RAP1 protein interacts with yeast telomeres in vivo: Overproduction alters telomere structure and decreases chromosome stability

Cancer and aging: the importance of telomeres in genome maintenance

“…Thus, TIN2 may link the functions of TRF1 and TRF2. In addition to binding TIN2, TRF2 also binds RAP1 [61], the mammalian homologue of the yeast direct telomere binding proteins Rap1p (Saccharomyces cerevisiae) [62]and Taz1 (Schizosaccharomyces pombe) [63]. However, in contrast to Rap1p and Taz1, human RAP1 does not bind telomeric DNA directly, but rather associates with telomeres indirectly by binding TRF2 (Fig.…”

High copy number suppression of the meiotic arrest caused by a dmc1 mutation: REC114 imposes an early recombination block and RAD54 promotes a DMC1‐independent DSB repair pathway

“…The sequence of approximately 100 nucleotides from each end of the insert was determined and used to search the Stanford Yeast Genome Database (SGD; http://genome-www.stanford.edu/Saccharomyces/). Sequence information was used to subclone smaller open reading frame-containing fragments into the very high copy number vector YepFAT4 (Conrad et al 1990;K. Runge, personal communication).…”

Human telomeres are attached to the nuclear matrix.

“…In Oxytricha and Euplotes, the protruding G-rich repeats are bound by a telomere capping complex which protects telomeres from ligation and exonucleolytic attack in vitro (Lipps et al, 1982;Gottschling and Cech, 1984;Gottsc.hling and Zakian, 1986;Price andCech, 1987, 1989;Raghuraman et al, 1989;Price, 1990). Telomere capping complexes have not been identified in other organisms, but genetic and biochemical experiments suggest that the telomeres of Saccharomyces cerevisiae interact with the repressor/activator protein RAPI (Berman et al, 1986;Buchman et al, 1988;Longtine et al, 1989;Conrad et al, 1990;Lustig et al, 1990). The function of the RAPI -telomere association is not known.…”