Raffinose and hypotaurine improve the post-thawed Merino ram sperm parameters

Effect of Antioxidants and Apoptosis Inhibitors on Cryopreservation of Murine Germ Cells Enriched for Spermatogonial Stem Cells

“…ATP usage was evaluated because previous work has reported that the mechanism of action of hypotaurine as a cryoprotectant is to increase mitochondrial activity, presumably improving viability and integrity of the cell [31, 32]. In the present study, the effects of various cryoprotective agents on mitochondrial activity mirrored the results of the proliferation analysis.…”

Effect of crocin and naringenin supplementation in cryopreservation medium on post-thawed rooster sperm quality and expression of apoptosis associated genes

“…Adding antioxidant compounds to the freezing medium is known as one of the ways to defeat the deleterious effects of ROS on sperm fertility after thawing, because it blocks or inhibits oxidative stress. Antioxidants provide a positive effect on semen, leading to an improvement in some sperm parameters containing motility and membrane integrity [11–13].…”

Current status and advances in ram semen cryopreservation

“…Lyophilized or fresh egg yolk Similar cryoprotective effects Alcay et al, 2015 Natural or lyophilized low density lipoproteins Total and progressive motility Moustacas et al, 2011 Casein, palm oil, coconut oil or egg yolk Egg yolk is superior Del Valle et al, 2013 Different species' egg yolk Sperm quality by 15% chucker egg yolk Kulaksiz et al, 2010 -Egg yolk concentration -Bioexcell ® or egg yolk -Optimal concentration: 5-10% -Bioexcell ® may be an alternative Gil et al, 2003 Soybean concentrations between 1-1.5% Motility, viability, membrane integrity - Emamverdi et al, 2013-Forouzanfar et al, 2010 Soybean lecithin or egg yolk 1% soybean lecithin similar with 20% egg yolk extenders Masoudi et al, 2016 Soybean lecithin concentration -Soy lecithin > 1% : semen quality -Soy lecithin > 3.5% : no significant effect on motility -Soy lecithin 3.5% : mitochondrial activity One-step or at 5° C two-steps glycerol addition No effect on motility and viability Morrier et al, 2002 4% glycerol in a two-step dilution (to 2% at 35 o C and to 4% at 5 o C) Good field fertility rate Anel et al, 2003 Osmolality of glycerol based extenders in a two-step dilution High osmotic pressure (400 mOsm) Soylu et al, 2007 Glycerol alone or in combination with trehalose 5% glycerol and 100 mM trehalose: post-thawing sperm quality Najafi et al, 2013 -100 mM trehalose with 5% glycerol -100 mM trehalose and 8% LDL High post-thawing semen quality - Jafaroghli et al, 2011-Tonieto et al, 2010 Trehalose in combination with fructose Motility and viability Matsuoka et al, 2006 Trehalose, sucrose, raffinose, hypotaurine High ram semen cryo-protection - Bucak et al, 2013-Jafaroghli et al, 2011 Cyclodextrins pre-loaded with cholesterol Binding capacity to zona pellucida Mocé et al, 2010 Seminal plasma -Semen quality and fertility rate -No beneficial effect on sperm quality Uysal and Bucak (2007) observed that the addition of lycopene, a natural carotenoid which occurs in certain fruits and vegetables, in a Tris-based extender exhibited high protection of all tested ram semen parameters after thawing. Similar results were obtained by the supplementation of soybean lecithin extender with 4 or 6% rosemary aqueous extract (Motlagh et al, 2014).…”

“…Similar findings were provided by Tonieto et al (2010) by the supplementation of Trisegg yolk-glycerol extender with100 mM trehalose and 8% LDL, while Matsuoka et al (2006) noticed that the addition of trehalose to an egg-yolk based ram semen diluent containing fructose, improves post-thaw sperm motility and viability. Although trehalose is the most investigated sugar additive in semen extenders, other sugars such as sucrose, raffinose and hypotaurine also resulted in higher ram semen cryoprotection (Bucak et al, 2013;Jafaroghli et al, 2011). Furthermore, a beneficial role of cyclodextrins, a family of cyclic oligosaccharides with a hydrophilic outer surface and a lipophilic central cavity, on semen has been found by Mocé et al (2010).…”