Raman spectroscopy of saliva as a perspective method for periodontitis diagnostics

Гончуков, С. А.; Sukhinina, A. V.; Bakhmutov, D; Минаева, С. А.

doi:10.1002/lapl.201110095

Cited by 59 publications

(55 citation statements)

References 44 publications

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“…Its recent spectacular awakening has brought it at the forefront of modern biochemical science (Baena & Lendl, ). In reviewing recent publications on biomolecules, it becomes clear how Raman spectroscopy has proved invaluable in unveiling a variety of issues in a number of key fields of microbiology, including metabolism of cells (M. Li, Romero‐Gonzales, Banwart, & Huang, ; Notingher, ; Notingher, Verrier, Haque, Polak, & Hench, ; Smith, Wright, & Ashton, ), bacteria (Gonchukov, Sukhinina, Bakhmutov, & Minaeva, ; Lorenz, Wichmann, Stockel, Rosch, & Popp, ; Novelli‐Rousseau et al, ; Strola et al, ), and viruses (Otange, Birech, Okonda, & Rop, ; Tuma & Thomas, ). The so far reported research also holds a promise for more accurate, fully quantitative, and rapid microbiological identifications of crucial issues in clinical diagnostics.…”

Section: Introductionmentioning

confidence: 99%

Metabolic machinery encrypted in the Raman spectrum of influenza A virus‐inoculated mammalian cells

Pezzotti

Zhu

Adachi

et al. 2019

Journal Cellular Physiology

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Raman spectroscopy was applied with a high spectral resolution to a structural study of Influenza (type A) virus before and after its inoculation into Madin–Darby canine kidney cells. This study exploits the fact that the major virus and cell constituents, namely DNA/RNA, lipid, and protein molecules, exhibit peculiar fingerprints in the Raman spectrum, which clearly differed between cells and viruses, as well as before and after virus inoculation into cells. These vibrational features, which allowed us to discuss viral assembly, membrane lipid evolution, and nucleoprotein interactions of the virus with the host cells, reflected the ability of the virus to alter host cells’ pathways to enhance its replication efficiency. Upon comparing Raman signals from the host cells before and after virus inoculation, we were also able to discuss in detail cell metabolic reactions against the presence of the virus in terms of compositional variations of lipid species, the formation of fatty acids, dephosphorylation of high‐energy adenosine triphosphate molecules, and enzymatic hydrolysis of the hemagglutinin glycoprotein.

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Section: Introductionmentioning

confidence: 99%

Metabolic machinery encrypted in the Raman spectrum of influenza A virus‐inoculated mammalian cells

Pezzotti

Zhu

Adachi

et al. 2019

Journal Cellular Physiology

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“…Vibrational spectroscopies, such as Raman and infrared spectroscopies, can be effectively useful to analyze biological samples [9]. In particular, Raman spectroscopy is not affected by water presence and this is highly desirable in biological tissue analysis [10][11][12]. This technique was largely adopted for investigations on the conformational changes of the peptide backbone of protein fibers [13,14].…”

Section: Introductionmentioning

confidence: 99%

“…Stress application on chemical bonds determines some modifications in interatomic distances and consequently in the position of Raman bands due to the anharmonicity of the vibrational energy [10]. In recent years, micro-Raman spectroscopy (µ-RS) emerged as a non-destructive, non-invasive, very sensitive, and less time-consuming methodology for analyzing biological specimens, usually requiring simple or no special sample preparation when compared to traditional assays [12,[15][16][17][18]. In our previous studies, a preliminary in vitro investigation on bovine PDL was performed to identify the most suitable sample-analysis experiment conditions [19].…”

Section: Introductionmentioning

confidence: 99%

Monitoring Biochemical and Structural Changes in Human Periodontal Ligaments during Orthodontic Treatment by Means of Micro-Raman Spectroscopy

Perillo

d’Apuzzo

Illario

et al. 2020

Sensors

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The aim of the study was to examine the biochemical and structural changes occurring in the periodontal ligament (PDL) during orthodontic-force application using micro-Raman spectroscopy ( μ -RS). Adolescent and young patients who needed orthodontic treatment with first premolar extractions were recruited. Before extractions, orthodontic forces were applied using a closed-coil spring that was positioned between the molar and premolar. Patients were randomly divided into three groups, whose extractions were performed after 2, 7, and 14 days of force application. From the extracted premolars, PDL samples were obtained, and a fixation procedure with paraformaldehyde was adopted. Raman spectra were acquired for each PDL sample in the range of 1000–3200 cm − 1 and the more relevant vibrational modes of proteins (Amide I and Amide III bands) and CH 2 and CH 3 modes were shown. Analysis indicated that the protein structure in the PDL samples after different time points of orthodontic-force application was modified. In addition, changes were observed in the CH 2 and CH 3 high wavenumber region due to local hypoxia and mechanical force transduction. The reported results indicated that μ -RS provides a valuable tool for investigating molecular interchain interactions and conformational modifications in periodontal fibers after orthodontic tooth movement, providing quantitative insight of time occurring for PDL molecular readjustment.

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“…Рамановское рассеяние применяется для диагностики периодонтита по анализу слюны [2,3], а также для выявления кариеса при изменении состава и структуры эмали [4][5][6]. Недостатками этого метода является использование преимущественно в условиях ex vivo, а также при наличии уже сформированного поражения зубной эмали.…”

Section: Introductionunclassified

Aluminum phthalocyanine nanoparticles activation for local fluorescence spectroscopy in dentistry

Золотарева

Farrakhova

Куприянова

et al. 2018

BMP

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Early diagnosis of caries and tooth enamel microcracks is of great importance for preventing the destruction of healthy tooth enamel. Inorder to detect microcracks in the enamel and pathogenic microflora foci that can cause caries, nanoform of aluminum phthalocyanine (AlPc) can be used as a marker. In a colloidal solution, the nanoparticles do not fluoresce, unlike their molecular form. To convert the particle into its molecular form, it is necessary to have a solvent or specific environment (bacteria, macrophages, etc.). That is why the hydrophobic nanoparticles of aluminum phthalocyanine (nAlPc) can act as markers for detecting hidden pathogenic microflora during fluorescent diagnostics. Further reduction of the diagnosis time and increase the efficiency can be achieved by using biologically compatible surfactants as additional activators of nAlPc.In order to carry out local fluorescence spectroscopy of enamel microcracks and pathogenic microflora foci on the enamel surface, a model compound containing surfactants, auxiliary components and nAlPc colloid at a concentration of 10 mg/l was prepared.Studies on the interaction of the model compound with nAlPc and Protelan MST-35 with tooth enamel ex vivo have shown this surfactant to be a promising auxiliary activator of the nanoparticles, allowing conducting local fluorescence spectroscopy of the tooth enamel surface 3 min after application. In addition, statistical processing of the results showed the effectiveness of using the model compound for local fluorescence spectroscopy of the enamel surface in order to detect the enamel microcracks and the pathogenic microflora accumulation foci that can lead to the development of a cariogenic process.

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Raman spectroscopy of saliva as a perspective method for periodontitis diagnostics

Cited by 59 publications

References 44 publications

Metabolic machinery encrypted in the Raman spectrum of influenza A virus‐inoculated mammalian cells

Metabolic machinery encrypted in the Raman spectrum of influenza A virus‐inoculated mammalian cells

Monitoring Biochemical and Structural Changes in Human Periodontal Ligaments during Orthodontic Treatment by Means of Micro-Raman Spectroscopy

Aluminum phthalocyanine nanoparticles activation for local fluorescence spectroscopy in dentistry

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