Rapid and chronological expression of angiogenetic genes is a major mechanism involved in cell sheet transplantation in a rat gastric ulcer model

Yamaguchi, Shun; Higashi, Miki; Kanetaka, Kengo; Maruya, Yasuhiro; Kobayashi, Shinichiro; Hashiguchi, Keiichi; Hidaka, Masaaki; Nakao, Kazuwa; Eguchi, Susumu

doi:10.1016/j.reth.2022.08.008

Cited by 2 publications

(1 citation statement)

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“…The secretion of various growth factors, such as hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF), can locally promote angiogenesis at the transplanted site. We also recently indicated that myoblast sheet transplantation accelerates healing of the transplanted site through the chronological expression of various growth factors [ 34 ]. Based on the above findings, one of the mechanisms of cell sheet transplantation may therefore directly promote the regeneration of a thinned duodenal wall, and thereby strengthen the adhesion around the ESD site.…”

Section: Discussionmentioning

confidence: 99%

A first-in-human clinical study of laparoscopic autologous myoblast sheet transplantation to prevent delayed perforation after duodenal endoscopic mucosal dissection

Kanetaka,

Maruya,

Higashi

et al. 2024

Stem Cell Res Ther

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Background The detection rate of superficial non-ampullary duodenal epithelial tumors (SNADETs) has recently been increasing. Large tumors may contain malignant lesions and early therapeutic intervention is recommended. Endoscopic mucosal dissection (ESD) is considered a feasible treatment modality, however, the anatomical and physiological characteristics of the duodenum create a risk of postoperative perforation after ESD. Methods To explore whether myoblast sheet transplantation could prevent delayed perforation after ESD, a first-in-human (FIH) clinical trial of laparoscopic autologous myoblast sheet transplantation after duodenal ESD was launched. Autologous myoblast sheets fabricated from muscle tissue obtained seven weeks before ESD were transplanted laparoscopically onto the serous side of the ESD. The primary endpoints were the onset of peritonitis due to delayed perforation within three days after surgery and all adverse events during the follow-up period. Results Three patients with SNADETs ≥ 20 mm in size underwent transplantation of a myoblast sheet onto the serous side of the duodenum after ESD. In case 1, The patient’s postoperative course was uneventful. Endoscopy and abdominal computed tomography revealed no signs of delayed perforation. Despite incomplete mucosal closure in case 2, and multiple micro perforations during ESD in case 3, cell sheet transplantation could prevent the postoperative massive perforation after ESD, and endoscopy on day 49 after transplantation revealed no stenosis. Conclusions This clinical trial showed the safety, efficacy, and procedural operability of this novel regenerative medicine approach involving transplanting an autologous myoblast sheet laparoscopically onto the serosa after ESD in cases with a high risk of delayed perforation. This result indicates the potential application of cell sheet medicine in treating various abdominal organs and conditions with minimal invasiveness in the future. Trial registration jRCT, jRCT2073210094. Registered November 8 2021, https://jrct.niph.go.jp/latest-detail/jRCT2073210094.

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Section: Discussionmentioning

confidence: 99%