Rabbit Cells Susceptible to Rubella Virus

Beale, A. J.; Christofinis, G; Furminger, I. G. S.

doi:10.1016/s0140-6736(63)90434-3

Cited by 71 publications

(23 citation statements)

References 3 publications

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“…14 The likelihood that renal tubule cell apoptosis is induced by bacterial cell wall components is supported by the acute changes in rabbit renal function induced by intravenous injection of muramyl dipeptide (MDP) and endotoxin. [15][16][17] Moreover, MDP (the smallest biologically active component of gram-positive and gram-negative bacteria cell wall peptidoglycan), peptidoglycan (PGN), and lipopolysaccharide (LPS) induce apoptosis in γ-glutamyl transpeptidase (GGT)-positive rabbit RK 13 renal epithelial cells, 18 as well as renal tubule cell lines of other mammalian species. 17 MDP and PGN induce rabbit kidney cell apoptosis via Ca 2+ -dependent interactions with surface calreticulin (CRT) 19 followed by FADD stimulation and effector caspase-3 activity 20 (CRT is a multi-functional Ca 2+ -binding chaperone protein in the ER).…”

Section: Introductionmentioning

confidence: 99%

Multiple Caspases Mediate Acute Renal Cell Apoptosis Induced by Bacterial Cell Wall Components

Langford

McGee

et al. 2011

Renal Failure

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The stimulus for caspase-mediated renal cell apoptosis in septic acute renal failure (ARF) is unclear. To demonstrate the nephrotoxic effects of bacterial cell wall components, the anti-cellular activity of bacterial muropeptides (muramyl dipeptides), peptidoglycans, and lipopolysaccharides was investigated in rabbit kidney cells. Changes in the cell membrane (APOPercentage™ dye uptake), caspase activities, and DNA degradation were quantified colorimetrically and using densitometric assays and their inhibition by caspase-specific and pan-caspase inhibitors was determined. The onset and levels of APOPercentage™ dye-positive rabbit kidney cells, caspase activities, and DNA degradation were closely associated. Specific caspase-1, -2, -3, -4, -8, -10, and -12 inhibitors reduced caspase-3 activity by ≥40%, but only caspase-3 and -8-specific inhibitors reduced apoptotic DNA levels. Pan-caspase inhibitor Q-VD-OPh was 10-fold more effective at inhibiting rabbit kidney cell death, caspase activation, and DNA degradation than caspase-family inhibitor Z-VAD-FMK. Apoptosis was inhibited effectively by both pan-caspase inhibitors when applied early during the stimulus-to-response period. Multiple initiator and effector caspases were activated suggesting extrinsic, intrinsic, and endoplasmic reticulum/stress apoptotic pathway stimulation in rabbit kidney cells treated with bacterial cell wall components. The results provide in vitro support for bacterial cell wall-induced apoptosis as a pathogenic mechanism of renal cell death in septic ARF and support the potential prophylactic use of pan-caspase inhibitors to suppress septic ARF.

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Section: Introductionmentioning

confidence: 99%

Multiple Caspases Mediate Acute Renal Cell Apoptosis Induced by Bacterial Cell Wall Components

Langford

McGee

et al. 2011

Renal Failure

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“…In the different laboratories the R.K.13 continuous line of rabbit kidney cells (Beale et al, 1963) Table I. These show good correlation, the methods adopted in the various laboratories being sufficiently uniform.…”

Section: Laboratory Methodsmentioning

confidence: 84%

Studies on Rubella in Pregnancy: Report of the Public Health Laboratory Service Working Party on Rubella

1968

BMJ

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Summary: In a further survey of women of childbearing age neutralizing antibody to rubella virus was found in 80 to 96% of those sampled from different parts of the country.When assessment was restricted to susceptible pregnant contacts and rubella in the index case was confirmed by virus isolation immunoglobulin in the dosage used did not appear to give any appreciable protection.One instance of a confirmed second attack after known exposure to rubella among more than 100 immune contacts is recorded.The implications of all these findings are discussed.

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“…Cell lines.-Mouse L cells (Sanford et al, 1948) and the RK13 line of rabbit kidney cells (Beale et al, 1963) were purchased from Gibco Bio-Cult, Glasgow. The A9 derivative of L cells (Littlefield, 1964) (Watkins, 1977).…”

Section: Methodsmentioning

confidence: 99%

Tumour-necrosis factor from the rabbit. I. Mode of action, specificity and physicochemical properties

Matthews¹,

Watkins²

1978

Br J Cancer

150

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Summary.-Sera from rabbits injected with BCG and then with endotoxin contain a factor (tumour-necrosis factor TNF) which, even at high dilutions, is cytotoxic in vitro for mouse L cells and some other cell lines. Using a 51Cr-release assay, cytotoxicity was detected as early as 7-8 h after addition of TNF serum to L cells and cell death was evident microscopically by 24 h. TNF was cytotoxic at 37°C but not at 21°C or 4°C, and acted on both dividing and non-dividing cells. The antimetabolites sodium azide and dinitrophenol partially protected L cells from TNF, suggesting that actively metabolizing cells are the most sensitive. Treatment of L cells with trypsin did not delay cytotoxicity nor was cytotoxicity inhibited in the presence of various saccharide derivatives of cell-surface glycoproteins. Rabbit TNF was remarkably stable with a mol. wt. of 40-50,000. It was eluted with the more acidic serum proteins on ion-exchange chromatography, but precipitated in 50o-saturated ammonium sulphate. Sensitivity to TNF could not be correlated with tumourigenicity of several animal and human lines tested nor with the production of C-type viruses.

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Rabbit Cells Susceptible to Rubella Virus

Cited by 71 publications

References 3 publications

Multiple Caspases Mediate Acute Renal Cell Apoptosis Induced by Bacterial Cell Wall Components

Multiple Caspases Mediate Acute Renal Cell Apoptosis Induced by Bacterial Cell Wall Components

Studies on Rubella in Pregnancy: Report of the Public Health Laboratory Service Working Party on Rubella

Tumour-necrosis factor from the rabbit. I. Mode of action, specificity and physicochemical properties

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