Radioprotective profile of Urtica dioica L. seed extract on oxidative DNA-damage in liver tissue and whole blood of radiation-administered rats

Abstract

It was aimed to investigate the radioprotective activity of Urtica dioica L. seed extract (UDSE) in the whole blood and liver of radiation-administered rats, both biochemically and immunohistochemically. 32 rats were divided into 4 groups (n:8). Control group (C): no administration for 10 days. Radiation group (IR): fed pellets for 10 days after exposure to radiation. Radiation + UDSE (IR+UDSE) group: exposed to radiation and fed UDSE for 10 days. UDSE group (UDSE): fed UDSE for 10 days. Radiation (5Gy) was given as a single fraction. 8-hydroxy-2-deoxyguanosine (8-OHdG) and deoxyguanosine (dG) levels were analyzed by biochemical method and glutathione peroxidase 1 (GPx-1) analyses were performed by immunohistochemical method in the liver and blood tissues of the rats. The increased 8-OHdG rates and decreased GPx-1 immunoreactivity was observed in the IR group. Those parameters were ameliorated in the IR+UDSE group when compared to the IR group. UDSE is likely to be a valuable radioprotector against the harmful effects of radiation.

Protective effects of Saraca indica L. leaves extract (family Fabaceae) against gamma irradiation induced injury in the kidney of female albino rats

“…The present depletion of antioxidant system accompanied by enhancement of lipid peroxides matches with the data reported by Ahlatcı et al 48 This result may be due to great consumption of the antioxidant system to cover the free radicals produced by radiation 49 …”

“…45 Pasupathi et al 46 48 This result may be due to great consumption of the antioxidant system to cover the free radicals produced by radiation. 49 Generally, imbalance between the content of pro-inflammatory mediators (eg, IL-1β, TNF-α, leptin) and anti-inflammatory mediators (eg, IL-10, adiponectin) generates inflammation process. 50 The rule of immune/inflammatory system is essential for keeping the equilibrium between protective and tissue-damaging responses.…”

Protective and Curative Effects of Fresh Orange Juice (Citrus Sinensis L.) Supplementation Against Liver Injuries, Hepatic Lipid, Protein, and Dnaoxidative Damage Induced Cyclooxygenase-2/Prostaglandin E2 Inflammatory Pathway in Female Irradiated Rats

“…On the other hand, antioxidant defense system capacity of the irradiated liver has decreased as a result of decreased hepatic GSH level as well as hepatic GSH-PX and SOD activities which are considered as strong liver antioxidants. Our results go hand in hand with Cheng et al [12,[25][26][27][28][29][30].…”

“…Our results go hand in hand with previous studies [12,[26][27][28][29][30], as a consequence of oxidative stress and inflammation of hepatic tissues, liver impairment and injury occurred and resulted in tissue degeneration resulting in increased liver's operational functioning enzymes (ALT, AST, and y-GT) in serum. That progressed into gallbladder disease indicated by increased ALP activity associated with jaundice (increased bilirubin level).…”