Quantitative Screening of Cell‐Surface Gangliosides by Nondestructive Extraction and Hydrophobic Collection

Abstract

A screening strategy involving designed extractors and collectors was used for the nondestructive quantitation of gangliosides on cell surfaces. The extractors were constructed by functionalizing maleimide silica bubbles with a DNA probe, which contains an endonuclease cleavage site and a boronic acid end to extract cell-surface sialic acid-containing compounds through simple centrifugation. After the extractors containing the extracted compounds were incubated with endonuclease, the released oligonucleotide-gangliosides were selectively collected by silanized collector bubbles through hydrophobic interactions. The in vitro fluorescent signals from the collectors were used for the quantitation of cell-surface gangliosides. By combining with sialidase cleavage, a protocol for the identification of ganglioside subtypes was developed. The successful monitoring of the regeneration of cell-surface gangliosides demonstrates the potential of this strategy in probing related biological processes.

DNA nanotweezers for stabilizing and dynamically lighting up a lipid raft on living cell membranes and the activation of T cells

“…2 Recently, DNA nanostructures with lipidic anchors were demonstrated to be promising membrane protein mimics that enable one to monitor molecular encounter events on living cell membranes. [33][34][35][36][37][38][39][40][41] In the present study, we demonstrate that DNA nanotweezers could stabilize and dynamically light up lipid ras on living cell membranes. The proposed DNA nanotweezers create an exogenous cholesterol-rich region on living cell membranes, which results in the recruitment of raassociated lipids and proteins, and possibly also endogenous cholesterol.…”

In Situ Cellular Glycan Analysis

“…By modifying PBA onto nanostructures such as gold nanoflowers or nanoclusters, cell surface Sia can be sensitively detected by SERS or fluorescence. In addition, Sia-contained glycolipids, i.e., gangliosides, can also be quantitated with a PBA functionalized silica bubble.…”

Bioorthogonal Microbubbles with Antifouling Nanofilm for Instant and Suspended Enrichment of Circulating Tumor Cells

“…Recently, low-cost hollow glass microbubbles (GBs) emerge as another platform to enable cell isolation, featuring low cost, biocompatibility, easy modification, and self-floating. ,− Notably, the microscale GBs can avoid the unordered stacking of nanosized magnetic beads and potential endocytosis in cell enrichment, thereby are well suited for CTC capture. Nevertheless, their harsh surface treatment (with concentrated strong acids), strong nonspecific adsorption, and low surface-to-volume ratio result in a limited interface–cell contact footprint and largely degrade their advantages in high-efficiency isolation of target cells and the downstream single-cell 3D culture.…”