Quantitative study of tumor angiogenesis in three-dimensional matrigel barrier using electric impedance measurement technique

Huang, Chun‐Hao; Lei, Kin Fong

doi:10.1016/j.snb.2022.132458

Cited by 7 publications

(3 citation statements)

References 40 publications

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“…Furthermore, VEGF can be delivered indirectly by some types of cells such as macrophages, fibroblasts, keratinocytes, platelets, and tumor cells. One of the most often examined co-cultures consists of tumor spheroids and endothelial cells [ 43 , 44 , 45 , 46 , 47 , 48 , 49 ]. The pro-angiogenic activity of tumor spheroids can be enhanced by the addition of stromal cells such as fibroblasts [ 33 , 37 ].…”

Section: Discussionmentioning

confidence: 99%

Exploring Endothelial Expansion on a Chip

Konopka

Flont

Żuchowska

et al. 2022

Sensors

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Angiogenesis is the development of new blood vessels from the existing vasculature. Its malfunction leads to the development of cancers and cardiovascular diseases qualified by the WHO as a leading cause of death worldwide. A better understanding of mechanisms regulating physiological and pathological angiogenesis will potentially contribute to developing more effective treatments for those urgent issues. Therefore, the main goal of the following study was to design and manufacture an angiogenesis-on-a-chip microplatform, including cylindrical microvessels created by Viscous Finger Patterning (VFP) technique and seeded with HUVECs. While optimizing the VFP procedure, we have observed that lumen’s diameter decreases with a diminution of the droplet’s volume. The influence of Vascular Endothelial Growth Factor (VEGF) with a concentration of 5, 25, 50, and 100 ng/mL on the migration of HUVECs was assessed. VEGF’s solution with concentrations varying from 5 to 50 ng/mL reveals high angiogenic potential. The spatial arrangement of cells and their morphology were visualized by fluorescence and confocal microscopy. Migration of HUVECs toward loaded angiogenic stimuli has been initiated after overnight incubation. This research is the basis for developing more complex vascularized multi-organ-on-a-chip microsystems that could potentially be used for drug screening.

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Section: Discussionmentioning

confidence: 99%

Exploring Endothelial Expansion on a Chip

Konopka

Flont

Żuchowska

et al. 2022

Sensors

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“…74,75 Another prominent material used in angiogenesis-on-chip models is Matrigel. 76,77 Matrigel is a prominent hydrogel material that contains imperative structural proteins (laminin, collagen, heparan sulfate, etc.) that promote cell differentiation, proliferation, and angiogenesis.…”

Section: Biomaterialsmentioning

confidence: 99%

The Edifice of Vasculature-On-Chips: A Focused Review on the Key Elements and Assembly of Angiogenesis Models

Lim,

Fang,

Bupphathong

et al. 2024

ACS Biomater. Sci. Eng.

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The conception of vascularized organ-on-a-chip models provides researchers with the ability to supply controlled biological and physical cues that simulate the in vivo dynamic microphysiological environment of native blood vessels. The intention of this niche research area is to improve our understanding of the role of the vasculature in health or disease progression in vitro by allowing researchers to monitor angiogenic responses and cell−cell or cell− matrix interactions in real time. This review offers a comprehensive overview of the essential elements, including cells, biomaterials, microenvironmental factors, microfluidic chip design, and standard validation procedures that currently govern angiogenesis-on-a-chip assemblies. In addition, we emphasize the importance of incorporating a microvasculature component into organ-on-chip devices in critical biomedical research areas, such as tissue engineering, drug discovery, and disease modeling. Ultimately, advances in this area of research could provide innovative solutions and a personalized approach to ongoing medical challenges.

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“…The measurement sensitivity was better than that of a traditional trans-well assay. Lei et al developed a perfusion three-dimensional (3D) cell culture microfluidic chip combined with real-time and non-invasive impedance monitoring [73]. This device can simulate complex 3D biological microenvironments to culture cells and monitor the impedance changes under different concentrations of drug stimulation through impedance measurements.…”

Section: Static Cell Analyzed By Electrical Impedance Spectroscopymentioning

confidence: 99%

A Review on Microfluidics-Based Impedance Biosensors

et al. 2023

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Electrical impedance biosensors are powerful and continuously being developed for various biological sensing applications. In this line, the sensitivity of impedance biosensors embedded with microfluidic technologies, such as sheath flow focusing, dielectrophoretic focusing, and interdigitated electrode arrays, can still be greatly improved. In particular, reagent consumption reduction and analysis time-shortening features can highly increase the analytical capabilities of such biosensors. Moreover, the reliability and efficiency of analyses are benefited by microfluidics-enabled automation. Through the use of mature microfluidic technology, complicated biological processes can be shrunk and integrated into a single microfluidic system (e.g., lab-on-a-chip or micro-total analysis systems). By incorporating electrical impedance biosensors, hand-held and bench-top microfluidic systems can be easily developed and operated by personnel without professional training. Furthermore, the impedance spectrum provides broad information regarding cell size, membrane capacitance, cytoplasmic conductivity, and cytoplasmic permittivity without the need for fluorescent labeling, magnetic modifications, or other cellular treatments. In this review article, a comprehensive summary of microfluidics-based impedance biosensors is presented. The structure of this article is based on the different substrate material categorizations. Moreover, the development trend of microfluidics-based impedance biosensors is discussed, along with difficulties and challenges that may be encountered in the future.

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Quantitative study of tumor angiogenesis in three-dimensional matrigel barrier using electric impedance measurement technique

Cited by 7 publications

References 40 publications

Exploring Endothelial Expansion on a Chip

Exploring Endothelial Expansion on a Chip

The Edifice of Vasculature-On-Chips: A Focused Review on the Key Elements and Assembly of Angiogenesis Models

A Review on Microfluidics-Based Impedance Biosensors

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