Survival curves were obtained for massive concentrations of spores of Clostridium botulinum strain 12885A, when subjected to a wide range of gamma radiation doses under various conditions. The spore concentrations ranged from 37,000 to 64,800,000,000 per ampoule, with no recovery at 5 Mrad or more. Only at lower doses were observed survival counts compatible with classical hit theory. More than one hit is apparently required to inactivate the Cl. botulinurn spore by gamma radiation. The number of hits …
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