Quantitative study of the proportion of the pore volume of human fluorotic enamel filled by resin infiltrant

Sousa, Frederico Barbosa de; Lelis, Isabel Maria Porto; Figueiredo, Regina Célia Bressan Queiroz de; Pires, Andressa Cavalcanti; Gerlach, Raquel Fernanda

doi:10.1016/j.archoralbio.2017.06.017

Cited by 9 publications

(15 citation statements)

References 29 publications

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“…At the same histological points selected before, birefringence in water (24 h of water immersion) was measured using the single polscope [Meira et al, 2015;De Sousa et al, 2017]. Using mineral volumes measured previously, organic and water volumes were obtained by the interpretation of enamel birefringence with the equations that follow below.…”

Section: Organic and Water Volume Analysis (Total And Loosely Bound Wmentioning

confidence: 99%

“…After drying (48 h at 25 ° C and 50% of relative humidity), all lesions became opaque so that a mathematical approximation [Macena et al, 2014] was used to quantify loosely bound volume as described recently [Meira et al, 2015;De Sousa et al, 2017]:…”

Section: Organic and Water Volume Analysis (Total And Loosely Bound Wmentioning

confidence: 99%

“…After lesion formation, no acid-etching pretreatment was applied on the enamel lesions. Resin infiltration was monitored in real time using the single polscope with birefringence measurements performed at 1-min intervals for 10 min, after which resin was light-cured for 1 min [De Sousa et al, 2017].…”

Section: Organic and Water Volume Analysis (Total And Loosely Bound Wmentioning

confidence: 99%

“…Resin infiltration into subsurface lesions was analyzed performing Z scans under a confocal laser scanning microscope (model DMIRE2, Leica, Germany; excitation of 488 nm and emission peak at 510 nm) [De Sousa et al, 2017] exploring the autofluorescence of the resin infiltrant [Gan et al, 2015].…”

Section: Confocal Laser Scanning Microscopymentioning

confidence: 99%

“…The enamel birefringence value measured at the end of the resin infiltration period (after 10 min) was used to quantify the pore volume fraction occluded by the resin infiltrant (refractive index of 1.46) using the modified Sousa equation [De Sousa et al, 2017]:…”

Section: Analysis Of the Volume Occluded By Resin Infiltrantmentioning

confidence: 99%

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High Amount of Organic Matter during Caries Formation Reduces Remineralization and Resin Infiltration of Enamel Caries

et al. 2018

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The amount of organic material in the cariogenic environment correlates with the amount of organic material incorporated in carious enamel. The incorporated organic material may be expected to reduce the pore volumes available for remineralization and resin infiltration, but these expected outcomes have not yet been quantified. We tested the effect of the amount of organic content in the cariogenic agent on remineralization and the resin-occluded pore volume in artificial subsurface enamel caries. An acid gel (organic-rich; G1) and an aqueous solution (organic-poor; G2) were used to induce subsurface lesions in human enamel. Undemineralized histological sections were prepared, microradiographed, and then submitted to resin infiltration in vitro. The enamel component volumes (mineral, organic, remineralizable [total water volume], loosely and firmly bound water volumes, and resin-occluded volume) were measured (by microradiography and polarizing microscopy) at histological sites (n = 38, G1; n = 34, G2). The main outcomes were the differences between the experimental and the predicted volumes (Δremineralizable and Δresin-occluded volumes). Resin infiltration was confirmed by confocal scanning laser microscopy. Compared to G2, G1 presented more incorporated organic volume and lower Δremineralizable volume (p = 0.003; Hedges g = 0.66; power = 0.87), a lower increase in loosely bound water volume (p = 0.0013; Hedges g = 0.74; power = 0.93), a lower remineralization volume in the surface layer (p = 0.017; Hedges g = 0.68; power = 0.8), and a lower Δresin-occluded volume (p = 0.0015; Hedges g = 0.73; power = 0.92). In conclusion, the higher amount of organic matter in the cariogenic gel negatively affected remineralization and the resin-occluded volume in subsurface lesions.

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Section: Organic and Water Volume Analysis (Total And Loosely Bound Wmentioning

confidence: 99%

Section: Organic and Water Volume Analysis (Total And Loosely Bound Wmentioning

confidence: 99%

Section: Organic and Water Volume Analysis (Total And Loosely Bound Wmentioning

confidence: 99%

Section: Confocal Laser Scanning Microscopymentioning

confidence: 99%

Section: Analysis Of the Volume Occluded By Resin Infiltrantmentioning

confidence: 99%

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High Amount of Organic Matter during Caries Formation Reduces Remineralization and Resin Infiltration of Enamel Caries

et al. 2018

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Natural enamel caries in quinoline: Volumetric data and the pattern of infiltration

Brito

Meira

Sousa

2017

Microscopy Res & Technique

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This study aimed to test the hypotheses that (i) a parameter related to permeability, α (ratio of squared water volume by the nonmineral volume) is, among all major component volumes (mineral, water, and organic volumes) the best predictor of quinoline infiltration in natural enamel caries (NEC), and (ii) the pore volume fraction infiltrated by quinoline (V ) in NEC is much lower than previous estimates that neglected water and organic enamel volumes. Mineral and nonmineral volumes and α were measured at 341 histological points (from 20 approximal NEC lesions), and transport of quinoline was tracked by orientation-independent polarizing microscopy. R values of V were 0.596 (α ), 0.033 (mineral volume), 0.474 (water volume), and 0.011 (organic volume). V values were 23% (body of the lesion), 7% (dark zone), and 9% (translucent zone), lower than previous estimates (with high effect size). Transport of quinoline occurred both parallelly and perpendicularly to prism paths, and dark zones were seen where only transport parallel to prisms occurred. In conclusion, α was the main predictor of quinoline infiltration, but it differed from the water volume with a small effect size, and the pore volume fraction with quinoline was much lower than previous estimates.

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In vitro effect of a resin infiltrant on different artificial caries-like enamel lesions

Freitas

Nunes

Comar

et al. 2018

Archives of Oral Biology

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Quantitative study of the proportion of the pore volume of human fluorotic enamel filled by resin infiltrant

Abstract: [Formula: see text] and V correlated well, but lacked good agreement. Organic matter, firmly bound water and air remained in enamel pores after resin infiltration.

Cited by 9 publications

References 29 publications

High Amount of Organic Matter during Caries Formation Reduces Remineralization and Resin Infiltration of Enamel Caries

High Amount of Organic Matter during Caries Formation Reduces Remineralization and Resin Infiltration of Enamel Caries

Natural enamel caries in quinoline: Volumetric data and the pattern of infiltration

In vitro effect of a resin infiltrant on different artificial caries-like enamel lesions

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