Rabies Virus Neutralizing Antibody Profile in Cattle Vaccinated With Inactivated Vaccine Adjuvanted With Either Aluminum Hydroxide Alone or Combined With Avridine

Abstract

A study was conducted in Brazil from November 1999 to November 2000 to evaluate the antibody response of cattle vaccinated with an inactivated rabies virus vaccine adjuvanted with aluminum hydroxide (Al(OH)3) alone or combined with Avridine. Sixteen (16) female Holstein calves, 7 to 8 month old, with no history of vaccination against rabies were vaccinated (IM 2 mL) with the Al(OH)3 adjuvanted vaccine on days 0, 90 and 360, and, 16 similar calves were vaccinated (IM 2 mL) with the Avridine containing vaccine. On days 0, 30, 90, 120, 180, 360 and 390 post primary vaccination, blood samples were collected from all calves for antibody titration by serum neutralization test in mice, with fixed virus/varying serum method, using 100 LD50/0.03 mL of the CVS rabies virus strain. The two vaccines provoked antibody response with titers that persisted above 2.264 Log10 SN50 throughout the study. The mean antibody titer at 30 days p.v. was numerically higher for the Al(OH)3 adjuvanted vaccine, but the Avridine containing vaccine was numerically higher at day 90 although not significant. Following revaccination at day 90, the antibody level induced by the Avridine containing vaccine was higher and persisted higher until the end of the study. The difference was significant (p<0.05) on days 180, 360 and 390. Although the Avridine containing vaccine had a tendency to persist in higher level, both vaccines induced antibody titers that persisted >2.264 Log10 SN50 during the entire study period post initial vaccination.