Quantum Dot Encapsulation in Viral Capsids

Dixit, Suraj; Goicochea, Nancy L.; Daniel, Marie‐Christine; Murali, Ayaluru; Bronstein, Lyudmila M.; De, Mrinmoy; Stein, Barry D.; Rotello, Vincent M.; Kao, C. Cheng; Dragnea, Bogdan

doi:10.1021/nl061165u

Cited by 196 publications

(184 citation statements)

References 37 publications

(73 reference statements)

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“…Inorganic nanoparticles, such as semiconductor quantum dots, are also promising for use as probes 10,11 . Quantum dots are much brighter and more robust than fluorescent dyes and proteins, but their bulky size places severe limits on their application.…”

Section: Virus and Cell Labelingmentioning

confidence: 99%

Virus trafficking – learning from single-virus tracking

2007

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What could be a better way to study virus trafficking than 'miniaturizing oneself' and 'taking a ride with the virus particle' on its journey into the cell? Single-virus tracking in living cells potentially provides us with the means to visualize the virus journey. This approach allows us to follow the fate of individual virus particles and monitor dynamic interactions between viruses and cellular structures, revealing previously unobservable infection steps. The entry, trafficking and egress mechanisms of various animal viruses have been elucidated using this method. The combination of single-virus trafficking with systems approaches and state-of-the-art imaging technologies should prove exciting in the future.

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Section: Virus and Cell Labelingmentioning

confidence: 99%

Virus trafficking – learning from single-virus tracking

2007

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“…Our models are motivated by recent experiments in which Brome mosaic virus (BMV) capsid proteins dynamically encapsidate functionalized inorganic nanoparticle cores, creating unique biological and synthetic composite structures called virus-like particles (VLPs) [33][34][35][36]. By combining the unparalleled self-assembly and targeting capabilities of viruses with the functionalizability of nanoparticles, VLPs show promise as imaging agents [36][37][38][39], diagnostic and therapeutic vectors [40][41][42], and as subunits or templates for synthesis of advanced nanomaterials [43][44][45][46].…”

Section: Introductionmentioning

confidence: 99%

Controlling viral capsid assembly with templating

Hagan

2008

Phys. Rev. E

104

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We develop coarse-grained models that describe the dynamic encapsidation of functionalized nanoparticles by viral capsid proteins. We find that some forms of cooperative interactions between protein subunits and nanoparticles can dramatically enhance rates and robustness of assembly, as compared to the spontaneous assembly of subunits into empty capsids. For large core-subunit interactions, subunits adsorb onto core surfaces en masse in a disordered manner, and then undergo a cooperative rearrangement into an ordered capsid structure. These assembly pathways are unlike any identified for empty capsid formation. Our models can be directly applied to recent experiments in which viral capsid proteins assemble around the functionalized inorganic nanoparticles [Sun et al., Proc. Natl. Acad. Sci (2007) 104, 1354. In addition, we discuss broader implications for understanding the dynamic encapsidation of single-stranded genomic molecules during viral replication and for developing multicomponent nanostructured materials.

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“…Some applications of virus-like particles for delivery of solid nanoparticles, 22 imaging reagents, 23 and small molecule drugs 24 have already been reported, but they have mainly focused on particles derived from plant viruses or bacteriophages, eg, Cowpea mosaic virus, Cowpea chorotic mottle virus, and bacteriophage MS2. Very few such applications of virus-like particles have been derived from human viruses due to the fact that human viruses may cause immune responses.…”

Section: Discussionmentioning

confidence: 99%

A novel polyethyleneimine-coated adeno-associated virus-like particle formulation for efficient siRNA delivery in breast cancer therapy: preparation and in vitro analysis

Shao

Paul²,

Abbasi³

et al. 2012

IJN

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Background: Systemic delivery of small interfering RNA (siRNA) is limited by its poor stability and limited cell-penetrating properties. To overcome these limitations, we designed an efficient siRNA delivery system using polyethyleneimine-coated virus-like particles derived from adeno-associated virus type 2 (PEI-AAV2-VLPs). Methods: AAV2-VLPs were produced in insect cells by infection with a baculovirus vector containing three AAV2 capsid genes. Using this method, we generated well dispersed AAV2-VLPs with an average diameter of 20 nm, similar to that of the wild-type AAV2 capsid. The nanoparticles were subsequently purified by chromatography and three viral capsid proteins were confirmed by Western blot. The negatively charged AAV2-VLPs were surface-coated with PEI to develop cationic nanoparticles, and the formulation was used for efficient siRNA delivery under optimized transfection conditions. Results: PEI-AAV2-VLPs were able to condense siRNA and to protect it from degradation by nucleases, as confirmed by gel electrophoresis. siRNA delivery mediated by PEI-AAV2-VLPs resulted in a high transfection rate in MCF-7 breast cancer cells with no significant cytotoxicity. A cell death assay also confirmed the efficacy and functionality of this novel siRNA formulation towards MCF-7 cancer cells, in which more than 60% of cell death was induced within 72 hours of transfection. Conclusion: The present study explores the potential of virus-like particles as a new approach for gene delivery and confirms its potential for breast cancer therapy.

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Quantum Dot Encapsulation in Viral Capsids

Cited by 196 publications

References 37 publications

Virus trafficking – learning from single-virus tracking

Virus trafficking – learning from single-virus tracking

Controlling viral capsid assembly with templating

A novel polyethyleneimine-coated adeno-associated virus-like particle formulation for efficient siRNA delivery in breast cancer therapy: preparation and in vitro analysis

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