Radiation Resistance of the Microflora Associated with Amniotic Membranes

Singh, Rita; Gupta, Payal; Purohit, Sumita; Kumar, Pawan; Vaijapurkar, S.G.; Chacharkar, M. P.

doi:10.1007/s11274-005-2890-8

Cited by 9 publications

(9 citation statements)

References 8 publications

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“…Different types of microorganisms have different dose response curves, and these differences imply that different microorganisms possess different innate sensitivities to radiation [8]. In our study, a total five isolates of Bacillus spp.…”

Section: Resultsmentioning

confidence: 97%

“…Furthermore, the amniotic membrane could be prepared rather inexpensively compared to the other biological dressings and possesses most of the characteristics of an ideal skin substitute [7]. As the membrane comes in contact with open wound, its microbiological quality is the most important consideration [8]. In order to avoid the transmission of diseases, the membrane needs be perfectly sterile and should bear high sterility assurance level (SAL).…”

Section: Introductionmentioning

confidence: 99%

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Effects of Gamma Irradiation on Bacterial Microflora Associated with Human Amniotic Membrane

Atique

Ahmed

Asaduzzaman

et al. 2013

BioMed Research International

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Human amniotic membrane is considered a promising allograft material for the treatment of ocular surface reconstruction, burns, and other skin defects. In order to avoid the transmission of any diseases, grafts should be perfectly sterile. Twenty-five amniotic sacs were collected to determine the microbiological quality of human amniotic membrane, to analyze the radiation sensitivity pattern of the microorganism, and to detect the radiation decimal reduction dose (D10) values. All the samples were found to be contaminated, and the bioburden was ranged from 3.4 × 102 to 1.2 × 105 cfu/g. Initially, a total fifty bacterial isolates were characterized according to their cultural, morphological, and biochemical characteristics and then tested for the radiation sensitivity in an incremental series of radiation doses from 1 to 10 KGy. The results depict gradual decline in bioburden with incline of radiation doses. Staphylococcus spp. were the most frequently isolated bacterial contaminant in tissue samples (44%). The D10 values of the bacterial isolates were ranged from 0.6 to 1.27 KGy. Streptococcus spp. were found to be the highest radioresistant strain with the radiation sterilization dose (RSD) of 11.4 KGy for a bioburden level of 1000. To compare the differences, D10 values were also calculated by graphical evaluations of the data with two of the representative isolates of each bacterial species which showed no significant variations. Findings of this study indicate that lower radiation dose is quite satisfactory for the sterilization of amniotic membrane grafts. Therefore, these findings would be helpful to predict the efficacy of radiation doses for the processing of amniotic membrane for various purposes.

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Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Effects of Gamma Irradiation on Bacterial Microflora Associated with Human Amniotic Membrane

Atique

Ahmed

Asaduzzaman

et al. 2013

BioMed Research International

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“…Kowalski et al [12] carried out assessment of bioburden on tissue from 101 human donors and observed bioburden ranging up to > 28000 CFU. A number of studies have reported bacterial contamination of amniotic membrane [13,14] . The most prevalent organisms were Staphylococci species.…”

Section: Methods For Sterilization Of Tissue Allograftsmentioning

confidence: 99%

Radiation sterilization of tissue allografts: A review

Singh

2016

WJR

Self Cite

167

137

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Tissue substitutes are required in a number of clinical conditions for treatment of injured and diseased tissues. Tissues like bone, skin, amniotic membrane and soft tissues obtained from human donor can be used for repair or reconstruction of the injured part of the body. Allograft tissues from human donor provide an excellent alternative to autografts. However, major concern with the use of allografts is the risk of infectious disease transmission. Therefore, tissue allografts should be sterilized to make them safe for clinical use. Gamma radiation has several advantages and is the most suitable method for sterilization of biological tissues. This review summarizes the use of gamma irradiation technology as an effective method for sterilization of biological tissues and ensuring safety of tissue allografts.

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“…Os esporos de B. pumilus têm sido usados como indicadores biológicos para esterilização por irradiação com raios gama (Darmady et al, 1961;Parisi & Antoine, 1974;Parisi & Antoine, 1975;Singh et al, 2006;Trabulsi & Alterthum, 2008;Pinto et al, 2010).…”

Section: Cálculos Dosimétricosunclassified

“…O estudo da eficácia esterilizante da atividade radioativa dos radiofármacos foi descrita em poucos trabalhos (Sorensen et al, 1977;Allwood & McCarthy, 1980 (Sorensen et al, 1977 A proposta deste trabalho é utilizar geradores com atividades radioativas mais altas, até 74 GBq (2.000 mCi), pois nenhuma publicação tratou da ampla faixa de atividade radioativa, nem da abrangência da investigação proposta, além do uso de microrganismos comprovadamente resistentes à radiação, como por exemplo, esporos de B. pumilus (Singh et al, 2006;Pinto et al, 2010). Este trabalho tem fundamental importância para a área da saúde, especialmente para a medicina preventiva e diagnóstica, cujos milhares de procedimentos realizados em medicina nuclear, principalmente nas áreas de oncologia, cardiologia e neurologia, beneficiam a popu-lação brasileira.…”

Section: Introductionunclassified

Estudo da influência da atividade radioativa presente no gerador de 99Mo/99mTc na esterilidade do produto terminado

Fukumori¹

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Radiation Resistance of the Microflora Associated with Amniotic Membranes

Cited by 9 publications

References 8 publications

Effects of Gamma Irradiation on Bacterial Microflora Associated with Human Amniotic Membrane

Effects of Gamma Irradiation on Bacterial Microflora Associated with Human Amniotic Membrane

Radiation sterilization of tissue allografts: A review

Estudo da influência da atividade radioativa presente no gerador de 99Mo/99mTc na esterilidade do produto terminado

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