Radiolytic synthesis of OH group functionalized fullerene structures and their biosensor application

Abstract

In this study, radiolytic functionalization of fullerene in methanol/1,2-dichlorobenzene mixtures and its applications with respect to biosensor support materials were studied. To obtain supports for biosensors for electron transfer, fullerene was functionalized by c-irradiation in a methanol/1,2-dichlorobenzene mixture solution. The hydroxyl group-modified fullerene, F-fullerene, was characterized by Fourier-transform infrared, Raman spectroscopy, MALDI-TOF mass spectroscopy, and elemental analysis. As a result, the main hydroxyl group was successfully introduced on the surface of fullerene. F-fullerene was found to disperse well in water by ultrasonication. The results indicated that F-fullerene is a good candidate for use in biological systems as a biosensor support material. A biosensor based on F-fullerene was prepared by hand-casting the mixture of tyrosinase, F-fullerene, and 2% chitosan solution on an ITO electrode. Furthermore, the prepared biosensor was optimized pH and temperature. The prepared biosensor was then evaluated for its ability to analyze phenolic compounds contained in commercial red wines. The total phenolic concentration was determined to be in the range of 397-895 mg/L. From these results, the electron transfer ability of F-fullerene was improved on an enzyme biosensor.

Development of multifunctional polyurethane elastomer composites containing fullerene: Mechanical, damping, thermal, and flammability behaviors

“…Fullerene has been one of the most important carbonaceous nanomaterials of last 50 years because it possesses a unique zero-dimensional structure. Fullerenes and their derivatives have gained usage in polymeric composites with various applications, including photovoltaics, [1][2][3] voltage stabilizer, 4 nanosensors, 5,6 conductive materials, [7][8][9] and improvements of their tribological 10,11 and mechanical 12,13 properties. However, pristine fullerene particles tend to form aggregates in polymer matrices, which is the major problem that limits its proper applications.…”

Radiation-Assisted Synthesis of Polymer-Based Nanomaterials

“…Enhanced interaction between CNTs and polymer matrix help in uniform dispersion improving the electrical and mechanical properties of corresponding composites. Fullerene was shown to be functionalized with -OH groups by gamma irradiation in methanol solution for biosensor applications [6]. Water-soluble poly(acrylic acid) grafted multiwalled carbon nanotubes (MWCNTs) were prepared by two-step gamma irradiation [7].…”

Introduction of Various Amine Groups onto Poly(glycidyl methacrylate)-g-MWNTs and their Application as Biosensor Supports

“…This result indicates that the amine group on the electrode was an important factor in the immobilization of enzymes. In previous papers, 15,19,[22][23][24][25][26] tyrosinase immobilized-biosensors based on MWNT supports have also been prepared through the physical adsorption of tyrosinase onto electrodes supporting MWNTs with hydrophilic functional groups. In this case, a polymer binder, usually Nafion, was used to coat the MWNT supports with hydrophilic groups.…”