Radiation-Induced Plasmid Dna Damage: Effect of Concentration and Length

Brabcová, Kateřina Pachnerová; Jamborová, Zuzana; Michaelidesová, Anna; Davídková, Marie; Kodaira, Satoshi; Šefl, Martin; Štěpán, Václav

doi:10.1093/rpd/ncz196

Cited by 4 publications

(11 citation statements)

References 11 publications

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“…Damage of plasmid DNA due to exposure to oxidizing and ionizing agents is shown to be a good approximation for cell DNA damage before preparation processes start. ,, The effect of an argon plasma jet coupled with aerosol and VUV/UV radiation from plasma was examined on plasmid DNA. Normally, commercial plasmid DNA (>90%) exists in supercoiled (SC) form.…”

Section: Resultsmentioning

confidence: 99%

Plasma Damage Control: From Biomolecules to Cells and Skin

Sremački

Kos

Bošnjak

et al. 2021

ACS Appl. Mater. Interfaces

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The antibacterial and cell-proliferative character of atmospheric pressure plasma jets (APPJs) helps in the healing process of chronic wounds. However, control of the plasma-biological target interface remains an open issue. High vacuum ultraviolet/ultraviolet (VUV/UV) radiation and RONS flux from plasma may cause damage of a treated tissue; therefore, controlled interaction is essential. VUV/UV emission from argon APPJs and radiation control with aerosol injection in plasma effluent is the focus of this research. The aerosol effect on radiation is studied by a fluorescent target capable of resolving the plasma oxidation footprint. In addition, DNA damage is evaluated by plasmid DNA radiation assay and cell proliferation assay to assess safety aspects of the plasma jet, the effect of VUV/UV radiation, and its control with aerosol injection. Inevitable emission of VUV/UV radiation from plasmas during treatment is demonstrated in this work. Plasma has no antiproliferative effect on fibroblasts in short treatments (t < 60 s), while long exposure has a cytotoxic effect, resulting in decreased cell survival. Radiation has no effect on cell survival in the medium due to absorption. However, a strong cytotoxic effect on the attached fibroblasts without the medium is apparent. VUV/UV radiation contributes 70% of the integral plasma effect in induction of single- and double-strand DNA breaks and cytotoxicity of the attached cells without the medium. Survival of the attached cells increases by 10% when aerosol is introduced between plasma and the cells. Injection of aerosol in the plasma effluent can help to control the plasma–cell/tissue interaction. Aerosol droplets in the effluent partially absorb UV emission from the plasma, limiting photon flux in the direction of the biological target. Herein, cold and safe plasma-aerosol treatment and a safe operational mode of treatment are demonstrated in a murine model.

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Section: Resultsmentioning

confidence: 99%

Plasma Damage Control: From Biomolecules to Cells and Skin

Sremački

Kos

Bošnjak

et al. 2021

ACS Appl. Mater. Interfaces

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“…Using different concentration of plasmids, no significant difference in DNA damage was observed by Brabcová et al. 21 Kong et al. 56 indicates that the proportion between free radical damage and direct ionizing damage is independent of DNA concentration when the DNA concentration is under a certain value (50 ng/μL).…”

Section: The Dna Plasmid As a Biodosimeter For Nano‐radiosensitizatio...mentioning

confidence: 92%

“…Studies showed that, the number of SSBs per DNA molecule is linearly proportional to plasmid size. 54 , 57 According to Brabcová et al., 21 radiation‐induced plasmid DNA damage within plasmids is influenced by plasmid length. Taken together and based on their results, they confirmed that longer plasmid molecules are more radiosensitive.…”

Section: The Dna Plasmid As a Biodosimeter For Nano‐radiosensitizatio...mentioning

confidence: 99%

“… 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 They have the advantage of being small DNA molecules, providing precise control over the scavenging environment and irradiation settings, as well as being easy to quantify by electrophoresis. 21 Furthermore, DNA plasmids are convenient for unraveling the physical/physicochemical radiosensitization mechanisms of nanoparticles (NPs) and quantifying the biological damages of radiation. This review aims at shedding light on various aspects of using different DNA plasmids as a molecular biodosimeter in nano‐radiosensitization studies.…”

Section: Introductionmentioning

confidence: 99%

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Nanoscopic biodosimetry using plasmid DNA in radiotherapy with metallic nanoparticles

Mansouri

Mesbahi

Hejazi

et al. 2022

J Applied Clin Med Phys

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Nanoscopic lesions (complex damages), are the most lethal lesions for the cells. As nanoparticles have become increasingly popular in radiation therapy and the importance of analyzing nanoscopic dose enhancement has increased, a reliable tool for nanodosimetry has become indispensable. In this regard, the DNA plasmid is a widely used tool as a nanodosimetry probe in radiobiology and nano‐radiosensitization studies. This approach is helpful for unraveling the radiosensitization role of nanoparticles in terms of physical and physicochemical effects and for quantifying radiation‐induced biological damage. This review discusses the potential of using plasmid DNA assays for assessing the relative effects of nano‐radiosensitizers, which can provide a theoretical basis for the development of nanoscopic biodosimetry and nanoparticle‐based radiotherapy.

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“…[1] Plasmid DNA is commonly used as a simpler substitute for a cell in studies of early effects of ionizing radiation, because it allows to determine yields of primary DNA lesions. [2] Numerous methods for enhancing the stability of recombinant organisms have been discovered. [3] Stable host-vector connections can be attained by adjusting the genetic makeup of recombinants as well as the environment under which the organisms are cultured.…”

Section: Introductionmentioning

confidence: 99%

Untitled

2022

AJP

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Objective:The purpose of the study delineates the growth and plasmid stability of DE3 host system. Methods: Different concentrations of drugs, chemicals, and various frequencies of radiations were subjected to the host system to verify the colony forming units, along with plasmid concentration and stability. Results: Among chemicals, acridine orange showed highest effect on growth of DH5a, while among the drugs, danthrone showed maximum effect on the growth of the organism. Radio frequency of 2GHz and low-intensity microwave radiation were recorded as highest inhibitory effects. However, there is no significant effect in growth that was observed in exposure to UV rays. Conclusion: The present work discussed that, the effect of drugs, chemicals, radio frequency, and microwave radiation has a huge effect not only on growth of organism but also concentration and stability of plasmid.

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Radiation-Induced Plasmid Dna Damage: Effect of Concentration and Length

Cited by 4 publications

References 11 publications

Plasma Damage Control: From Biomolecules to Cells and Skin

Plasma Damage Control: From Biomolecules to Cells and Skin

Nanoscopic biodosimetry using plasmid DNA in radiotherapy with metallic nanoparticles

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