Raman microprobe and microscope with laser excitation

Delhaye, M.; Dhamelincourt, P.

doi:10.1002/jrs.1250030105

Cited by 431 publications

(133 citation statements)

References 5 publications

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“…With the advent of the Raman microprobe and mapping/ imaging techniques pioneered by Rosasco et al (1975) and Delhaye and Dhamelincourt (1975), Raman microspectroscopy and hyperspectral imaging have become established and routinely used in the geosciences.…”

Section: Raman Spectroscopy and Hyperspectral Imagingmentioning

confidence: 99%

Raman Hyperspectral Imaging of Microfossils: Potential Pitfalls

Marshall

Marshall²

2013

Astrobiology

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Initially, Raman spectroscopy was a specialized technique used by vibrational spectroscopists; however, due to rapid advancements in instrumentation and imaging techniques over the last few decades, Raman spectrometers are widely available at many institutions, allowing Raman spectroscopy to become a widespread analytical tool in mineralogy and other geological sciences. Hyperspectral imaging, in particular, has become popular due to the fact that Raman spectroscopy can quickly delineate crystallographic and compositional differences in 2-D and 3-D at the micron scale. Although this rapid growth of applications to the Earth sciences has provided great insight across the geological sciences, the ease of application as the instruments become increasingly automated combined with nonspecialists using this techique has resulted in the propagation of errors and misunderstandings throughout the field. For example, the literature now includes misassigned vibration modes, inappropriate spectral processing techniques, confocal depth of laser penetration incorrectly estimated into opaque crystalline solids, and a misconstrued understanding of the anisotropic nature of sp 2 carbons. Key Words: Raman spectroscopy-Raman imaging-Confocal Raman spectroscopy-Disordered sp 2 carbons-HematiteMicrofossils. Astrobiology 13, 920-931.

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Section: Raman Spectroscopy and Hyperspectral Imagingmentioning

confidence: 99%

Raman Hyperspectral Imaging of Microfossils: Potential Pitfalls

Marshall

Marshall²

2013

Astrobiology

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“…Soon thereafter, lasers were used to drive Raman scattering [5,6] and the number of applications increased rapidly, particularly in the analysis of biomolecules. Other important developments accelerating the progress of Raman spectroscopy include the digitization of spectra using charge-coupled devices (CCDs) [7,8], the confocal Raman microscope [9], and improved filters to remove light at the laser wavelength [10]. These inventions allowed a rapid increase in the popularity of using Raman to study biological samples in the early 1990s [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

255

189

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Raman spectroscopy is an increasingly popular technique in many areas including biology and medicine.It is based on Raman scattering, a phenomenon in which incident photons lose or gain energy via interactions with vibrating molecules in a sample. These energy shifts can be used to obtain information regarding molecular composition of the sample with very high accuracy. Applications of Raman spectroscopy in the life sciences have included quantification of biomolecules, hyperspectral molecular imaging of cells and tissue, medical diagnosis, and others. This review briefly presents the physical origin of Raman scattering explaining the key classical and quantum mechanical concepts. Variations of the Raman effect will also be considered, including resonance, coherent, and enhanced Raman scattering. We discuss the molecular origins of prominent bands often found in the Raman spectra of biological samples. Finally, we examine several variations of Raman spectroscopy techniques in practice, looking at their applications, strengths, and challenges. This review is intended to be a starting resource for scientists new to Raman spectroscopy, providing theoretical background and practical examples as the foundation for further study and exploration.

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“…Standard laser scanning based Raman spectroscopic imaging [13,14] allows the quantification of the chemical heterogeneity of a sample in terms of the spatial distribution of its molecular constituents. For these reasons, Raman imaging applications cover a wide range of scientific disciplines such as, pharmacology, biology, medicine and material science [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Nonredundant Raman imaging using optical eigenmodes

Kosmeier¹,

Zolotovskaya²,

Luca³

et al. 2014

Optica

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Various forms of imaging schemes have emerged over the last decade that are based on correlating variations in incident illuminating light fields to the outputs of single 'bucket' detectors. However, to date, the role of the orthogonality of the illumination fields has largely been overlooked and furthermore, the field has not progressed beyond bright field imaging. By exploiting the concept of orthogonal illuminating fields, we demonstrate the application of optical eigenmodes (OEi) to wide field, scan-free spontaneous Raman imaging, which is notoriously slow in wide-field mode. The OEi approach enables a form of indirect imaging that exploits both phase and amplitude in image reconstruction. The use of orthogonality enables us to non-redundantly illuminate the sample and, in particular, use a subset of illuminating modes to obtain the majority of information from the sample, thus minimising any photobleaching or damage of the sample. The crucial incorporation of phase, in addition to amplitude, in the imaging process significantly reduces background noise and results in an improved SNR for the image while reducing the number of illuminations. As an example we can reconstruct images of a surface-enhanced Raman spectroscopy (SERS) active sample with approximately an order of magnitude fewer acquisitions. This generic approach may readily be applied to other imaging modalities such as fluorescence microscopy or nonlinear vibrational microscopy.

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Raman microprobe and microscope with laser excitation

Cited by 431 publications

References 5 publications

Raman Hyperspectral Imaging of Microfossils: Potential Pitfalls

Raman Hyperspectral Imaging of Microfossils: Potential Pitfalls

Raman spectroscopy: techniques and applications in the life sciences

Nonredundant Raman imaging using optical eigenmodes

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