Rapid Analysis of Cyclic Peptide Cyclosporine A by HPLC Using a Column Packed with Nonporous Particles

Abstract

We developed a rapid and efficient analytical technique for cyclosporine A using HPLC on a column packed with 2-µm nonporous octadecylsilyl silica particles. Under optimized conditions, cyclosporine A was separated with high resolution from other cyclic peptides within 3 min, because the mass transfer resistance in the stationary phase was reduced by the use of the small, nonporous particles. Although the plate number increased greatly with the increase in the column temperature, the retention times were not affected. This behavior is different from other cyclic peptides or linear peptides. Based on its physicochemical characteristics, cyclosporine A is a poor hydrogen bond donor, and has a small topological polar surface area, low rotatable bond count, and high log P value. These results show that cyclosporine A is structurally rigid and undergoes poor water solvation even at high temperature. In the context of the rapid development of cyclic peptides with similar physicochemical characteristics to cyclosporine A, our developed method is useful for the development of cyclic peptide therapeutics.

Retention Behavior of Various Aromatic Compounds on Poly(butylene terephthalate) Stationary Phase in Liquid Chromatography

“…In order to improve the separation process, various types of chemically-bonded stationary phases have been developed during the past several decades [15][16][17][18][19][20], and octadecylsilica (ODS) phases are the most dominant stationary phase in LC [21][22][23][24] due to the good separation performance and the wide availability.…”

Fibrous Polyimide Stationary Phase for the Separation of Aromatic Compounds in Reversed-Phase Liquid Chromatography

“…In order to achieve effective separation for various types of complex sample mixtures in LC measurements, appropriate separation conditions must be chosen. In particular, an appropriate choice of stationary phase is quite important [8][9][10][11][12]. Octadecylsilica (ODS) is the most popular stationary phase in reversed-phase (RP) LC owing to its good separation performance for various complex sample mixtures [13][14][15][16][17][18][19].…”