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Rabbani, Malik Ashiq; Iwabuchi, Aki; Murakami, Yukito; Suzuki, Tohru; Takayanagi, Kenji

doi:10.1023/a:1018305201279

Cited by 45 publications

(9 citation statements)

References 12 publications

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“…These results imply that multiplicity of factors including the geographical locations were responsible for the selection of genotypes that got naturalized at the sites of collections. Similar results have been reported earlier for several plant species (Gupta et al 1991; Dias et al 1993; Amurrio et al 1995; Rabbani et al 1998). It was interesting to note that accessions BM-11 and BM-14 from relatively nearer geographical origins with distinct phenotypes from other accessions (Table 1) formed a distinct cluster on the basis of dendrogram (Figs.…”

Section: Resultssupporting

confidence: 90%

Assessment of genetic variations among highly endangered medicinal plant Bacopa monnieri (L.) from Central India using RAPD and ISSR analysis

et al. 2012

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Genetic variations of 15 Brahmi (Bacopa monnieri L.) accessions were evaluated using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers. During RAPD analysis, amplification of genomic DNA of the 15 accessions by 22 primers generated 197 fragments, of which 187 were polymorphic with an average of 8.95 bands per primer. The amplified products varied in size from 2,200 to 250 bp. Twenty-five selected ISSR primers produced 284 bands across 15 accessions, of which 270 were polymorphic with an average of 10.80 bands per primer. The PIC value ranges from 0.363 to 0.908 for RAPD primers, while 0.419 to 0.836 in case of ISSR. The size of amplified bands ranged from 2,800 to 240 bp. Similarity index values ranged from 0.16 to 0.95 (RAPD), 0.18 to 0.98 (ISSR) and 0.179 to 0.945 for pooled ISSR and RAPD markers data. Mantel test revealed the similar distribution pattern of the polymorphism between RAPD and ISSR markers and the correlation co-efficient (r) was 0.71384. The results indicated that both of the marker systems RAPD and ISSR, individually or combined can be effectively used in determination of genetic relationship among B. Monnieri accessions collected from different parts of Central India. It could be concluded that the information of genetic similarities and diversity among Brahmi accessions is necessary for their conservation and breeding programs.

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Section: Resultssupporting

confidence: 90%

Assessment of genetic variations among highly endangered medicinal plant Bacopa monnieri (L.) from Central India using RAPD and ISSR analysis

et al. 2012

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“…These analysis techniques were mentioned in former studies to have a successful execution in mustard cultivars. 32 − 35 These techniques were also stated before in a lot of studies to be found efficiently in white head cabbage and can play a crucial role in the investigation of genetic diversity among diverse genotypes. 36 The distribution of studied B. napus L. cultivars into clusters was not due to their local or global origin and divergent habitat; rather, it was their variations at agro-morphological levels.…”

Section: Discussionmentioning

confidence: 85%

“…In the past, many plant breeders and scientists have applied and obtained promising variations for different Brassica cultivars in agro-morphological parameters with the help of two main complementary techniques of analysis, principal component analysis and cluster analysis. These analysis techniques were mentioned in former studies to have a successful execution in mustard cultivars. − These techniques were also stated before in a lot of studies to be found efficiently in white head cabbage and can play a crucial role in the investigation of genetic diversity among diverse genotypes . The distribution of studied B.…”

Section: Discussionmentioning

confidence: 96%

Morphological, Biochemical, and Molecular Characterization of Exotic Brassica Germplasm

Ali,

Bibi

et al. 2023

ACS Omega

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Oilseed rape (Brassica napus L.) is an important oilseed crop. We examined the diversity of germplasm expressed at three distinct levels (i.e., morphological, biochemical, and DNA levels). In this study, 150 B. napus L. accessions with three check varieties were provided by Bioresources Conservation Institute. The germplasm was grown in field conditions for data collection of 15 quantitative and nine qualitative agromorphological traits. The result indicated that for 15 quantitative agro-morphological traits, the highest coefficient of variation was recorded for plant height and days to flowering initiation. For nine qualitative traits, most of the accessions have a spatulate leaf, brown color seeds, yellow flowers, and erect silique attitude. The best adoptable genetically diverse exotic Brassica germplasms were selected, i.e., accessions 24178, 24881, 24199, 24214, 24242, and 24192. Based on biochemical analysis for high oil content and high oleic acid content, chakwal sarsoon and accession 24192 were selected. For high oleic and linoleic acids, accession 24181 performed best, for low erucic acid accessions 24177 and 24195. Based on molecular (SSR) markers, the top 50 selected genotypes were evaluated with 30 SSR markers. The 47 genotypes with three check varieties were clustered in six major groups; the coefficient of similarity ranged between 0.18 and 1.00. Based on SSR data, the germplasms accession 24178 and Abasin were the most diverse genotypes. These genotypes have the capacity and could be used in future breeding programs. High genetic variations were investigated through the SSR among the studied genotypes of Brassica napus L. The present study also concluded that SSR is a better technique for intraspecific genetic diversity. Other modern techniques should be applied such as SNIP for the investigation of a high level of genetic diversity among crop plants in the future.

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“…Westman and Stephen [1999] assessed the genetic diversity of Brassica germplasm collected from four different areas and their results showed that specific difference was observed among the genotypes belonging to similar region while in this there was no grouping observed for the geographical dispersal of the genotypes. This showed that a genetic base of Pakistani oilseed crops is narrow as suggested by Rabbani et al [1998].…”

Section: Discussionmentioning

confidence: 89%

Genetic diversity of Brassica rapa germplasm of Azad Jammu and Kashmir, Pakistan revealed by molecular markers

Khan¹,

Ashraf²,

Abbas³

et al. 2022

asphc

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Brassica species commonly known as rapeseed are the second largest oil yielding crop of Pakistan and belongs to the family Brassicaceae. This scientific investigation was carried out to explore the genetic diversity of Brassica rapa from Azad Jammu and Kashmir (AJK) Pakistan. Seeds of Brassica rapa L. genotypes of Azad Jammu and Kashmir were collected from Plant Genetic Research Institute (PGRI), National Agricultural Research Centre (NARC), Islamabad Pakistan. CTAB method was used for the extraction of DNA from leaf samples. SSR and ISSR markers were used to explore the genetic diversity and cluster analysis was used for data analysis. Out of 6 SSR markers, Ra2E11 and Na10-E02 showed maximum genetic diversity while Ra2-E07, Ra2-D04, and Na10-D03 showed moderate diversity while Ra2-E01 showed minimum genetic diversity. Among the elected genotypes 026503 (Bhimber), 026514 (Kotli), 026510 (Kotli) and 026539 (Bagh) showed maximum diversity with 6 SSR markers. From 10 ISSR markers ISSR 888 and ISSR 5 showed highest number of bands (7). ISSR 3, ISSR 5, ISSR 888 showed maximum genetic diversity among mustard genotypes, while ISSR 887 and ISSR 840 showed no results and ISSR 2, ISSR 4, ISSR 80, ISSR 811, ISSR 889 showed moderate diversity. Among the selected genotypes 026557 showed maximum diversity with ISSR markers. Based upon the present studies the genotypes 026503, 026514, 026510, 026539 and 026557 had been recognised as probable most significant genotypes which could be used in future plant breeding program.

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Cited by 45 publications

References 12 publications

Assessment of genetic variations among highly endangered medicinal plant Bacopa monnieri (L.) from Central India using RAPD and ISSR analysis

Assessment of genetic variations among highly endangered medicinal plant Bacopa monnieri (L.) from Central India using RAPD and ISSR analysis

Morphological, Biochemical, and Molecular Characterization of Exotic Brassica Germplasm

Genetic diversity of Brassica rapa germplasm of Azad Jammu and Kashmir, Pakistan revealed by molecular markers

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