Quercetin Increases Oxidative Stress Resistance and Longevity in <i>Saccharomyces cerevisiae</i>

Belinha, Iracema; Amorim, Maria Amélia; Rodrigues, Pedro; Freitas, Víctor de; Moradas‐Ferreira, Pedro; Mateus, Nuno; Costa, Vítor Santos

doi:10.1021/jf063302e

Cited by 122 publications

(91 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Quantification of carbonyls was performed by densitometry. Lipid peroxidation was determined by quantifying thiobarbituric acid reactive substances, as described (Belinha et al, 2007) and expressed as nmol MDA (mg protein) Ϫ1 . The oxidant-sensitive probe 2Ј,7Ј-dichlorodihydrofluorescein diacetate (H 2 DCFDA) was used to measure the levels of intracellular oxidation (Davidson et al, 1996).…”

Section: Protein Carbonylation Lipid Peroxidation and Intracellular mentioning

confidence: 99%

See 1 more Smart Citation

Isc1p Plays a Key Role in Hydrogen Peroxide Resistance and Chronological Lifespan through Modulation of Iron Levels and Apoptosis

Almeida

Marques

Mojžita

et al. 2008

MBoC

Self Cite

107

View full text Add to dashboard Cite

The inositolphosphosphingolipid phospholipase C (Isc1p) of Saccharomyces cerevisiae belongs to the family of neutral sphingomyelinases that generates the bioactive sphingolipid ceramide. In this work the role of Isc1p in oxidative stress resistance and chronological lifespan was investigated. Loss of Isc1p resulted in a higher sensitivity to hydrogen peroxide that was associated with an increase in oxidative stress markers, namely intracellular oxidation, protein carbonylation, and lipid peroxidation. Microarray analysis showed that Isc1p deficiency up-regulated the iron regulon leading to increased levels of iron, which is known to catalyze the production of the highly reactive hydroxyl radicals via the Fenton reaction. In agreement, iron chelation suppressed hydrogen peroxide sensitivity of isc1⌬ mutants. Cells lacking Isc1p also displayed a shortened chronological lifespan associated with oxidative stress markers and aging of parental cells was correlated with a decrease in Isc1p activity. The analysis of DNA fragmentation and caspase-like activity showed that Isc1p deficiency increased apoptotic cell death associated with oxidative stress and aging. Furthermore, deletion of Yca1p metacaspase suppressed the oxidative stress sensitivity and premature aging phenotypes of isc1⌬ mutants. These results indicate that Isc1p plays an important role in the regulation of cellular redox homeostasis, through modulation of iron levels, and of apoptosis.

show abstract

Section: Protein Carbonylation Lipid Peroxidation and Intracellular mentioning

confidence: 99%

“…Yeast extracts were prepared as described (Belinha et al, 2007), and glutathione was assayed by the method of Tietze (1969). The rate of color development was monitored at 405 nm.…”

Section: Glutathione Determinationmentioning

confidence: 99%

Isc1p Plays a Key Role in Hydrogen Peroxide Resistance and Chronological Lifespan through Modulation of Iron Levels and Apoptosis

Almeida

Marques

Mojžita

et al. 2008

MBoC

Self Cite

107

View full text Add to dashboard Cite

show abstract

“…Yeasts can incorporate polyphenolic antioxidants into cells because these natural compounds exert several physiological effects on yeast cells, e.g., curcumin and quercetin show radioprotective action (Nemavarkar et al, 2004), and pretreatment with quercetin increases H 2 O 2 resistance and the chronological life span of yeast cells (Belinha et al, 2007). We measured antioxidant activity of quercetin in the hypertonic culture medium by a chemical assay, and observed that about 80% activity for eliminating superoxide still remained after 48 h cultivation.…”

Section: Sod1 Sod2mentioning

confidence: 99%

A Simple Growth Test of a Saccharomyces cerevisiae Cu, Zn-Superoxide Dismutase-Deficient Mutant in Hypertonic Medium for Biological Evaluation of Antioxidants

Tamura¹,

Wada²,

Hase³

et al. 2010

FSTR

View full text Add to dashboard Cite

Osmotic stress hampers the growth of Saccharomyces cerevisiae, and this effect is mediated by oxidative stress. A simple test for the biological evaluation of antioxidants was developed on the basis of recovery of growth delay of Cu, Zn-superoxide dismutase-lacking yeast (SOD1Δ) under hyperosmosis. The SOD1Δ strain suffered from growth inhibition in a medium containing 1.7 M sorbitol. Protein oxidation in SOD1Δ cells was greatly increased by exposure to a hypertonic medium, indicating the accumulation of reactive oxygen species followed by oxidation of cellular materials. The hyperosmosis-induced growth arrest of SOD1Δ was abolished by the addition of L-ascorbic acid to the medium; the antioxidant effect depended on the concentration, ranging from 1 to 10 mM. Cysteine, N-acetyl-cysteine, and glutathione were also able to restore the growth of SOD1Δ cells. High concentrations of these thiol compounds proved less effective, probably due to adverse effects of an excess of these antioxidants. No growth restoration was seen for typical polyphenol antioxidants, including curcumin, quercetin and catechin.

show abstract

“…We tested different methods of stimulating oxidative stress: by use of hydrogen peroxide (H 2 O 2 ) or menadione. Both menadione and H 2 O 2 can generate the hydroxyl radical (HO•), the most toxic reactive oxygen species (ROS) (Roginsky et al, 1996;Belinha et al, 2007). Menadione can also cause an oxidative stress by forming a complex with glutathione, the main antioxidant thiol (Steels et al, 1994).…”

Section: In Vivo and In Vitro Antioxidant Assay Of S Cayennensismentioning

confidence: 99%

Evaluation of antimicrobial, antioxidant and phototoxic activities of extracts and isolated compounds from Stachytarpheta cayennensis (Rich.) Vahl, Verbenaceae

Souza¹,

Silva²,

Machado³

et al. 2010

Rev. bras. farmacogn.

View full text Add to dashboard Cite

RESUMO: "Avalição da atividade antimicrobiana, antioxidante e fototóxica dos extratos e compostos isolados de Stachytarpheta cayennensis (Rich.) Vahl, Verbenaceae". Stachytarpheta cayennensis (Rich.) Vahl, Verbenaceae, é uma planta utilizada na medicina popular brasileira contra úlceras e lesões de pele. Internamente é usada contra inflamações, febre, doenças renais e aterosclerose. Essa planta foi estudada com o objetivo de identificar os compostos bioativos majoritários que possam justificar seu uso terapêutico contra lesões de pele e arteriosclerose. A atividade antioxidante do extrato bruto etanólico, partições e os compostos majoritários isolados das raízes de S. cayennesis foi avaliada através de testes in vivo e in vitro. In vitro a atividade antioxidante foi avaliada pelo teste fotocolorimétrico do radical 2,2-difenil-1-picrilidrazil (DPPH•). In vivo, Saccharomyces cerevisiae, um modelo de célula eucariótica, foi utilizado tanto para avaliar a fototoxicidade quanto a capacidade antioxidante contra as espécies reativas de oxigênio (EROS) menadiona e peróxido de hidrogênio. O extrato, partições e os dois compostos majoritários isolados, verbascosídeo e ácido betulínico foram capazes de aumentar a sobrevivência e diminuir a peroxidação lipídica de S. cerevisiae contra EROS. Verbascosídeo apresentou atividade antimicrobiana moderada contra Streptococcus pyogenes, S.epidermidis e Staphylococcus aureus. O extrato etanólico e as partições testadas não apresentaram fototoxicidade, indicando que S. cayennensis é uma planta segura para o tratamento de lesões de pele e como possível ingrediente em cosméticos.Unitermos: Stachytarpheta cayennensis, antioxidante, antimicrobial, fototoxicidade, arilpropanoide glicosilado, ácido betulínico.ABSTRACT: Stachytarpheta cayennensis (Rich.) Vahl, Verbenaceae, plant extract, is a Brazilian medicinal plant externally used in folk medicine for purulent ulcers, skin lesions and internally for inflammations, fever, renal disorders and atherosclerosis. S. cayennesis was studied to identify potential bioactive compounds that may justify their therapeutic use against skin lesions and atherosclerosis. The antioxidant, antimicrobial and phototoxicity capacities of the crude ethanolic extract, fractions and isolated compounds from roots of S. cayennesis were evaluated through in vivo and in vitro tests. Strains of Saccharomyces cerevisiae, an eukaryotic cell model, were used to assess both the phototoxicity and the capacity to protect against the lethal oxidative stress caused by menadione and hydrogen peroxide. The extract, fractions and the two major isolated compounds, verbascoside and betulinic acid, of S. cayennensis were able to increase the tolerance and decrease the lipid peroxidation of S. cerevisiae to reactive oxygen species (ROS). The antioxidant activity was also evaluated by scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH•). Verbascoside exhibited a moderate antimicrobial activity against Streptococcus pyogenes, S.epidermidis and Staphylococcus aureus. Neither the ethanol...

show abstract

Quercetin Increases Oxidative Stress Resistance and Longevity in Saccharomyces cerevisiae

Cited by 122 publications

References 38 publications

Isc1p Plays a Key Role in Hydrogen Peroxide Resistance and Chronological Lifespan through Modulation of Iron Levels and Apoptosis

Isc1p Plays a Key Role in Hydrogen Peroxide Resistance and Chronological Lifespan through Modulation of Iron Levels and Apoptosis

A Simple Growth Test of a Saccharomyces cerevisiae Cu, Zn-Superoxide Dismutase-Deficient Mutant in Hypertonic Medium for Biological Evaluation of Antioxidants

Evaluation of antimicrobial, antioxidant and phototoxic activities of extracts and isolated compounds from Stachytarpheta cayennensis (Rich.) Vahl, Verbenaceae

Contact Info

Product

Resources

About