1984
DOI: 10.1210/jcem-59-2-269
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Abstract: Antisera prepared by immunization of rabbits with human T4-binding globulin (TBG) contained two populations of antibodies: one directed against determinants of the native molecule, and the other directed against antigenic sites present only in denatured TBG. These two populations of antibodies were present in all nine antisera prepared in this or other laboratories that were tested. Exploiting this property of anti-TBG sera and using radioiodinated denatured TBG as a tracer, a RIA was developed which measures … Show more

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Cited by 63 publications
(25 citation statements)
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“…Serum hormone levels for thyroglobulin (TG), thyroxine (T4) binding globulin (TBG), and TSH were determined by specific RIAs. Antithyroid microsomal antibodies (MCHA) and antithyroglobulin antibodies (TGHA) were measured by hemagglutination assay, and the free thyroxine index (FT41) was calculated as described (19)(20)(21)(22). T3, triiodothyronine.…”
Section: Methodsmentioning
confidence: 99%
“…Serum hormone levels for thyroglobulin (TG), thyroxine (T4) binding globulin (TBG), and TSH were determined by specific RIAs. Antithyroid microsomal antibodies (MCHA) and antithyroglobulin antibodies (TGHA) were measured by hemagglutination assay, and the free thyroxine index (FT41) was calculated as described (19)(20)(21)(22). T3, triiodothyronine.…”
Section: Methodsmentioning
confidence: 99%
“…The finding that carriers of TBG-Gary had elevated levels of denatured TBG (dnTBG) in serum, not usually encountered in association with thyroidal or nonthyroidal illnesses (15), led us to measure dnTBG in sera from 32 unrelated families with inherited TBG deficiency. High dnTBG levels were thus found in serum samples from 2 described (I15).…”
Section: Introductionmentioning
confidence: 99%
“…The tj/2 ofnTBG was calculated by the least-square analysis of the logarithm of residual nTBG concentrations and the corresponding times of incubation (10). The effect of added T4 on the heat denaturation of nTBG was studied as previously described (15).…”
Section: Introductionmentioning
confidence: 99%
“…However, this substance was not found by some workers [19]. Alternatively, the lower T4 levels might be explained by the increased desialylated TBG, which binds T4 with an avidity of about one-tenth that of native TBG ; it fully cross-reacts in native TBG assays [20,21], The efficient removal of desialylated TBG might result in higher T4 levels in CAPD patients. A previous study on the pH dependance of T4-TBG binding demonstrated that T4 was bound maximally and equally in the region of physiological pH, 6.8 to 7.7 [22,23], indicating that possi ble differences of serum pH could not be responsible for the higher T4 level in CAPD patients.…”
Section: Discussionmentioning
confidence: 86%