Rapamycin/DiR loaded lipid-polyaniline nanoparticles for dual-modal imaging guided enhanced photothermal and antiangiogenic combination therapy

Abstract

Imaging-guided photothermal therapy (PTT) has promising application for treating tumors. Nevertheless, so far imaging-guided photothermal drug-delivery systems have been developed with limited success for tumor chemo-photothermal therapy. In this study, as the proof-of-concept, a stimuli-responsive tumor-targeting rapamycin/DiR loaded lipid-polyaniline nanoparticle (RDLPNP) for dual-modal imaging-guided enhanced PTT efficacy is reported for the first time. In this system, polyaniline (PANI) with π-π electronic conjugated system and effective photothermal efficiency is chosen as the appropriate model receptor of fluorescence resonance energy transfer (FRET), and loaded cyanine probe (e.g., 1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide, DiR) acts as the donor of near-infrared fluorescence (NIRF). In addition, rapamycin (RAPA), which is used as the antiangiogenesis chemotherapeutic drug, can cutdown the tumor vessels and delay tumor growth obviously. After intravenous treatment of RDLPNPs into Hela tumor bearing mice, fluorescent (from DiR) and enhanced photoacoustic (from DLPNPs) signals were found in tumor site over time, which reached to peak at the 6h time point. After irradiating with an NIR laser, a good anti-tumor effect was observed owing to the enhanced photothermal and antiangiogenic effect of RDLPNPs. These results show that the multifunctional nanoparticle can be used as a promising imaging-guided photothermal drug delivery nanoplatform for cancer therapy.

Preparation and antitumor evaluation of self-assembling oleanolic acid-loaded Pluronic P105/D-α-tocopheryl polyethylene glycol succinate mixed micelles for non-small-cell lung cancer treatment

“…DiR has been developed as a popular fluorescent dye with strong absorption in the nearinfrared region and is almost nontoxic at low concentration, making it suitable for in vivo fluorescence imaging. 38,39 After intravenous injection, DiR-loaded micelles are observed in A549 tumor xenografts. Mice treated with DiR-micelles showed a certain time-dependent increase in the fluorescence signals, especially at 24 h after injection ( Figure 5).…”

Iron Oxide Nanoparticles: Innovative Tool in Cancer Diagnosis and Therapy

“…Although IONPs present uncountable ways for cancer treatment and diagnosis, solid lipid particles should also be mentioned as powerful perspective tool in cancer therapy. Typically, photosensitizer such as phthalocyanine or temoporfin noncovalently bounded on the surface of solid lipid nanoparticles presents novel type of anticancer therapy, however, solid lipid nanoparticles are also appropriate for photothermal therapy using, for example, rapamycin loaded lipid‐polyaniline nanoparticle or simply they are perspective as targeted delivery system of anticancer drugs including paclitaxel, doxorubicin, or camptothecin . Even though solid lipid nanoparticles show excellent results in cancer therapy, IONPs enable more variable use as both therapeutic and diagnostic tool due to their unique magnetic and physico‐chemical properties and also as it will be described below their multifunctional application is investigated.…”

A Laser-Activated Biocompatible Theranostic Nanoagent for Targeted Multimodal Imaging and Photothermal Therapy

“…Due to their excellent biocompatibility and drug loading capacity, polymer [11,14,17], liposome [18] and protein [19][20][21] materials have been explored as theranostic agents. Through encapsulation with optical absorbers, these biocompatible materials integrate multimodal imaging and PTT technology, simultaneously providing therapeutic function and diagnostic imaging information all in one.…”