R2R3‐<scp>MYB</scp> transcription factor <scp>MYB</scp>6 promotes anthocyanin and proanthocyanidin biosynthesis but inhibits secondary cell wall formation in <i>Populus tomentosa</i>

Wang, Lijun; Lu, Wei; Ran, Lingyu; Dou, Liwen; Yao, Shixiang; Hu, Jian; Fan, Di; Li, Chaofeng; Luo, Keming

doi:10.1111/tpj.14364

Cited by 159 publications

(100 citation statements)

References 93 publications

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“…It has been demonstrated that the phenylpropanoid and flavonoid biosynthesis pathways are regulated by different TF classes, such as MYB, WRKY, NAC, and bHLH, through modulating the expression of the related structural genes. Among these TFs, MYB-bHLH-WD40 repeat (MBW) ternary complexes have been illustrated to be one of the most important regulatory components that are involved in phenylpropanoid and flavonoid biosynthesis [52][53][54][55][56]. To date, certain MBW component genes that regulate flavonoid biosynthesis have been identified in tea plants.…”

Section: Discussionmentioning

confidence: 99%

Integrative Transcriptomic and Metabolic Analyses Provide Insights into the Role of Trichomes in Tea Plant (Camellia Sinensis)

Cao

et al. 2020

Biomolecules

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Trichomes, which develop from epidermal cells, are regarded as one of the key features that are involved in the evaluation of tea quality and tea germplasm resources. The metabolites from trichomes have been well characterized in tea products. However, little is known regarding the metabolites in fresh tea trichomes and the molecular differences in trichomes and tea leaves per se. In this study, we developed a method to collect trichomes from tea plant tender shoots, and their main secondary metabolites, including catechins, caffeine, amino acids, and aroma compounds, were determined. We found that the majority of these compounds were significantly less abundant in trichomes than in tea leaves. RNA-Seq was used to investigate the differences in the molecular regulatory mechanism between trichomes and leaves to gain further insight into the differences in trichomes and tea leaves. In total, 52.96 Gb of clean data were generated, and 6560 differentially expressed genes (DEGs), including 4471 upregulated and 2089 downregulated genes, were identified in the trichomes vs. leaves comparison. Notably, the structural genes of the major metabolite biosynthesis pathways, transcription factors, and other key DEGs were identified and comparatively analyzed between trichomes and leaves, while trichome-specific genes were also identified. Our results provide new insights into the differences between tea trichomes and leaves at the metabolic and transcriptomic levels, and open up new doors to further recognize and re-evaluate the role of trichomes in tea quality formation and tea plant growth and development.

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Section: Discussionmentioning

confidence: 99%

Integrative Transcriptomic and Metabolic Analyses Provide Insights into the Role of Trichomes in Tea Plant (Camellia Sinensis)

Cao

et al. 2020

Biomolecules

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“…The MYB TF (EUC13670) and bHLH (EUC04483) were supposed to regulated anthocyanin synthesis in H12 leaves. Wang reported R2R3-MYB TF MYB6 of Populus tomentosa was mainly expressed in young leaves and the overexpression of MYB6 in transgenic poplar resulted in the signi cant up-regulation of anthocyanin and procyanidin accumulation [32]. In addition, PpGST together with PpMYB10.1 might regulate the anthocyanin accumulation [33].…”

Section: Structural Genes and Transcription Factors Involved In Anthomentioning

confidence: 99%

Metabolome and Transcriptome Analyses of Red-colored Leaves From Eucommia Ulmoides ‘Huazhong No. 12’

Yang¹,

Chen²,

Ding³

et al. 2020

Preprint

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Background: Eucommia ulmoides ‘Oliver’ is an economically important tree species with highly medicinal and ecological values that is naturally distributed in China. E. ulmoides ‘Huazhong No. 12’ (H12) is the only red-leaf genotype of this species. In this study, the pigment contents of H12 and E. ulmoides ‘Huazhong No. 11’ (H11, green leaves) were determined. The differential metabolites in H12 and H11 were detected by UPLC-MS/MS, and the differentially expressed genes were screened by transcriptome. Then the key metabolites and corresponding gene regulation in anthocyanin related metabolic pathway were analyzed.Results: The chlorophyll a, chlorophyll b and carotenoid contents in H12 leaves were lower than H11, while the total anthocyanin content in H12 was 4.06 times higher than in H11. There were 96 up-regulated metabolites in H12, including anthocyanin, proanthocyanidins, flavonoid and flavonol. Among them, four differentially expressed anthocyanins were identified. A total of 8,368 differentially expressed genes were selected from transcriptome between H12 and H11. The flavone and flavonol biosynthesis pathway, anthocyanin pathway and photosynthetic pathway were analyzed. Finally, EuCHI, EuF3'H, EuF3'5'H, EuDFR and Eu3MaT1 were recommended as the key genes. The cyanidin, cyanidin 3-malonyl-glucoside and cyanidin 3, 5-glucoside were responsible for the H12 red leaves.Conclusion: This study revealed the metabolites and gene regulation of anthocyanin synthesis, and the potential function between the anthocyanin and photosynthetic gene expression in E. ulmoides red leaves. Notably, the results also provided a reference for the study of other plant leaf-coloring mechanisms.

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“…TFs are also powerful targets for manipulating secondary metabolic pathways in plants (Cao et al 2018;Huang et al 2019;Wang et al 2019aWang et al , 2019b. However, little is known about the regulatory mechanisms or target genes of TFs involved in the phenolic acid biosynthesis pathway in S. miltiorrhiza.…”

Section: Introductionmentioning

confidence: 99%

“…MYB proteins comprise a huge family of TFs that play important roles in plant development and secondary metabolism (Borevitz et al 2000;Czemmel et al 2012;Chen et al 2019;Hao et al 2020). MYB TFs are classified into four subfamilies based on the number of conserved MYB domains in their N-terminal regions, 1R-MYB, R2R3-MYB, R1R2R3-MYB, and 4R-MYB TFs, with R2R3-MYBs representing the major subfamily in plants (Rosinski and Atchley 1998;Dubos et al 2010;An et al 2015;Wang et al 2019aWang et al , 2019bHao et al 2020). For example, NtMYB3 represses flavonol biosynthesis in Narcissus tazetta (Anwar et al 2019).…”

Section: Introductionmentioning

confidence: 99%

“…For example, NtMYB3 represses flavonol biosynthesis in Narcissus tazetta (Anwar et al 2019). The R2R3-MYB TF MYB6 promotes anthocyanin and proanthocyanidin biosynthesis in Populus tomentosa (Wang et al 2019a(Wang et al , 2019b. Overexpressing R2R3-MYB TF SmMYB9b (Zhang et al 2017) or SmMYB98 (Hao et al 2020) enhanced tanshinone concentrations in S. miltiorrhiza.…”

Section: Introductionmentioning

confidence: 99%

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SmMYB2 promotes salvianolic acid biosynthesis in the medicinal herb Salvia miltiorrhiza

Deng

Wang

Huang

et al. 2020

JIPB

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MYB transcription factors play vital roles in plant growth and metabolism. The phytohormone methyl jasmonate (MeJA) promotes phenolic acid accumulation in the medicinal herb Salvia miltiorrhiza, but the regulatory mechanism is poorly understood. Here, we identified the MeJA-responsive R2R3-MYB transcription factor gene SmMYB2 from a transcriptome library produced from MeJAtreated S. miltiorrhiza hairy roots. SmMYB2 expression was tightly correlated with the expression of key salvianolic acid biosynthetic genes including CYP98A14. SmMYB2 was highly expressed in the periderm of S. miltiorrhiza and SmMYB2 localized to the nucleus. Overexpressing SmMYB2 in S. miltiorrhiza hairy roots significantly increased the levels of salvianolic acids (including rosmarinic acid and salvianolic acid B) by upregulating salvianolic acid biosynthetic genes such as CYP98A14. SmMYB2 binds to the MYB-binding motifs in the promoter of CYP98A14, as confirmed by a dual-luciferase assay and electrophoretic mobility shift assays. Anthocyanin contents were significantly higher in SmMYB2-overexpressing hairy root lines than the control, primarily due to the increased expression of CHI, DFR, and ANS. These findings reveal the novel regulatory role of SmMYB2 in MeJA-mediated phenolic acid biosynthesis, providing a useful target gene for metabolic engineering and shedding light on the salvianolic acid regulatory network.

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R2R3‐MYB transcription factor MYB6 promotes anthocyanin and proanthocyanidin biosynthesis but inhibits secondary cell wall formation in Populus tomentosa

Cited by 159 publications

References 93 publications

Integrative Transcriptomic and Metabolic Analyses Provide Insights into the Role of Trichomes in Tea Plant (Camellia Sinensis)

Integrative Transcriptomic and Metabolic Analyses Provide Insights into the Role of Trichomes in Tea Plant (Camellia Sinensis)

Metabolome and Transcriptome Analyses of Red-colored Leaves From Eucommia Ulmoides ‘Huazhong No. 12’

SmMYB2 promotes salvianolic acid biosynthesis in the medicinal herb Salvia miltiorrhiza

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