Ranbpm homologue genes characterised in the cyst nematodes Globodera pallida and Globodera ‘mexicana’

Blanchard, Alexandra; Esquibet, Magali; Fouville, Didier; Grenier, Éric

doi:10.1016/j.pmpp.2005.09.001

Cited by 29 publications

(34 citation statements)

References 20 publications

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“…The Gp -RBP-1 sequences deduced from these populations showed a number of insertion/deletion polymorphisms and amino acid substitutions (Figure 3). Most notably, Chav-6 and Rook-3 showed a 15 aa insertion that is highly similar in length and sequence to that encoded by Gp-Rbp-1 intron 3 (44 bp in length) [18]. Thus, some Gp -RBP-1 isoforms may be expressed by alternative splicing although the possibility that these clones represent different alleles of the same gene or different gene copies cannot be discounted.…”

Section: Resultsmentioning

confidence: 99%

The Cyst Nematode SPRYSEC Protein RBP-1 Elicits Gpa2- and RanGAP2-Dependent Plant Cell Death

et al. 2009

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Plant NB-LRR proteins confer robust protection against microbes and metazoan parasites by recognizing pathogen-derived avirulence (Avr) proteins that are delivered to the host cytoplasm. Microbial Avr proteins usually function as virulence factors in compatible interactions; however, little is known about the types of metazoan proteins recognized by NB-LRR proteins and their relationship with virulence. In this report, we demonstrate that the secreted protein RBP-1 from the potato cyst nematode Globodera pallida elicits defense responses, including cell death typical of a hypersensitive response (HR), through the NB-LRR protein Gpa2. Gp-Rbp-1 variants from G. pallida populations both virulent and avirulent to Gpa2 demonstrated a high degree of polymorphism, with positive selection detected at numerous sites. All Gp-RBP-1 protein variants from an avirulent population were recognized by Gpa2, whereas virulent populations possessed Gp-RBP-1 protein variants both recognized and non-recognized by Gpa2. Recognition of Gp-RBP-1 by Gpa2 correlated to a single amino acid polymorphism at position 187 in the Gp-RBP-1 SPRY domain. Gp-RBP-1 expressed from Potato virus X elicited Gpa2-mediated defenses that required Ran GTPase-activating protein 2 (RanGAP2), a protein known to interact with the Gpa2 N terminus. Tethering RanGAP2 and Gp-RBP-1 variants via fusion proteins resulted in an enhancement of Gpa2-mediated responses. However, activation of Gpa2 was still dependent on the recognition specificity conferred by amino acid 187 and the Gpa2 LRR domain. These results suggest a two-tiered process wherein RanGAP2 mediates an initial interaction with pathogen-delivered Gp-RBP-1 proteins but where the Gpa2 LRR determines which of these interactions will be productive.

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Section: Resultsmentioning

confidence: 99%

The Cyst Nematode SPRYSEC Protein RBP-1 Elicits Gpa2- and RanGAP2-Dependent Plant Cell Death

et al. 2009

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“…(43) In addition, cyst nematodes secrete proteins that may manipulate or exploit ubiquitination pathways in hosts (44) as well as a large family of proteins similar to RanBPMs. (45) From the above, it is clear that PCN and pRKN have some aspects of parasitism in common and some aspects that differ and, as might be expected, some parasitism proteins are common to both nematode groups while others are specifically associated with PCN or pRKN. Given the presence of cell-walldegrading enzymes in both nematode groups and the likely acquisition of such genes from bacteria, it is tempting to speculate that the ability to digest plant cell walls is a prerequisite for plant parasitism and that an ancestor of the entire group acquired these genes as a first step in the evolution of the complex parasitic interactions that are present today.…”

Section: Nematode Proteins Involved In Parasitismmentioning

confidence: 81%

Parasitism genes and host range disparities in biotrophic nematodes: the conundrum of polyphagy versus specialisation

Blok¹,

Jones²,

Phillips³

et al. 2008

BioEssays

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This essay considers biotrophic cyst and root-knot nematodes in relation to their biology, host-parasite interactions and molecular genetics. These nematodes have to face the biological consequences of the physical constraints imposed by the soil environment in which they live while their hosts inhabit both above and below ground environments. The two groups of nematodes appear to have adopted radically different solutions to these problems with the result that one group is a host specialist and reproduces sexually while the other has an enormous host range and reproduces by mitotic parthenogenesis. We consider what is known about the modes of parasitism used by these nematodes and how it relates to their host range, including the surprising finding that parasitism genes in both nematode groups have been recruited from bacteria. The nuclear and mitochondrial genomes of these two nematode groups are very different and we consider how these findings relate to the biology of the organisms.

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“…Hücresel düzenleyici olarak görev yapan ve küçük G-protein Ran'a bağlanan proteinlere çok benzeyen ve RanBPM olarak adlandırılan salgı proteinleri kist nematodlarının dorsal özofagus bez hücrelerinde belirlenmiştir (Blanchard et. al., 2005;Qin et.…”

Section: Ran-binding Protein M (Ranbpm)unclassified

Nematode parasitism enzymes and their functions

Devran

Mıstanoğlu

2016

Türkiye Entomoloji Bülteni

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Plant parasitic nematodes are important agricultural pest groups. They establish parasitic interaction with their host during feeding period. The secretions called parasitism enzymes produced by nematodes play an important role in hostnematode interaction. The secretions have many properties such as degradation of plant cell wall, cellular metabolism and transport, cellular regulations and targeting, and mitigating host response. Recently, researches focus on functions of the enzymes produced by nematodes for plant-nematode interaction in the cellular and molecular level. The aim of the article is to give information about important nematode parasitism enzymes and their secretion locations.

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Ranbpm homologue genes characterised in the cyst nematodes Globodera pallida and Globodera ‘mexicana’

Cited by 29 publications

References 20 publications

The Cyst Nematode SPRYSEC Protein RBP-1 Elicits Gpa2- and RanGAP2-Dependent Plant Cell Death

The Cyst Nematode SPRYSEC Protein RBP-1 Elicits Gpa2- and RanGAP2-Dependent Plant Cell Death

Parasitism genes and host range disparities in biotrophic nematodes: the conundrum of polyphagy versus specialisation

Nematode parasitism enzymes and their functions

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