Quercetin attenuates cell apoptosis of oxidant-stressed SK-N-MC cells while suppressing up-regulation of the defensive element, HIF-1α

Roshanzamir, Fariba; Yazdanparast, Razieh

doi:10.1016/j.neuroscience.2014.07.036

Cited by 23 publications

(21 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bcl-2 proteins family and caspase-3 proteins family are two main apoptosis proteins. Tang et al found that bone mesenchymal stem cells possess potent immunomodulatory and anti-oxidative stress properties (Roshanzamir, 2014). The Bcl-2 protein family is divided into two groups: inhibiting apoptosis group (Bcl-w, Bcl-10, Bcl-xl, and Bcl-2) and promoting apoptosis group (Bax, Bcl-rambo, and Bcl-xs) (Ethell and Fei, 2009).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bone mesenchymal stem cell‐conditioned medium attenuates the effect of oxidative stress injury on NSCs by inhibiting the Notch1 signaling pathway

Niu

Xiang²,

Song

et al. 2019

Cell Biology International

View full text Add to dashboard Cite

Numerous studies have demonstrated the therapeutic effect of bone mesenchymal stem cells on spinal cord injury (SCI), especially on neural stem cells (NSCs). However, the predominant mechanisms of bone mesenchymal stem cells (BMSCs) are unclear. Recently, some researchers have found that paracrine signaling plays a key role in the therapeutic capacity of BMSCs and emphasized that the protective effect of BMSCs may be due to paracrine factors. In this study, we aimed to investigate the potential mechanisms of BMSCs to protect NSCs. NSCs were identified by immunocytochemistry. The oxidative stress environment was simulated by H 2 O 2 (50, 100, 200 μM) for 2 h. The apoptotic rate of the NSCs was detected via flow cytometry. Lactate dehydrogenase (LDH), malondialdehyde (MDA), and superoxide dismutase (SOD) activity were evaluated via corresponding assay kits. Western blot was used to detect the expressions of Notch1, HES1, caspase-3, cleave caspase-3, Bax, and Bcl-2. We found that H 2 O 2 could significantly induce the apoptosis of NSCs, increase LDH, MDA levels, and decrease SOD activity by activating the Notch1 signaling pathway. DAPT (the specific blocker of Notch1) and BMSC-conditioned medium (BMSC-CM) could significantly prevent the apoptotic effect and oxidative stress injury on NSCs that were treated with H 2 O 2 . We also revealed that BMSC-CM could decrease the expression of Notch1, Hes1, cleave caspase-3, Bax, and increases the expression of Bcl-2 in NSCs, which was induced by H 2 O 2 . These results have revealed that BMSC-CM can neutralize the effect against oxidative stress injury on the apoptosis of NSCs by inhibiting the Notch1 signaling pathway.

show abstract

Section: Introductionmentioning

confidence: 99%

“…Numerous studies have demonstrated the therapeutic function of bone mesenchymal stem cells on SCI. Tang et al found that bone mesenchymal stem cells possess potent immunomodulatory and anti-oxidative stress properties (Roshanzamir, 2014). However, the predominant mechanisms of bone mesenchymal stem cells (BMSCs) are unclear.…”

Section: Introductionmentioning

confidence: 99%

Bone mesenchymal stem cell‐conditioned medium attenuates the effect of oxidative stress injury on NSCs by inhibiting the Notch1 signaling pathway

Niu

Xiang²,

Song

et al. 2019

Cell Biology International

View full text Add to dashboard Cite

show abstract

“…The relationship between GAPDH and p53, a transcription factor that regulates expression of stress response genes is also observed. Protein p53 is upregulated and promotes death in response to diverse genotoxic cellular stresses, including oxidative stress [10,41]. In glutamate-induced oxidative stress, GAPDH translocates to the nucleus, enhances p53 expression and activates p53-mediated death pathway [42].…”

Section: Quercetin Did Not Improve H 2 O 2 -Induced Decrease In Glutamentioning

confidence: 99%

“…Both in vitro and in vivo quercetin is effective against various oxidants and other neurotoxic molecules that induce oxidative stress and mimic the pathological hallmarks of neurodegenerative diseases [8,9]. In vitro, quercetin exerts its neuroprotective effects acting as a potent direct radical scavenger and a metal chelator but is also able to downregulate redox-sensitive signalling [10,11]. Due to low bioavailability, the expected concentrations of quercetin in the brain tissue are below those that are required for direct antioxidant activity.…”

Section: Introductionmentioning

confidence: 99%

Atomic force microscopy reveals new biomarkers for monitoring subcellular changes in oxidative injury: neuroprotective effects of quercetin at the nanoscale

Jembrek

Vlaini

ade

et al. 2018

Preprint

View full text Add to dashboard Cite

Oxidative stress is a process involved in the pathogenesis of many diseases, including atherosclerosis, hypertension, diabetes, Alzheimer's disease etc. The biomarkers for assessing the degree of oxidative stress have been attracting much interest because of their potential clinical relevance in understanding cellular effects of free radicals and evaluation of the efficacy of drug treatment. Here, an interdisciplinary approach using atomic force microscopy (AFM) and cellular and biological molecular methods were used to obtain new potential biomarkers for monitoring oxidative stress condition. Biological methods confirmed the oxidative damage of investigated P19 neurons and revealed the underlying mechanism of quercetin protective action. AFM was employed to evaluate morphological (roughness) and nanomechanical (elasticity) properties that may be specific biomarkers for oxidative stress-induced cytoskeletal reorganization manifested by changes in the lateral dimension and height of neuronal somas.The morphological and nanomechanical analysis of neurons showed the strong mutual correlation between changes in cell membrane elasticity and neuroprotective effects of quercetin. Our findings indicate that AFM is a highly valuable tool for biomedical applications, detection and clarifying of drug-induced changes at the nanoscale and emphasize the potential of AFM approach in the development of novel therapeutic strategies directed against oxidative stress-induced neurodegeneration.

show abstract

“…Similar to H63D cells, the protein carbonyl level in vector and WT HFE cells significantly decreased after treatment with okra. Since quercetin is a constituent of okra and has been reported to decrease oxidative stress in various models [15], we investigated the effect of this compound also (Fig. 1B).…”

Section: Effects Of Okra and Quercetin On Ros H 2 O 2 And Protein Oxmentioning

confidence: 99%

The effects of okra (Abelmoschus esculentus Linn.) on the cellular events associated with Alzheimer’s disease in a stably expressed HFE neuroblastoma SH-SY5Y cell line

Mairuae

Connor

Lee

et al. 2015

Neuroscience Letters

View full text Add to dashboard Cite

Quercetin attenuates cell apoptosis of oxidant-stressed SK-N-MC cells while suppressing up-regulation of the defensive element, HIF-1α

Cited by 23 publications

References 64 publications

Bone mesenchymal stem cell‐conditioned medium attenuates the effect of oxidative stress injury on NSCs by inhibiting the Notch1 signaling pathway

Bone mesenchymal stem cell‐conditioned medium attenuates the effect of oxidative stress injury on NSCs by inhibiting the Notch1 signaling pathway

Atomic force microscopy reveals new biomarkers for monitoring subcellular changes in oxidative injury: neuroprotective effects of quercetin at the nanoscale

The effects of okra (Abelmoschus esculentus Linn.) on the cellular events associated with Alzheimer’s disease in a stably expressed HFE neuroblastoma SH-SY5Y cell line

Contact Info

Product

Resources

About