Stern et al [51] to dry the antibody-coated columns for storage at ambient temperature.…”

Section: Discussionmentioning

confidence: 99%

Sensitive and rapid detection of cholera toxin subunit B using magnetic frequency mixing detection

et al. 2019

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Cholera is a life-threatening disease caused by the cholera toxin (CT) as produced by some Vibrio cholerae serogroups. In this research we present a method which directly detects the toxin’s B subunit (CTB) in drinking water. For this purpose we performed a magnetic sandwich immunoassay inside a 3D immunofiltration column. We used two different commercially available antibodies to capture CTB and for binding to superparamagnetic beads. ELISA experiments were performed to select the antibody combination. The beads act as labels for the magnetic frequency mixing detection technique. We show that the limit of detection depends on the type of magnetic beads. A nonlinear Hill curve was fitted to the calibration measurements by means of a custom-written python software. We achieved a sensitive and rapid detection of CTB within a broad concentration range from 0.2 ng/ml to more than 700 ng/ml.

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Section: Discussionmentioning

confidence: 99%

Sensitive and rapid detection of cholera toxin subunit B using magnetic frequency mixing detection

et al. 2019

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“…The mass transfer limitations are especially important when detecting large size particles, such as whole viruses, which have low diffusion coefficients. In order to minimize the analyte-surface distance and thus to avoid a mass transfer constraint, viruses are captured not on a planar sensing surface but on porous filters during immunofiltration or immunochromatographic assays, or on nanoparticles. , Ultrahigh sensitivity for such assays was reported, reaching ∼100 viruses per mL . However, we see significant advantages of planar microarrays for virus detection: viruses can be multiplexed with other biomarkers in a technically simple, cheap, but highly sensitive assay, as we demonstrate in the present paper.…”

mentioning

confidence: 62%

“…5,6 The mass transfer limitations are especially important when detecting large size particles, such as whole viruses, which have low diffusion coefficients. In order to minimize the analyte-surface distance and thus to avoid a mass transfer constraint, viruses are captured not on a planar sensing surface but on porous filters during immunofiltration 7 or immunochromatographic 8 assays, or on nanoparticles. 9,10 Ultrahigh sensitivity for such assays was reported, reaching ∼100 viruses per mL.…”

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confidence: 99%

Improving Immunoassay Performance with Cleavable Blocking of Microarrays

2020

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Among the key issues that are commonly associated with the development of microarray-based assays are nonspecific binding and diffusion constraints. Here we present a novel strategy addressing both of these challenges simultaneously. The essence of the method consists in blocking the microarray surface with a blocking agent containing a perfluoroalkyl chain and a disulfide linker. The resulting surface is hydrophobic, and no immiscible liquid layer remains on it upon cyclically draining and replenishing the sample solution, ensuring an efficient mass transfer of an analyte onto a microarray. Prior to the signal detection procedure, disulfide bonds are chemically cleaved, and the perfluoroalkyl chains are removed from the microarray surface along with nonspecifically adsorbed proteins, resulting in extremely low background. Using conventional fluorescent detection, we show a 30-fold increase in signal/background ratio compared to a common epoxy-modified glass substrate. The combination of this technique with magnetic beads detection results in a simple and ultrasensitive cholera toxin (CT) immunoassay. The limit of detection (LOD) is 1 fM, which is achieved with an analyte binding time of 1 h. Efficient mass transfer provides highly sensitive detection of whole virus particles despite their low diffusion coefficient. The achieved LOD for vaccinia virus is 10 4 particles in 1 mL of sample. Finally, we have performed for the first time the simultaneous detection of whole virus and CT protein biomarker in a single assay. The developed technique can be used for multiplex detection of trace amounts of pathogens of various natures.

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“…42 A rapid and sensitive method to detect smallpox virus has been developed for use at point of care, based on antibody immuno column for analytical processes (ABICAP) immunofiltration, that produces results in about 45 minutes. 43 However, diagnostic electron microscopy is also still considered a fast and efficient method 44 to identify smallpox and other viral agents. Ebola virus was rapidly sequenced during the outbreak in Sierra Leone to link sporadic cases with the transmission chains.…”

Section: Diagnosis Of Disease Caused By Bioterrorist Agentsmentioning

confidence: 99%

Confronting the threat of bioterrorism: realities, challenges, and defensive strategies

Green

LeDuc

Cohen

et al. 2019

The Lancet Infectious Diseases

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Global terrorism is a rapidly growing threat to world security, and increases the risk of bioterrorism. In this Review, we discuss the potential threat of bioterrorism, agents that could be exploited, and recent developments in technologies and policy for detecting and controlling epidemics that have been initiated intentionally. The local and international response to infectious disease epidemics, such as the severe acute respiratory syndrome and west African Ebola virus epidemic, revealed serious shortcomings which bioterrorists might exploit when intentionally initiating an epidemic. Development of new vaccines and antimicrobial therapies remains a priority, including the need to expedite clinical trials using new methodologies. Better means to protect health-care workers operating in dangerous environments are also needed, particularly in areas with poor infrastructure. New and improved approaches should be developed for surveillance, early detection, response, effective isolation of patients, control of the movement of potentially infected people, and risk communication. Access to dangerous pathogens should be appropriately regulated, without reducing progress in the development of countermeasures. We conclude that preparedness for intentional outbreaks has the important added value of strengthening preparedness for natural epidemics, and vice versa. This is the second in a Series of three papers about terrorism and health in collaboration with The Lancet Psychiatry

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Rapid and sensitive point-of-care detection of Orthopoxviruses by ABICAP immunofiltration

Cited by 32 publications

References 21 publications

Sensitive and rapid detection of cholera toxin subunit B using magnetic frequency mixing detection

Sensitive and rapid detection of cholera toxin subunit B using magnetic frequency mixing detection

Improving Immunoassay Performance with Cleavable Blocking of Microarrays

Confronting the threat of bioterrorism: realities, challenges, and defensive strategies

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